Q9HGZ0 · PFKA2_ASPOR
- ProteinATP-dependent 6-phosphofructokinase 2
- GenepfkB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids775 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H+
Cofactor
Activity regulation
Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 25 | ATP (UniProtKB | ChEBI) | |||
Binding site | 88-89 | ATP (UniProtKB | ChEBI) | |||
Binding site | 118-121 | ATP (UniProtKB | ChEBI) | |||
Binding site | 119 | Mg2+ (UniProtKB | ChEBI); catalytic | |||
Binding site | 164-166 | substrate; ligand shared between dimeric partners; in other chain | |||
Active site | 166 | Proton acceptor | |||
Binding site | 201 | substrate; ligand shared between dimeric partners | |||
Binding site | 208-210 | substrate; ligand shared between dimeric partners; in other chain | |||
Binding site | 264 | substrate; ligand shared between dimeric partners; in other chain | |||
Binding site | 292 | substrate; ligand shared between dimeric partners | |||
Binding site | 298-301 | substrate; ligand shared between dimeric partners; in other chain | |||
Binding site | 537-541 | beta-D-fructose 2,6-bisphosphate (UniProtKB | ChEBI); allosteric activator | |||
Binding site | 582-584 | beta-D-fructose 2,6-bisphosphate (UniProtKB | ChEBI); allosteric activator | |||
Binding site | 640 | beta-D-fructose 2,6-bisphosphate (UniProtKB | ChEBI); allosteric activator | |||
Binding site | 672-675 | beta-D-fructose 2,6-bisphosphate (UniProtKB | ChEBI); allosteric activator | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | 6-phosphofructokinase complex | |
Cellular Component | mitochondrion | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | AMP binding | |
Molecular Function | ATP binding | |
Molecular Function | fructose-6-phosphate binding | |
Molecular Function | identical protein binding | |
Molecular Function | metal ion binding | |
Molecular Function | monosaccharide binding | |
Biological Process | canonical glycolysis | |
Biological Process | fructose 1,6-bisphosphate metabolic process | |
Biological Process | fructose 6-phosphate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent 6-phosphofructokinase 2
- EC number
- Short namesATP-PFK 2 ; Phosphofructokinase 2
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Circumdati
Accessions
- Primary accessionQ9HGZ0
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000112037 | 1-775 | ATP-dependent 6-phosphofructokinase 2 | ||
Interaction
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-390 | N-terminal catalytic PFK domain 1 | |||
Region | 391-404 | Interdomain linker | |||
Region | 405-775 | C-terminal regulatory PFK domain 2 | |||
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length775
- Mass (Da)84,727
- Last updated2001-03-01 v1
- ChecksumB5A78C4F7EE43848
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB032271 EMBL· GenBank· DDBJ | BAB12231.1 EMBL· GenBank· DDBJ | mRNA | ||
BA000056 EMBL· GenBank· DDBJ | BAE66301.1 EMBL· GenBank· DDBJ | Genomic DNA |