Q9HZE0 · DHE2_PSEAE

Function

function

Involved in arginine catabolism by converting L-glutamate, into 2-oxoglutarate, which is then channeled into the tricarboxylic acid cycle. Can also utilize other amino acids of the glutamate family.

Catalytic activity

Activity regulation

Activity subject to allosteric control by arginine and citrate, which function as positive and negative effectors, respectively.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.13 μMNAD

pH Dependence

Optimum pH is 8 for amination, and 9 for deamination.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site851

GO annotations

AspectTerm
Molecular Functionglutamate dehydrogenase (NAD+) activity
Biological Processglutamate catabolic process to 2-oxoglutarate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NAD-specific glutamate dehydrogenase
  • EC number
  • Short names
    NAD-GDH
  • Alternative names
    • NAD(+)-dependent glutamate dehydrogenase

Gene names

    • Name
      gdhB
    • Ordered locus names
      PA3068

Organism names

Accessions

  • Primary accession
    Q9HZE0
  • Secondary accessions
    • Q7BHF2

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Reduced growth when arginine serve as the sole source of carbon and nitrogen.

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00003933692-1620NAD-specific glutamate dehydrogenase

Post-translational modification

Contains disulfide bonds (interchain).

Keywords

Proteomic databases

Expression

Induction

Expression induced by arginine in an ArgR-dependent manner (27-fold). Induced to a much lesser extent by ornithine, glutamate, and aspartate (1.7- to 5-fold).

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,620
  • Mass (Da)
    182,638
  • Last updated
    2001-03-01 v1
  • Checksum
    1AF2AE56BE3274C3
MAFFTAASKADFQHQLQTALAQHLGDKALPQVTLFAEQFFSLISLDELTQRRLSDLVGCTLSAWRLLERFDRDQPEVRVYNPDYEKHGWQSTHTAVEVLHPDLPFLVDSVRMELNRRGYSIHTLQTNVLSVRRSAKGELKEILPKGSQGKDVSQESLMYLEIDRCAHAGELRALEKAILEVLGEVRVTVADFEPMKAKARELLTWLGKAKLKVPAEELKEVRSYLEWLLDNHFTFLGYEEFSVADEADGGRMVYDEKSFLGLTRLLRAGLSKDDLHIEDYAVAYLREPVLLSFAKAAHPSRVHRPAYPDYVSIRELDGKGRVIRECRFMGLFTSSVYNESVNDIPFIRGKVAEVMRRSGFDTKAHLGKELAQVLEVLPRDDLFQTPVDELFSTALAIVRIQERNKIRVFLRKDPYGRFCYCLAYVPRDVYSTETRLKIQQVLMERLQASDCEFWTFFSESVLARVQFILRVDPKSRIDIDPARLEEEVIQACRSWQDDYSSLVVENLGEAKGTNVLADFPKGFPAGYRERFAPHFAVVDLQHLLSLSEQRPLVMSFYQPLAQGEQQLHCKLYHADTPLALSDVLPILENLGLRVLGEFPYRLRHQNGREYWIHDFAFTYAEGLDVDIQQLNEILQDAFVHIVSGDAENDAFNRLVLTANLPWRDVALLRAYARYLKQIRLGFDLGYIASALNAHTDIARELVRLFKTRFYLARKLTAEDLEDKQQKLEQAILGALDEVQVLNEDRILRRYLDLIKATLRTNFYQPDGNGQNKSYFSFKFNPKAIPELPRPVPKYEIFVYSPRVEGVHLRGGKVARGGLRWSDREEDFRTEVLGLVKAQQVKNAVIVPVGAKGGFVPRRLPLGGSRDEIQAEAIACYRIFISGLLDITDNLKEGEVVPPANVVRHDEDDPYLVVAADKGTATFSDIANGIAAEYGFWLGDAFASGGSAGYDHKGMGITAKGAWVSVQRHFRERGIDVQKDNISVIGIGDMAGDVFGNGLLMSDKLQLVAAFNHMHIFIDPNPDAASSFVERQRLFNLPRSSWADYDAKLISAGGGIFLRSAKSIAITPEMKARFDIQADRLAPTELIHALLKAPVDLLWNGGIGTYVKSSKETHADVGDKANDGLRVDGRELRAKVVGEGGNLGMTQLARVEFGLHGGANNTDFIDNAGGVDCSDHEVNIKILLNEVVQAGDMTEKQRNALLVKMTDAVGALVLGNNYKQTQALSLAQRRARERIAEYKRLMGDLEARGKLDRALEFLPSDEELAERISAGQGLTRAELSVLISYSKIDLKESLLKSLVPDDDYLTRDMETAFPALLAEKFGDAMRRHRLKREIVSTQIANDLVNHMGITFVQRLKESTGMSAANVAGAYVIVRDVFHLPHWFRQIENLDYQVPADIQLTLMDELMRLGRRATRWFLRSRRNELDAARDVAHFGPRIAALGLKLNELLEGPTRELWQARYQTYVDAGVPELLARMVAGTSHLYTLLPIIEASDVTGQDTAEVAKAYFAVGSALDLTWYLQQITNLPVENNWQALAREAFRDDLDWQQRAITVSVLQMQDGPKEVEARVGLWLEQHLPLVERWRAMLVELRAASGTDYAMYAVANRELMDLAQSSQHGVCIP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF315586
EMBL· GenBank· DDBJ
AAG53963.1
EMBL· GenBank· DDBJ
Genomic DNA
AE004091
EMBL· GenBank· DDBJ
AAG06456.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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