Q9I4F5 · DCTA2_PSEAE
- ProteinC4-dicarboxylate transport protein 2
- GenedctA2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids436 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the inner membrane.
Miscellaneous
The DctPQM carrier is more efficient than the DctA carrier for the utilization of succinate at micromolar concentrations, whereas DctA is the major transporter at millimolar concentrations.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | fumarate transmembrane transporter activity | |
Molecular Function | malate:proton symporter activity | |
Molecular Function | succinate transmembrane transporter activity | |
Biological Process | C4-dicarboxylate transport | |
Biological Process | dicarboxylic acid transport | |
Biological Process | L-aspartate transmembrane transport |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameC4-dicarboxylate transport protein 2
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionQ9I4F5
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 14-34 | Helical | ||||
Sequence: VLVAIAIGIALGHWYPETAVA | ||||||
Transmembrane | 45-65 | Helical | ||||
Sequence: LIKMAIAPIIFCTVVTGIAGM | ||||||
Transmembrane | 77-97 | Helical | ||||
Sequence: MALLYFEVVSTVALIIGLVVV | ||||||
Transmembrane | 142-162 | Helical | ||||
Sequence: VVGAFANGDILQVLFFSVLFG | ||||||
Transmembrane | 198-218 | Helical | ||||
Sequence: PIGAFGAMAFTIGAYGVGSLV | ||||||
Transmembrane | 223-243 | Helical | ||||
Sequence: LMLCFYITCILFVLIVLGGIA | ||||||
Transmembrane | 290-310 | Helical | ||||
Sequence: VVGLVIPTGYSFNLDGTSIYL | ||||||
Transmembrane | 331-351 | Helical | ||||
Sequence: ITLLLVLLIASKGAAGVTGSG | ||||||
Transmembrane | 353-373 | Helical | ||||
Sequence: IVLAATLSAVGHLPVAGLALI |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Inactivation of the gene causes a growth defect in minimal media supplemented with succinate, fumarate or malate. The dctA-dctPQM double mutant shows no growth on malate and fumarate and residual growth on succinate.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000202098 | 1-436 | C4-dicarboxylate transport protein 2 | |||
Sequence: MTKQPFYKSLYVQVLVAIAIGIALGHWYPETAVAMKPFGDGFVKLIKMAIAPIIFCTVVTGIAGMQSMKSVGKTGGMALLYFEVVSTVALIIGLVVVNVVQPGAGMHVDPNTLDTSKIAAYAAAGEKQSTVDFLMNVIPGTVVGAFANGDILQVLFFSVLFGYALHRLGSYGKPVFEFIERVSHVMFNIINVIMKVAPIGAFGAMAFTIGAYGVGSLVQLGQLMLCFYITCILFVLIVLGGIARAHGFSILRFIRYIREELLIVLGTSSSESALPRMIDKMEKLGCNKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATDTPMDITHQITLLLVLLIASKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNGVATVVVSKWCKQLDEGTLQRELAGEGNASSPASDIPVGGREAV |
Proteomic databases
Expression
Induction
Expression is maximal in early exponential growth phase and declines with cell density to reach a plateau in the stationary growth phase. Induced by the C4-dicarboxylates succinate, fumarate and malate. Positively regulated by RpoN and the DctB/DctD two-component system. Negatively autoregulated.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 414-436 | Disordered | ||||
Sequence: ELAGEGNASSPASDIPVGGREAV |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length436
- Mass (Da)45,994
- Last updated2001-03-01 v1
- ChecksumDCA564DB31A5AC07
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE004091 EMBL· GenBank· DDBJ | AAG04572.1 EMBL· GenBank· DDBJ | Genomic DNA |