Q9HZX3 · TGPA_PSEAE
- ProteinProtein-glutamine gamma-glutamyltransferase
- GenetgpA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids668 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Displays transglutaminase activity (TGase) in vitro. Plays a critical role in the viability of P.aeruginosa. Might contribute to an essential function linked to the cell wall.
Miscellaneous
Unlike Guinea pig TGase, TGase activity is not affected by EDTA addition, suggesting that TGase activity is Ca2+-independent, similar to members of the bacterial transglutaminase family.
Catalytic activity
- L-glutaminyl-[protein] + L-lysyl-[protein] = [protein]-L-lysyl-N6-5-L-glutamyl-[protein] + NH4+
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 404 | Nucleophile | ||||
Sequence: C | ||||||
Active site | 448 | |||||
Sequence: H | ||||||
Active site | 464 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | protein-glutamine gamma-glutamyltransferase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein-glutamine gamma-glutamyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionQ9HZX3
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-6 | Cytoplasmic | ||||
Sequence: MNAIPR | ||||||
Transmembrane | 7-27 | Helical | ||||
Sequence: VALVWLLVAQVLVILPHLAYM | ||||||
Topological domain | 28-50 | Periplasmic | ||||
Sequence: PLWIAAMWLGCAAWRVQVFRMRA | ||||||
Transmembrane | 51-71 | Helical | ||||
Sequence: GYPRAWVKLALALLAGAGVWL | ||||||
Topological domain | 72-74 | Cytoplasmic | ||||
Sequence: SRG | ||||||
Transmembrane | 75-95 | Helical | ||||
Sequence: SLVGLDAGAVLLIAAFILKLV | ||||||
Topological domain | 96-103 | Periplasmic | ||||
Sequence: EMKTRRDA | ||||||
Transmembrane | 104-124 | Helical | ||||
Sequence: LVLVFLGFFAVVVGYLFDDGF | ||||||
Transmembrane | 125-145 | Helical | ||||
Sequence: LAALYSLLPVTALLAALIGLQ | ||||||
Topological domain | 146-158 | Cytoplasmic | ||||
Sequence: QSAFASRPWPTLR | ||||||
Transmembrane | 159-179 | Helical | ||||
Sequence: LAGGLLLQALPLMLLLFLFFP | ||||||
Topological domain | 180-548 | Periplasmic | ||||
Sequence: RLGPLWSLPMPGNKGVTGLSESMAPGDIAELGRSAELAFRVRFEGALPPREQLYWRALTMERFDGRRWAQAPQWSGEDALHWQKRGPELRYDVIMQPSSQPWLFALDVAQTDQTDTRLMSDFHLQRRQPVEQRLFYRVSSWPQALRESSIDPRTRWRNLQLPMHGNPRARALADELRQAHAQPQALVAALLQRFNHEPFAYTLKPPATGADGVDDFLFDTRSGFCAHYAGAMAFVLRAAGIPARVVAGYQGGELNPAGNYLLVHQFDAHAWVEYWQPEQGWLSVDPTYQVAPERIEQGLEQALAGDSEYLADAPLSPLRYRGLPWLNDMRLAWDSLNYGWQRWVLAYQGEQQGAFLQRWFGGLDPTRLG | ||||||
Transmembrane | 549-569 | Helical | ||||
Sequence: LLLGAAAILSVGLLALFLLKP | ||||||
Topological domain | 570-668 | Cytoplasmic | ||||
Sequence: WQGRGDLRSRQLRRFERLLEMHGLRRSPGEGLRSYGERAARVLPAQAPAIAAFVGAFEAQRYGHGGADDPGLRLRALRRALPWRLVRTPTRDGRGEEQA |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Disruption experiments show that tgpA is essential.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000422419 | 1-668 | Protein-glutamine gamma-glutamyltransferase | |||
Sequence: MNAIPRVALVWLLVAQVLVILPHLAYMPLWIAAMWLGCAAWRVQVFRMRAGYPRAWVKLALALLAGAGVWLSRGSLVGLDAGAVLLIAAFILKLVEMKTRRDALVLVFLGFFAVVVGYLFDDGFLAALYSLLPVTALLAALIGLQQSAFASRPWPTLRLAGGLLLQALPLMLLLFLFFPRLGPLWSLPMPGNKGVTGLSESMAPGDIAELGRSAELAFRVRFEGALPPREQLYWRALTMERFDGRRWAQAPQWSGEDALHWQKRGPELRYDVIMQPSSQPWLFALDVAQTDQTDTRLMSDFHLQRRQPVEQRLFYRVSSWPQALRESSIDPRTRWRNLQLPMHGNPRARALADELRQAHAQPQALVAALLQRFNHEPFAYTLKPPATGADGVDDFLFDTRSGFCAHYAGAMAFVLRAAGIPARVVAGYQGGELNPAGNYLLVHQFDAHAWVEYWQPEQGWLSVDPTYQVAPERIEQGLEQALAGDSEYLADAPLSPLRYRGLPWLNDMRLAWDSLNYGWQRWVLAYQGEQQGAFLQRWFGGLDPTRLGLLLGAAAILSVGLLALFLLKPWQGRGDLRSRQLRRFERLLEMHGLRRSPGEGLRSYGERAARVLPAQAPAIAAFVGAFEAQRYGHGGADDPGLRLRALRRALPWRLVRTPTRDGRGEEQA |
Proteomic databases
Expression
Induction
Is expressed in both exponential and stationary phases of growth. Appears to be part of an operon together with PA2874 and PA2875.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length668
- Mass (Da)74,807
- Last updated2001-03-01 v1
- Checksum646CD6D34F6E96D4
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE004091 EMBL· GenBank· DDBJ | AAG06261.1 EMBL· GenBank· DDBJ | Genomic DNA |