Q9HI21 · GVPF1_HALSA

Function

function

Might be involved in preventing aggregation of GvpA1 (Probable) (PubMed:33281806).
Proteins GvpF to GvpM might be involved in nucleating gas vesicle formation (Probable) (PubMed:15126480, PubMed:33281806).
A minor component of the gas vesicle, also found in soluble extracts (PubMed:15126480).
Gas vesicles are hollow, gas filled proteinaceous nanostructures found in several microbial planktonic microorganisms. They allow positioning of halobacteria at the optimal depth for growth in the poorly aerated, shallow brine pools of their habitat (PubMed:33711860).
Expression of a 9.5 kb p-vac DNA fragment containing 2 divergently transcribed regions (gvpD-gvpE-gvpF-gvpG-gvpH-gvpI-gvpJ-gvpK-gvpL-gvpM and gvpA-gvpC-gvpN-gvpO) allows H.volcanii to produce gas vesicles (PubMed:10894744, PubMed:1404376, PubMed:7651141).
A minimal gas vesicle can be made in H.volcanii by gvpA1-gvpO1 plus gvpF1-gvpG1-gvpJ1-gvpK1-gvpL1-gvpM1; lack of enough GvpJ1 prevents formation (PubMed:10894744).
The same region restores gas vesicle production in H.halobium without the p-vac locus (PubMed:1398080).

Miscellaneous

Encoded in a 14-gene plasmid locus called p-vac which produces predominantly short, spindle-shaped gas vesicles during all stages of growth.

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentgas vesicle
Biological Processgas vesicle organization

Names & Taxonomy

Protein names

  • Recommended name
    Gas vesicle protein F1
  • Short names
    GvpF

Gene names

    • Name
      gvpF11
    • Synonyms
      gvpF
      , p-gvpF
    • Ordered locus names
      VNG_5027G
    • Name
      gvpF1
    • Ordered locus names
      VNG_6026G

Encoded on

  • Plasmid pNRC100
  • Plasmid pNRC200
  • Plasmid pHH1

Organism names

Accessions

  • Primary accession
    Q9HI21
  • Secondary accessions
    • P24370
    • P57731

Proteomes

    • Identifier
    • Component
      Plasmid pNRC100, Plasmid pNRC200

Subcellular Location

Gas vesicle
Cytoplasm
Note: Probably faces the interior of the gas vesicle.

Keywords

Phenotypes & Variants

Disruption phenotype

No gas vesicles are made, gvpA1 is still transcribed.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis3Small gas vesicles.
Mutagenesis14Long, thin gas vesicles.
Mutagenesis15Small gas vesicles.
Mutagenesis19Small gas vesicles.
Mutagenesis65Few, long, thin gas vesicles.
Mutagenesis71No gas vesicles.
Mutagenesis72No gas vesicles.
Mutagenesis213No gas vesicles.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001826811-213Gas vesicle protein F1

Expression

Induction

Part of a gvpF1-gvpG1-gvpH1-gvpI1-gvpJ1-gvpK1-gvpL1-gvpM1 operon, maximally expressed in early to mid log phase (PubMed:1956294, PubMed:7651141).
Detected at very low levels in growing cells, at slightly higher levels in stationary phase (at protein level). Not detected in isolated gas vesicles (PubMed:7651141).
Gas vesicles appear earlier when grown in static culture, possibly due to O2-limitation (PubMed:33711860).

Interaction

Subunit

Binds GvpA1 in early growth stages; is the only one of GvpF1 to GvpM1 that interacts with GvpA1 in H.volcanii experiments (PubMed:33281806, PubMed:34975818).
GvpF to GvpM interact with each other in vitro, and may form multi-subunit complex(es) (PubMed:33281806).
Interacts with GvpC1 and GvpO1 (PubMed:35966690).

Structure

Family & Domains

Sequence similarities

Belongs to the gas vesicle GvpF/GvpL family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    213
  • Mass (Da)
    23,962
  • Last updated
    2001-01-11 v1
  • Checksum
    E0F1936705DB0326
MTENLYTYGIIEQEDLELDVEGVAGAEQVYTVDYKTLSAVVSDIDTTDPERTDEDVEAHNNVLQEVLKHEEERTVVPMSFGMAFKSARTLKGVLRGARRALRSTLNDIEGTVELGVKILGPGDDTVPREEIQENVTDQLADLSINETENDLFTDRLIINKSYLVDFEKRDAFDSAIDDVEAEYDELTIQYTGPWPPYNFVDIHIGAEQQQGGR

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict31in Ref. 2; CAA39173

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M58557
EMBL· GenBank· DDBJ
AAA98193.1
EMBL· GenBank· DDBJ
Genomic DNA
X55648
EMBL· GenBank· DDBJ
CAA39173.1
EMBL· GenBank· DDBJ
Genomic DNA
AF016485
EMBL· GenBank· DDBJ
AAC82806.1
EMBL· GenBank· DDBJ
Genomic DNA
AE004438
EMBL· GenBank· DDBJ
AAG20723.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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