Q9HZJ2 · FADB_PSEAE
- ProteinFatty acid oxidation complex subunit alpha
- GenefadB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids715 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.
Catalytic activity
- a (3S)-3-hydroxyacyl-CoA + NAD+ = a 3-oxoacyl-CoA + H+ + NADH
Pathway
Lipid metabolism; fatty acid beta-oxidation.
Features
Showing features for site, binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 120 | Important for catalytic activity | ||||
Sequence: E | ||||||
Site | 140 | Important for catalytic activity | ||||
Sequence: E | ||||||
Binding site | 297 | substrate | ||||
Sequence: D | ||||||
Binding site | 325 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Binding site | 344 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 401-403 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: VVE | ||||||
Binding site | 408 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 430 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 451 | For 3-hydroxyacyl-CoA dehydrogenase activity | ||||
Sequence: H | ||||||
Binding site | 454 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 501 | substrate | ||||
Sequence: N | ||||||
Binding site | 660 | substrate | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | fatty acid beta-oxidation multienzyme complex | |
Molecular Function | 3-hydroxybutyryl-CoA epimerase activity | |
Molecular Function | delta(3)-delta(2)-enoyl-CoA isomerase activity | |
Molecular Function | enoyl-CoA hydratase activity | |
Molecular Function | long-chain-3-hydroxyacyl-CoA dehydrogenase activity | |
Molecular Function | NAD+ binding | |
Biological Process | fatty acid beta-oxidation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFatty acid oxidation complex subunit alpha
Including 2 domains:
- Recommended nameEnoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase
- EC number
- Recommended name3-hydroxyacyl-CoA dehydrogenase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionQ9HZJ2
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000109274 | 1-715 | Fatty acid oxidation complex subunit alpha | |||
Sequence: MIYQGKAITVKPLEGGIVELNFDLKGESVNKFNRLTLSELRAAVDAIKADASVKGVIVTSGKDVFIVGADITEFVDNFQLPDEELMAGNLEANKIFSDFEDLDVPTVAAINGIALGGGLEMCLAADFRVMSATAKVGLPEVKLGIYPGFGGTVRLPRLIGCDNAVEWIASGKENKAEDALKVGAVDAVVAPEQLQAAALDLAKRAVAGELDHKARRQPKLEKLKLNAIEQMMAFETAKGFVAGQAGPNYPAPVEAIKSIQKAANFGRDKALEVEAAGFVKLAKTSVAQSLIGLFLNDQELKKKAKKYDEVAKDVKLAAVLGAGIMGGGIAYQSALKGTPILMKDIREEGIQMGLNEAAKLLGKRVEKGRLTPAKMAEALNGIRPTMSYGDFGNVDIVVEAVVENPKVKQAVLAEVEGAVKEDAIIASNTSTISISLLAQALKRPENFCGMHFFNPVHMMPLVEVIRGEKTGETAIATTVAYAKKMGKSPIVVNDCPGFLVNRVLFPYFGGFAKLLSFGVDFVRIDKVMEKFGWPMGPAYLSDVVGIDTGHHGRDVMAEGFPDRMAVEGKTAVDVMYEANRLGQKNGKGFYAYETDKRGKPKKVTDPQAYEVLKPIVVEQREVTDEDIVNFMMIPLCLETVRCLEDGIVETAAEADMGLIYGIGFPPFRGGALRYIDSIGVAEFVALADKYAELGALYHPTAKLREMAKNGQKFFG |
Proteomic databases
Interaction
Subunit
Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-190 | Enoyl-CoA hydratase/isomerase | ||||
Sequence: MIYQGKAITVKPLEGGIVELNFDLKGESVNKFNRLTLSELRAAVDAIKADASVKGVIVTSGKDVFIVGADITEFVDNFQLPDEELMAGNLEANKIFSDFEDLDVPTVAAINGIALGGGLEMCLAADFRVMSATAKVGLPEVKLGIYPGFGGTVRLPRLIGCDNAVEWIASGKENKAEDALKVGAVDAVVA | ||||||
Region | 312-715 | 3-hydroxyacyl-CoA dehydrogenase | ||||
Sequence: KDVKLAAVLGAGIMGGGIAYQSALKGTPILMKDIREEGIQMGLNEAAKLLGKRVEKGRLTPAKMAEALNGIRPTMSYGDFGNVDIVVEAVVENPKVKQAVLAEVEGAVKEDAIIASNTSTISISLLAQALKRPENFCGMHFFNPVHMMPLVEVIRGEKTGETAIATTVAYAKKMGKSPIVVNDCPGFLVNRVLFPYFGGFAKLLSFGVDFVRIDKVMEKFGWPMGPAYLSDVVGIDTGHHGRDVMAEGFPDRMAVEGKTAVDVMYEANRLGQKNGKGFYAYETDKRGKPKKVTDPQAYEVLKPIVVEQREVTDEDIVNFMMIPLCLETVRCLEDGIVETAAEADMGLIYGIGFPPFRGGALRYIDSIGVAEFVALADKYAELGALYHPTAKLREMAKNGQKFFG |
Sequence similarities
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length715
- Mass (Da)76,954
- Last updated2001-03-01 v1
- Checksum7ED8573BE8F31101
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE004091 EMBL· GenBank· DDBJ | AAG06402.1 EMBL· GenBank· DDBJ | Genomic DNA |