Q9HYL2 · NOSZ_PSEAE

Function

function

Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Note: Binds 2 calcium ions per subunit.
Cu cation (UniProtKB | Rhea| CHEBI:23378 )

Note: Binds 6 Cu cations per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction.

Pathway

Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 4/4.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site127Cu cation Z2 (UniProtKB | ChEBI)
Binding site128Cu cation Z3 (UniProtKB | ChEBI)
Binding site176Cu cation Z2 (UniProtKB | ChEBI)
Binding site254Ca2+ 2 (UniProtKB | ChEBI)
Binding site257Ca2+ 2 (UniProtKB | ChEBI)
Binding site265Ca2+ 2 (UniProtKB | ChEBI)
Binding site271Ca2+ 2 (UniProtKB | ChEBI)
Binding site322Ca2+ 2 (UniProtKB | ChEBI)
Binding site324Cu cation Z1 (UniProtKB | ChEBI)
Binding site380Cu cation Z1 (UniProtKB | ChEBI)
Binding site431Cu cation Z3 (UniProtKB | ChEBI)
Binding site452Ca2+ 1 (UniProtKB | ChEBI)
Binding site467Ca2+ 1 (UniProtKB | ChEBI)
Binding site492Cu cation Z4 (UniProtKB | ChEBI)
Binding site581Cu cation A1 (UniProtKB | ChEBI)
Binding site616Cu cation A1 (UniProtKB | ChEBI)
Binding site616Cu cation A2 (UniProtKB | ChEBI)
Binding site618Cu cation A2 (UniProtKB | ChEBI)
Binding site620Cu cation A1 (UniProtKB | ChEBI)
Binding site620Cu cation A2 (UniProtKB | ChEBI)
Binding site624Cu cation A2 (UniProtKB | ChEBI)
Binding site627Cu cation A1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmembrane
Cellular Componentperiplasmic space
Molecular Functioncalcium ion binding
Molecular Functioncopper ion binding
Molecular Functioncytochrome-c oxidase activity
Molecular Functionnitrous-oxide reductase activity
Biological Processdenitrification pathway

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nitrous-oxide reductase
  • EC number
  • Alternative names
    • N(2)OR
    • N2O reductase

Gene names

    • Name
      nosZ
    • Ordered locus names
      PA3392

Organism names

Accessions

  • Primary accession
    Q9HYL2

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-49Tat-type signal
ChainPRO_000001982850-636Nitrous-oxide reductase

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region302-322Disordered
Region540-636COX2-like

Sequence similarities

Belongs to the NosZ family.
In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    636
  • Mass (Da)
    70,660
  • Last updated
    2001-01-11 v1
  • Checksum
    F3D66D519CF220BF
MSDDTKSPHEETHGLNRRGFLGASALTGAAALVGASALGSAVVGREARAAGKGERSKAEVAPGELDEYYGFWSGGHSGEVRVLGVPSMRELMRIPVFNVDSATGWGLTNESKRVLGDSARFLNGDCHHPHISMTDGKYDGKYLFINDKANSRVARIRLDVMKCDRIVTIPNVQAIHGLRLQKVPHTRYVFCNAEFIIPHPNDGSTFDLSGDNAFTLYNAIDAETMEVAWQVIVDGNLDNTDMDYSGRFAASTCYNSEKAVDLGGMMRNERDWVVVFDIPRIEAEIKAKRFVTLGDSKVPVVDGRRKDGKDSPVTRYIPVPKNPHGLNTSPDGKYFIANGKLSPTCTMIAIERLGDLFAGKLADPRDVVVGEPELGLGPLHTTFDGRGNAYTTLFIDSQLVKWNLADAVRAYKGEKVDYIRQKLDVQYQPGHNHATLCETSEADGKWIVVLSKFSKDRFLPTGPLHPENDQLIDISGEEMKLVHDGPTFAEPHDCILARRDQIKTRKIWDRKDPFFAETVKRAEKDGIDLMKDNKVIREGNKVRVYMVSMAPSFGLTEFKVKQGDEVTVTITNLDEIEDVTHGFVMVNHGVCMEISPQQTSSITFVADKPGVHWYYCSWFCHALHMEMCGRMLVEKA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE004091
EMBL· GenBank· DDBJ
AAG06780.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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