Q9HR29 · Q9HR29_HALSA

Function

function

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site130O-(5'-phospho-DNA)-tyrosine intermediate

GO annotations

AspectTerm
Cellular Componentchromosome
Cellular Componentcytoplasm
Cellular ComponentDNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
Biological ProcessDNA topological change
Biological ProcessDNA-templated DNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA gyrase subunit A
  • EC number

Gene names

    • Name
      gyrA
    • Ordered locus names
      VNG_0889G

Organism names

Accessions

  • Primary accession
    Q9HR29

Proteomes

Subcellular Location

Keywords

PTM/Processing

Proteomic databases

Interaction

Subunit

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, coiled coil, motif.

TypeIDPosition(s)Description
Domain19-470DNA topoisomerase type IIA
Coiled coil442-490
Motif531-537GyrA-box

Sequence similarities

Belongs to the type II topoisomerase GyrA/ParC subunit family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    824
  • Mass (Da)
    90,223
  • Last updated
    2001-03-01 v1
  • Checksum
    CB55755219FE09F0
MSSESPDVPDDVADRVKNVRVDEEMEQSYIDYAMSVIAGRALPDVRDGLKPVHRRILYAMHESGVTSGSGHRKSSNIVGDTMGDYHPHGDSPIYDALVRMAQDFSMRYPLVDGQGNFGSVDGDPAAAMRYTEARMAPIAEELLTDIEKDTVAFQANYDDRLTEPAVLPSSFPNLLVNGSSGIAVGMSTNVPPHNLGEVIDATIHLIDTPDCTVLDLMEYVKGPDFPTAANIVGRADVKQAYQTGRGRVRMRAEYHVESNGRRDSIVITELPYQQNKSTLVERIADDVRDGKLEGITDLRDESDRNGVRIVIDLKQKANTDVVENRLLESHLEKTFSVINLALVDGEPKVLTLKELLAEYVSHRKEVVRRRSEHDLGEAEDRAHILDGRLTALENADEVVELIQDAEDRDAAKETLKSAFDFSQDQADHIVRMQLGSLTSMEAADIESEYEDVTARIERLEEILGSEQELLDVIKDELREIKTQYADERRTSFIEDTGTVADEDLIPEEDTVVVLSEGDYIKRVPAETFDAQHRGGKGIIGSDLKDGDRVSTVFTASTHDYLLCFTDQGQVYRLKVYQVPEMSRTARGTSAVNILDLDDGEEISAVVTADDLDFKADDEYLTMATRNGYVKRTSVGEFGNILSTGIIAIDLEDGDALADVEVTDGSHDVILGSEAGMAIRFDEGDVRAMGRNARGVRGMDLDAADRIAGVAAVESEDDRSLLTVTEFGYGKRTRVGEYRSQSRNGKGLVDIKTGDRNGDVVSVDAVGDDDSLVVMSADGQIIQMPVDEISTVGRNTKGVNIMAVGSGDEVAGVSVRAADQADGEE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE004437
EMBL· GenBank· DDBJ
AAG19329.1
EMBL· GenBank· DDBJ
Genomic DNA
BK010829
EMBL· GenBank· DDBJ
DAC78034.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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