Q9HL35 · PYRDB_THEAC
- ProteinDihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
- GenepyrD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids300 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the conversion of dihydroorotate to orotate with NAD+ as electron acceptor.
Catalytic activity
- (S)-dihydroorotate + NAD+ = orotate + NADH + H+
Cofactor
Note: Binds 1 FMN per subunit.
Pathway
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD+ route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 21 | FMN (UniProtKB | ChEBI) | |||
Binding site | 45 | substrate | |||
Binding site | 45-46 | FMN (UniProtKB | ChEBI) | |||
Binding site | 69-73 | substrate | |||
Binding site | 125 | FMN (UniProtKB | ChEBI) | |||
Binding site | 125 | substrate | |||
Active site | 128 | Nucleophile | |||
Binding site | 163 | FMN (UniProtKB | ChEBI) | |||
Binding site | 187 | FMN (UniProtKB | ChEBI) | |||
Binding site | 188-189 | substrate | |||
Binding site | 213 | FMN (UniProtKB | ChEBI) | |||
Binding site | 239-240 | FMN (UniProtKB | ChEBI) | |||
Binding site | 261-262 | FMN (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | dihydroorotate dehydrogenase (NAD+) activity | |
Molecular Function | NADP binding | |
Molecular Function | uracil binding | |
Biological Process | 'de novo' pyrimidine nucleobase biosynthetic process | |
Biological Process | 'de novo' UMP biosynthetic process | |
Biological Process | thymine catabolic process | |
Biological Process | uracil catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
- EC number
- Short namesDHOD B; DHODase B; DHOdehase B
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Candidatus Thermoplasmatota > Thermoplasmata > Thermoplasmatales > Thermoplasmataceae > Thermoplasma
Accessions
- Primary accessionQ9HL35
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000148418 | 1-300 | Dihydroorotate dehydrogenase B (NAD+), catalytic subunit | ||
Proteomic databases
Interaction
Subunit
Heterotetramer of 2 PyrK and 2 PyrD type B subunits.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length300
- Mass (Da)32,057
- Last updated2001-03-01 v1
- Checksum68150404D7163E8D
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL445064 EMBL· GenBank· DDBJ | CAC11547.1 EMBL· GenBank· DDBJ | Genomic DNA |