Q9HWG4 · PCHF_PSEAE

Function

function

Involved in the biosynthesis of the siderophore pyochelin (PubMed:11208777, PubMed:9846750).
Adenylates L-cysteine and loads it onto its peptidyl carrier domain via a thioester linkage to the phosphopanthetheine moiety (By similarity).
Then forms a peptide bond between the salicyl-thiazolinyl intermediate bound to the second carrier domain of PchE and the cysteine bound to its own peptidyl carrier domain to form the salicyl-thiazolinyl-cysteinyl-S-PCP2 intermediate. It subsequently cyclizes the C-terminal cysteine to form the second thiazoline heterocycle in the salicyl-thiazolinyl-thiazolinyl-S-PCP2 intermediate (By similarity).
When this intermediate is released by the action of a thioesterase, it produces the tricyclic acid hydroxyphenyl-thiazolyl-thiazolinyl-carboxylic acid (HPTT-COOH), an advanced intermediate containing the aryl-4,2-bis-heterocyclic skeleton of the bithiazoline class of siderophores (By similarity).

Catalytic activity

Cofactor

pantetheine 4'-phosphate (UniProtKB | Rhea| CHEBI:47942 )

Pathway

Siderophore biosynthesis.

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionligase activity
Molecular Functionphosphopantetheine binding
Biological Processamino acid activation for nonribosomal peptide biosynthetic process
Biological Processsecondary metabolite biosynthetic process
Biological Processtoxin biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyochelin synthetase PchF
  • EC number
  • Alternative names
    • L-cysteine--[L-cysteinyl-carrier protein] ligase
    • Nonribosomal peptide synthetase PchF

Gene names

    • Name
      pchF
    • Ordered locus names
      PA4225

Organism names

Accessions

  • Primary accession
    Q9HWG4
  • Secondary accessions
    • O85740

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Mutant can still form salicylate and Dha, but is no longer able to synthesize pyochelin.

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004548281-1809Pyochelin synthetase PchF
Modified residue1442O-(pantetheine 4'-phosphoryl)serine

Keywords

Proteomic databases

Expression

Induction

Expression of the pchEF operon is strictly dependent on the PchR regulator and is induced by extracellular pyochelin, the end product of the pathway. Repressed by Fur and iron.

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region69-490Condensation/cyclization
Region520-915Adenylation
Domain1407-1488Carrier
Region1584-1797Thioesterase

Domain

Modular protein that contains a condensation/cyclization domain involved in the cyclization of the cysteine, an adenylation domain which activates the cysteine residue into an aminoacyl-AMP ester, a peptidyl carrier protein (PCP2) domain which bears a phosphopantetheinyl arm to attach the activated cysteine and a thioesterase domain (TE) that may release the newly synthesized peptide from the enzyme.

Sequence similarities

Belongs to the NRP synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,809
  • Mass (Da)
    197,084
  • Last updated
    2001-03-01 v1
  • Checksum
    39978C86A1BB5F0B
MSLGELLETCRSRRIELWSEAGRLRYRAPQGALDAGLAERLRAEREALLEHLEGGPGWRAEPDMAHQRFPLTPVQAAYVLGRQAAFDYGGNACQLYAEYDWPADTDPARLEAAWNAMVERHPMLRAVIEDNAWQRVLPEVPWQRLTVHACAGLDEAAFQAHLERVRERLDHACAALDQWPVLRPELSIGRDACVLHCSVDFTLVDYASLQLLLGEWRRRYLDPQWTAEPLEATFRDYVGVEQRRRQSPAWQRDRDWWLARLDALPGRPDLPLRVQPDTRSTRFRHFHARLDEAAWQALGARAGEHGLSAAGVALAAFAETIGRWSQAPAFCLNLTVLNRPPLHPQLAQVLGDFTALSLLAVDSRHGDSFVERARRIGEQMFDDLDHPTFSGVDLLRELARRRGRGADLMPVVFTSGIGSVQRLLGDGEAPRAPRYMISQTPQVWLDCQVTDQFGGLEIGWDVRLGLFPEGQAEAMFDDFVGLLRRLAQSPRAWTDGDATEPVEAPPQALPGSARSIAAGFAERALLTPDATAIHDAAGSYSYRQVAQHASALRRVLEAHGAGRGRRVAVMLPKSAAQLVAVIGILQAGAAYVPVDIRQPPLRRQAILASAEVVALVCLESDVPDVGCACVAIDRLAADSAWPPPPAAEVAADDLAYVIYTSGSTGTPKGVMLSHAAVSNTLLDINQRYGVDANDRVLGLAELSFDLSVYDFFGATAAGAQVVLPDPARGSDPSHWAELLERHAITLWNSVPAQGQMLIDYLESEPQRHLPGPRCVLWSGDWIPVSLPTRWWRRWPDSALFSLGGATEAAIWSIEQPIRPQHTELASIPYGRALRGQSVEVLDARGRRCPPGVRGEIHIGGVGLALGYAGDPQRTAERFVRHPDGRRLYRTGDLGRYLADGSIEFLGREDDQVKIRGHRIELAELDAALCAHPQVNLAATVVLGETHERSLASFVTLHAPVEAGEDPRTALDAVRQRAAQALRRDWGSEEGIAAAVAALDRACLASLAAWLAGSGLFASATPLDLATLCQRLGIAEARQRLLRHWLRQLEEGGYLRAEGEGWLGCAERPAQSPEDAWTAFAGCAPAALWPAELVAYLRDSAQSLGEQLAGRISPAALMFPQGSARIAEAMYSQGLHAQALHEAMAEAIAAIVERQPQRRWRLLELGAGTAAASRTVIARLAPLVQRGAEVDYLFTDVSSYFLAAARERFADQPWVRFGRFDMNGDLLDQGVAPHSVDILLSSGALNNALDTPALLAGLRELLSADAWLVIQELTREHNEISVSQSLMMENPRDLRDERRQLFVHTGQWLEWLAAQGGDLACGVVPPGSALDLLGYDVLLARCKTDRARLEPAELLAFVEARVPRYMLPAQLRVLERLPVTGNGKIDRKALTGFARQPQADLRHGVAQAPADELENALLALWREVLDNPSLGVEQDFFGAGGDSLLIAQLIARLRERLESARRHPFDRLLRWALSQPTPRGLAERLRSAPEEGRGPALAAARGVAPAPAGMSRAPLAEGAVALDPLVRLVPGEGVPRVLVHEGLGTLLPYRPLLRALGEGRPLLGLAVHDSDAYLAIPAEHLNACLGRRYAEALHRAGLREVDLLGYCSGGLVALETAKSLVQRGVRVRQLDIVSSYRIPYRVDDERLLLFSFAATLGLDTAALGFPAPERLGQAVQAALAQTPERLVAEALAGLPGLADLVALRGRVLQAASGSADAVSVERDTLYRLFCHSVRASQAEAPEPYVGALRLFVPDAGNPLVPRYAEALETQWRAAALGACGIHEVPGGHFDCLGEALAQSLSKPMPEEASR

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict799-805in Ref. 1; AAC83657

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF074705
EMBL· GenBank· DDBJ
AAC83657.1
EMBL· GenBank· DDBJ
Genomic DNA
AE004091
EMBL· GenBank· DDBJ
AAG07613.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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