Q9HR29 · Q9HR29_HALSA
- ProteinDNA gyrase subunit A
- GenegyrA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids824 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
Miscellaneous
Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.
Catalytic activity
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 130 | O-(5'-phospho-DNA)-tyrosine intermediate | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | cytoplasm | |
Cellular Component | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity | |
Biological Process | DNA topological change | |
Biological Process | DNA-templated DNA replication |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA gyrase subunit A
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageArchaea > Euryarchaeota > Stenosarchaea group > Halobacteria > Halobacteriales > Halobacteriaceae > Halobacterium > Halobacterium salinarum NRC-34001
Accessions
- Primary accessionQ9HR29
Proteomes
Subcellular Location
PTM/Processing
Proteomic databases
Interaction
Subunit
Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, coiled coil, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 19-470 | DNA topoisomerase type IIA | ||||
Sequence: VRVDEEMEQSYIDYAMSVIAGRALPDVRDGLKPVHRRILYAMHESGVTSGSGHRKSSNIVGDTMGDYHPHGDSPIYDALVRMAQDFSMRYPLVDGQGNFGSVDGDPAAAMRYTEARMAPIAEELLTDIEKDTVAFQANYDDRLTEPAVLPSSFPNLLVNGSSGIAVGMSTNVPPHNLGEVIDATIHLIDTPDCTVLDLMEYVKGPDFPTAANIVGRADVKQAYQTGRGRVRMRAEYHVESNGRRDSIVITELPYQQNKSTLVERIADDVRDGKLEGITDLRDESDRNGVRIVIDLKQKANTDVVENRLLESHLEKTFSVINLALVDGEPKVLTLKELLAEYVSHRKEVVRRRSEHDLGEAEDRAHILDGRLTALENADEVVELIQDAEDRDAAKETLKSAFDFSQDQADHIVRMQLGSLTSMEAADIESEYEDVTARIERLEEILGSEQELL | ||||||
Coiled coil | 442-490 | |||||
Sequence: AADIESEYEDVTARIERLEEILGSEQELLDVIKDELREIKTQYADERRT | ||||||
Motif | 531-537 | GyrA-box | ||||
Sequence: QHRGGKG |
Sequence similarities
Belongs to the type II topoisomerase GyrA/ParC subunit family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length824
- Mass (Da)90,223
- Last updated2001-03-01 v1
- ChecksumCB55755219FE09F0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE004437 EMBL· GenBank· DDBJ | AAG19329.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK010829 EMBL· GenBank· DDBJ | DAC78034.1 EMBL· GenBank· DDBJ | Genomic DNA |