Q9I8N6 · CSF1R_DANRE
- ProteinMacrophage colony-stimulating factor 1 receptor
- Genecsf1r
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids977 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Activates several signaling pathways in response to ligand binding (By similarity).
Catalytic activity
- L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H+
Activity regulation
Present in an inactive conformation in the absence of bound ligand. CSF1 binding leads to dimerization and activation by autophosphorylation on tyrosine residues (By similarity).
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 590-598 | ATP (UniProtKB | ChEBI) | |||
Binding site | 618 | ATP (UniProtKB | ChEBI) | |||
Active site | 781 | Proton acceptor | |||
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMacrophage colony-stimulating factor 1 receptor
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionQ9I8N6
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Single-pass type I membrane protein
Note: The autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 19-519 | Extracellular | |||
Transmembrane | 520-540 | Helical | |||
Topological domain | 541-977 | Cytoplasmic | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain, disulfide bond, glycosylation, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-18 | ||||
Chain | PRO_0000249006 | 19-977 | Macrophage colony-stimulating factor 1 receptor | ||
Disulfide bond | 48↔92 | ||||
Glycosylation | 98 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 101 | N-linked (GlcNAc...) asparagine | |||
Disulfide bond | 138↔187 | ||||
Glycosylation | 154 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 163 | N-linked (GlcNAc...) asparagine | |||
Disulfide bond | 234↔289 | ||||
Glycosylation | 244 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 286 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 298 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 361 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 424 | N-linked (GlcNAc...) asparagine | |||
Disulfide bond | 430↔495 | ||||
Glycosylation | 455 | N-linked (GlcNAc...) asparagine | |||
Modified residue | 563 | Phosphotyrosine; by autocatalysis | |||
Modified residue | 701 | Phosphotyrosine; by autocatalysis | |||
Modified residue | 725 | Phosphotyrosine; by autocatalysis | |||
Modified residue | 812 | Phosphotyrosine; by autocatalysis | |||
Modified residue | 929 | Phosphotyrosine; by autocatalysis | |||
Modified residue | 974 | Phosphotyrosine; by autocatalysis | |||
Post-translational modification
Autophosphorylated in response to CSF1 binding. autophosphorylation, leading to its degradation.
Ubiquitinated. Becomes rapidly polyubiquitinated after autophosphorylation, leading to its degradation (By similarity).
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Monomer. Homodimer. Interacts with CSF1 (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 22-109 | Ig-like C2-type 1 | |||
Domain | 120-198 | Ig-like C2-type 2 | |||
Domain | 213-305 | Ig-like C2-type 3 | |||
Domain | 316-407 | Ig-like C2-type 4 | |||
Domain | 408-513 | Ig-like C2-type 5 | |||
Region | 544-576 | Regulatory juxtamembrane domain | |||
Domain | 584-917 | Protein kinase | |||
Region | 799-821 | Activation loop | |||
Compositional bias | 919-938 | Polar residues | |||
Region | 919-977 | Disordered | |||
Compositional bias | 942-956 | Basic and acidic residues | |||
Domain
The juxtamembrane domain functions as autoinhibitory region. Phosphorylation of tyrosine residues in this region leads to a conformation change and activation of the kinase (By similarity).
The activation loop plays an important role in the regulation of kinase activity. Phosphorylation of tyrosine residues in this region leads to a conformation change and activation of the kinase (By similarity).
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length977
- Mass (Da)110,188
- Last updated2000-10-01 v1
- MD5 ChecksumE6295AFB0C04E7178B9DA52E2D5894BC
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8M9QKN6 | A0A8M9QKN6_DANRE | csf1ra | 977 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 919-938 | Polar residues | |||
Compositional bias | 942-956 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF240639 EMBL· GenBank· DDBJ | AAF76872.1 EMBL· GenBank· DDBJ | mRNA |