Q9I3F5 · ACNA_PSEAE

Function

function

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The apo form of AcnA functions as a RNA-binding regulatory protein.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Organic acid metabolism; propanoate degradation.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site454[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site520[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site523[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Molecular Function2-methylisocitrate dehydratase activity
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionaconitate hydratase activity
Molecular Functioniron ion binding
Molecular Functioniron-responsive element binding
Molecular FunctionmRNA 3'-UTR binding
Molecular FunctionmRNA binding
Biological Processanaerobic respiration
Biological Processcitrate metabolic process
Biological Processpropionate metabolic process, methylcitrate cycle
Biological Processresponse to oxidative stress
Biological Processtricarboxylic acid cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Aconitate hydratase A
  • EC number
  • Short names
    ACN
    ; Aconitase
  • Alternative names
    • (2R,3S)-2-methylisocitrate dehydratase
    • (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase
    • Iron-responsive protein-like
      (IRP-like
      )
    • Probable 2-methyl-cis-aconitate hydratase
      (EC:4.2.1.99
      ) . EC:4.2.1.99 (UniProtKB | ENZYME | Rhea)
    • RNA-binding protein

Gene names

    • Name
      acnA
    • Ordered locus names
      PA1562

Organism names

Accessions

  • Primary accession
    Q9I3F5

Proteomes

Organism-specific databases

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002877391-910Aconitate hydratase A

Proteomic databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the aconitase/IPM isomerase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    910
  • Mass (Da)
    99,148
  • Last updated
    2001-03-01 v1
  • Checksum
    C65F23CDB6FA0E1C
MPALDSLKTLRSLAVDGKTYHYYSLPEAARTLGDLGKLPMSLKVLLENLLRWEDGSTVTGDDLKALAGWLRERRSDREIQYRPARVLMQDFTGVPAVVDLAAMRAAMAKAGGDPQKINPLSPVDLVIDHSVMVDKFASESAFEQNVEIEMQRNGERYAFLRWGQNAFDNFSVVPPGTGICHQVNLEYLGRTVWTKDEDGRTYAFPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLTGKLREGITATDLVLTVTQMLRKKGVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGFFPVDEITLGYLRLSGRPESTVKLVEAYSKEQGLWREKGHEPVFTDTLHLDMGEVEASLAGPKRPQDRVALQNVASAFNEFLGLQLHPSSTEEGRLLSEGGGGTAVGANAAFGEIDYQHDGQTHRLKNGAVVIAAITSCTNTSNPSVMMAAGLLAKKAVEKGLQRKPWVKSSLAPGSKVVTDYFKAAGLTRYLDELGFDLVGYGCTTCIGNSGPLLEPIEKAIQQADLTVASVLSGNRNFEGRVHPLVKTNWLASPPLVVAYALAGSVRINLSEEPLGTGKDGQPVYLKDIWPSQKEIAEAIQKVDTEMFHKEYAEVFAGDEKWQAIQVPQSDTYEWQADSTYIQHPPFFEHIAEAPPAIADVEQARVLAVLGDSVTTDHISPAGNIKADSPAGRYLREHGVEPKDFNSYGSRRGNHEVMMRGTFANIRIKNEMLGGEEGGNTLYVPSGEKLAIYDAAMRYQEDGTPLVIVAGKEYGTGSSRDWAAKGTNLLGVKAVIAESFERIHRSNLVGMGVLPLQFENGQDRKSLKLTGKEVLNIRGLGGELKPHMPLSVEVTREDGSQDSFKVLCRIDTLNEVEYFKAGGILHYVLRSML

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE004091
EMBL· GenBank· DDBJ
AAG04951.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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