Q9HEU0 · TRI14_FUSSP

  • Protein
    Core trichothecene cluster (CTC) protein 14
  • Gene
    TRI14
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    2/5

Function

function

Part of the core gene cluster that mediates the biosynthesis of trichothecenes, a very large family of chemically related bicyclic sesquiterpene compounds acting as mycotoxins, including T2-toxin (PubMed:11352533).
The biosynthesis of trichothecenes begins with the cyclization of farnesyl diphosphate to trichodiene and is catalyzed by the trichodiene synthase TRI5 (PubMed:3800398).
Trichodiene undergoes a series of oxygenations catalyzed by the cytochrome P450 monooxygenase TRI4 (PubMed:7651333).
TRI4 controls the addition of four oxygens at C-2, C-3, C-11, and the C-12, C-13-epoxide to form the intermediate isotrichotriol (PubMed:16917519).
Isotrichotriol then undergoes a non-enzymatic isomerization and cyclization to form isotrichodermol (PubMed:2317042).
During this process, the oxygen at the C-2 position becomes the pyran ring oxygen and the hydroxyl group at C-11 is lost (PubMed:2317042).
More complex type A trichothecenes are built by modifying isotrichodermol through a series of paired hydroxylation and acetylation or acylation steps (PubMed:11352533).
Isotrichodermol is converted to isotrichodermin by the acetyltransferase TRI101 (PubMed:10583973).
TRI101 encodes a C-3 transacetylase that acts as a self-protection or resistance factor during biosynthesis and that the presence of a free C-3 hydroxyl group is a key component of Fusarium trichothecene phytotoxicity (PubMed:10583973).
A second hydroxyl group is added to C-15 by the trichothecene C-15 hydroxylase TRI11, producing 15-decalonectrin, which is then acetylated by TRI3, producing calonectrin (PubMed:8593041, PubMed:9435078).
A third hydroxyl group is added at C-4 by the cytochrome P450 monooxygenase TRI13, converting calonectrin to 3,15-diacetoxyspirpenol, which is subsequently acetylated bythe acetyltransferase TRI7 (PubMed:11352533, PubMed:12135578).
A fourth hydroxyl group is added to C-8 by the cytochrome P450 monooxygenase TRI1, followed by the addition of an isovaleryl moiety by TRI16 (PubMed:12620849, PubMed:14532047).
Finally, the acetyl group is removed from the C-3 position by the trichothecene C-3 esterase TRI8 to produce T-2 toxin (PubMed:12039755).

Names & Taxonomy

Protein names

  • Recommended name
    Core trichothecene cluster (CTC) protein 14

Gene names

    • Name
      TRI14

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 24631 / NRRL 3299
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Nectriaceae > Fusarium

Accessions

  • Primary accession
    Q9HEU0

Phenotypes & Variants

Disruption phenotype

Does not affect the production of trichothecenes (PubMed:12135578).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004423731-373Core trichothecene cluster (CTC) protein 14

Expression

Induction

Expression is positively regulated by the trichothecene cluster-specific transcription activator TRI10 (PubMed:12732543).

Structure

Family & Domains

Sequence similarities

Belongs to the TRI14 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    373
  • Mass (Da)
    41,271
  • Last updated
    2001-03-01 v1
  • Checksum
    9B7F1F0AE8CB5529
MLPQVILNHLGSIGEAASTWFSENKYFGSVGQCPPLPKGDLNYDIYMGYPEMFAWDKKRCVAYVSNLYNATVSTWDPYKSVVLDTIHFPGLSHAGNSASPNPLHASGIILRPDAYHAETLEVVIDNGDAFYSDGFNVSGPDHLMSIDLKTKEVTSQLRLNNGLYAGYADASLGPDGNTYVLGTYSSNILRVTPDKEISTFYVAEPLGPPRLYGFTGIAHVGDAMIVPDNIIGQLIRFDVRDKVGTPVTIKQTPYHEFKTANVLHFPERYNDTILLVAENMTPDYPYGGVSVYQDKTKQFNEVEFLGFLPSRLTNALTTSARQMADRIYVVALPTDGANITVAGESSRFPFQDITEELDLMILPEIKDEARDEI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF326571
EMBL· GenBank· DDBJ
AAG46054.1
EMBL· GenBank· DDBJ
Genomic DNA
AY032746
EMBL· GenBank· DDBJ
AAK77936.1
EMBL· GenBank· DDBJ
mRNA
AF359360
EMBL· GenBank· DDBJ
AAN40835.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp