Q9I767 · 5NTD_PSEAE
- Protein5'-nucleotidase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids221 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Specifically dephosphorylates nucleoside 5'-monophosphates to nucleosides and inorganic phosphate. Displays high activity toward 5'-UMP and 5'-IMP, significant activity against 5'-XMP and 5'-TMP, and low activity against 5'-CMP.
Catalytic activity
- a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate
Cofactor
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )
Note: Divalent metal cation. Prefers Mn2+ over Mg2+.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.39 mM | 5'-UMP |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
3.14 μmol/min/mg | with 5'-UMP as substrate |
pH Dependence
Optimum pH is 7.4.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 14 | Nucleophile | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Molecular Function | protein tyrosine kinase activity | |
Molecular Function | XMP 5'-nucleosidase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5'-nucleotidase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionQ9I767
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000064390 | 1-221 | 5'-nucleotidase | |||
Sequence: MRDAALRYPNILFDLDGTLTDPREGITRSVQFALARLGIDEPDLARLEHFIGPPLLQCFMQTYGFDEARAWEAVNHYRERFRVTGLYENRVFDGIPELLEALVGRGHTLYVATSKPGVFAREIARHFAFDRHFKAIYGSELDGTRTHKEELIRHLLDSEGLAAEHCLMIGDRMHDLLGASRNGVACIGVGYGFGSEDELRAHQPTHYCADLAALRQVLESH |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length221
- Mass (Da)24,951
- Last updated2001-03-01 v1
- Checksum8885AF689CC828B1
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE004091 EMBL· GenBank· DDBJ | AAG03455.1 EMBL· GenBank· DDBJ | Genomic DNA |