Q9H0M0 · WWP1_HUMAN
- ProteinNEDD4-like E3 ubiquitin-protein ligase WWP1
- GeneWWP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids922 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Monoubiquitinates AMOTL2 which facilitates its interaction with and activation of LATS2 (PubMed:34404733).
LATS2 then phosphorylates YAP1, excluding it from the nucleus and therefore ultimately represses YAP1-driven transcription of target genes (PubMed:34404733).
Catalytic activity
Activity regulation
Pathway
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 890 | Glycyl thioester intermediate | ||||
Sequence: C | ||||||
Site | 890 | Required for ubiquitin-thioester formation | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | extracellular exosome | |
Cellular Component | nucleus | |
Cellular Component | plasma membrane | |
Cellular Component | ubiquitin ligase complex | |
Molecular Function | ubiquitin protein ligase activity | |
Molecular Function | ubiquitin-protein transferase activity | |
Biological Process | central nervous system development | |
Biological Process | monoatomic ion transmembrane transport | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | proteasome-mediated ubiquitin-dependent protein catabolic process | |
Biological Process | protein ubiquitination | |
Biological Process | signal transduction | |
Biological Process | symbiont entry into host cell |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
The subsequence AMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEEPLPEGWEIRYTREGVR, which contains the WW domain, shows transcriptional activator activity in a high-throughput recruitment assay.
Names & Taxonomy
Protein names
- Recommended nameNEDD4-like E3 ubiquitin-protein ligase WWP1
- EC number
- Alternative names
Gene names
- Community suggested namesWWP1
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ9H0M0
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Keywords
- Cellular component
Disease & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 614 | Reduces ubiquitin transfer. | ||||
Sequence: E → A | ||||||
Mutagenesis | 621 | Strongly reduces ubiquitin transfer. | ||||
Sequence: H → A | ||||||
Mutagenesis | 675 | Reduces ubiquitin transfer. | ||||
Sequence: D → A | ||||||
Mutagenesis | 798 | Reduces ubiquitin transfer. Strongly reduces ubiquitin transfer; when associated with A-845. | ||||
Sequence: E → A | ||||||
Mutagenesis | 804 | Strongly reduces ubiquitin transfer; when associated with P-806. | ||||
Sequence: M → P | ||||||
Mutagenesis | 806 | Strongly reduces ubiquitin transfer; when associated with P-804. | ||||
Sequence: E → P | ||||||
Mutagenesis | 845 | No effect. | ||||
Sequence: R → A | ||||||
Mutagenesis | 848 | Abolishes ubiquitin transfer; when associated with A-855. | ||||
Sequence: Q → A | ||||||
Mutagenesis | 855 | Abolishes ubiquitin transfer; when associated with A-848. | ||||
Sequence: R → A | ||||||
Mutagenesis | 890 | Abolishes monoubiquitination of AMOTL2. | ||||
Sequence: C → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 940 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000120336 | 1-922 | UniProt | NEDD4-like E3 ubiquitin-protein ligase WWP1 | |||
Sequence: MATASPRSDTSNNHSGRLQLQVTVSSAKLKRKKNWFGTAIYTEVVVDGEITKTAKSSSSSNPKWDEQLTVNVTPQTTLEFQVWSHRTLKADALLGKATIDLKQALLIHNRKLERVKEQLKLSLENKNGIAQTGELTVVLDGLVIEQENITNCSSSPTIEIQENGDALHENGEPSARTTARLAVEGTNGIDNHVPTSTLVQNSCCSYVVNGDNTPSSPSQVAARPKNTPAPKPLASEPADDTVNGESSSFAPTDNASVTGTPVVSEENALSPNCTSTTVEDPPVQEILTSSENNECIPSTSAELESEARSILEPDTSNSRSSSAFEAAKSRQPDGCMDPVRQQSGNANTETLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRRRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERGFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE | |||||||
Modified residue (large scale data) | 25 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 26 | PRIDE | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Induction
Gene expression databases
Organism-specific databases
Interaction
Subunit
Interaction with the Crumbs complex promotes WWP1 monoubiquitination of AMOTL2, which activates the Hippo signaling pathway (PubMed:34404733).
Binds KLF2 and HIVEP3 (By similarity).
Binds SCNN1A, SCNN1B, SCNN1G, WBP1, WBP2, DRPLA and adenovirus type 2 PIII. Interacts with RNF11 (By similarity).
Interacts with SPART. Interacts with ERBB4 isoforms JM-B CYT-1 and JM-A CYT-1. Interacts with SMAD1, SMAD2, SMAD3, SMAD5, SMAD6, SMAD7, TGFBR1 and TGFBR2. Associates with the TGFBR1:TGFBR2 receptor complex in presence of SMAD7. Interacts with SKIL isoform 1. Interacts with TP63 isoform 1 and isoform 2. Interacts with STAMBP and RNF11. Interacts with NDFIP1 and NDFIP2 (Probable); this interaction activates the E3 ubiquitin-protein ligase. Interacts with TGIF. Interacts (via WW domains) with ARRDC1, ARRDC2 and ARRDC3 (PubMed:21191027).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9H0M0 | ARRDC1 Q8N5I2 | 5 | EBI-742157, EBI-2339564 | |
BINARY | Q9H0M0 | ARRDC3 Q96B67 | 5 | EBI-742157, EBI-2875665 | |
BINARY | Q9H0M0 | ATXN3 P54252 | 3 | EBI-742157, EBI-946046 | |
BINARY | Q9H0M0 | CPSF6 Q16630 | 6 | EBI-742157, EBI-358410 | |
BINARY | Q9H0M0 | ENTREP1 Q15884 | 3 | EBI-742157, EBI-8636612 | |
BINARY | Q9H0M0 | FBXL18 Q96D16 | 3 | EBI-742157, EBI-744419 | |
BINARY | Q9H0M0 | Q8WU02 | 3 | EBI-742157, EBI-747182 | |
BINARY | Q9H0M0 | SMAD3 P84022 | 9 | EBI-742157, EBI-347161 | |
BINARY | Q9H0M0 | TRAF4 Q9BUZ4 | 3 | EBI-742157, EBI-3650647 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-116 | C2 | ||||
Sequence: MATASPRSDTSNNHSGRLQLQVTVSSAKLKRKKNWFGTAIYTEVVVDGEITKTAKSSSSSNPKWDEQLTVNVTPQTTLEFQVWSHRTLKADALLGKATIDLKQALLIHNRKLERVK | ||||||
Region | 210-388 | Disordered | ||||
Sequence: GDNTPSSPSQVAARPKNTPAPKPLASEPADDTVNGESSSFAPTDNASVTGTPVVSEENALSPNCTSTTVEDPPVQEILTSSENNECIPSTSAELESEARSILEPDTSNSRSSSAFEAAKSRQPDGCMDPVRQQSGNANTETLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWE | ||||||
Compositional bias | 240-327 | Polar residues | ||||
Sequence: DTVNGESSSFAPTDNASVTGTPVVSEENALSPNCTSTTVEDPPVQEILTSSENNECIPSTSAELESEARSILEPDTSNSRSSSAFEAA | ||||||
Compositional bias | 336-354 | Polar residues | ||||
Sequence: MDPVRQQSGNANTETLPSG | ||||||
Domain | 349-382 | WW 1 | ||||
Sequence: ETLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQP | ||||||
Region | 349-531 | Required for interaction with and ubiquitination of AMOTL2. Required for interaction with YAP1 | ||||
Sequence: ETLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRRRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGK | ||||||
Domain | 381-414 | WW 2 | ||||
Sequence: QPLPPGWERRVDDRRRVYYVDHNTRTTTWQRPTM | ||||||
Domain | 456-489 | WW 3 | ||||
Sequence: GPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRT | ||||||
Domain | 496-529 | WW 4 | ||||
Sequence: EPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRN | ||||||
Domain | 588-922 | HECT | ||||
Sequence: KPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE |
Domain
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 6 isoforms produced by Alternative splicing. Additional isoforms seem to exist.
Q9H0M0-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsA
- Length922
- Mass (Da)105,202
- Last updated2001-03-01 v1
- Checksum35B6E1C03A3147DA
Q9H0M0-2
- Name2
- SynonymsB
Q9H0M0-3
- Name3
- SynonymsC
- Differences from canonical
- 112-242: LERVKEQLKLSLENKNGIAQTGELTVVLDGLVIEQENITNCSSSPTIEIQENGDALHENGEPSARTTARLAVEGTNGIDNHVPTSTLVQNSCCSYVVNGDNTPSSPSQVAARPKNTPAPKPLASEPADDTV → F
Q9H0M0-4
- Name4
- SynonymsD
Q9H0M0-5
- Name5
- SynonymsE
Q9H0M0-6
- Name6
- SynonymsF
- Differences from canonical
- 23-240: Missing
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_007600 | 23-240 | in isoform 6 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_007601 | 112-120 | in isoform 2 | |||
Sequence: LERVKEQLK → CWLLKARME | ||||||
Alternative sequence | VSP_007602 | 112-242 | in isoform 3 | |||
Sequence: LERVKEQLKLSLENKNGIAQTGELTVVLDGLVIEQENITNCSSSPTIEIQENGDALHENGEPSARTTARLAVEGTNGIDNHVPTSTLVQNSCCSYVVNGDNTPSSPSQVAARPKNTPAPKPLASEPADDTV → F | ||||||
Alternative sequence | VSP_007603 | 121-922 | in isoform 2 | |||
Sequence: Missing | ||||||
Compositional bias | 240-327 | Polar residues | ||||
Sequence: DTVNGESSSFAPTDNASVTGTPVVSEENALSPNCTSTTVEDPPVQEILTSSENNECIPSTSAELESEARSILEPDTSNSRSSSAFEAA | ||||||
Compositional bias | 336-354 | Polar residues | ||||
Sequence: MDPVRQQSGNANTETLPSG |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL136739 EMBL· GenBank· DDBJ | CAB66673.1 EMBL· GenBank· DDBJ | mRNA | ||
AY043361 EMBL· GenBank· DDBJ | AAK94668.1 EMBL· GenBank· DDBJ | mRNA | ||
AY345857 EMBL· GenBank· DDBJ | AAQ22764.1 EMBL· GenBank· DDBJ | mRNA | ||
AC083845 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC103817 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC015380 EMBL· GenBank· DDBJ | AAH15380.2 EMBL· GenBank· DDBJ | mRNA | ||
BC036065 EMBL· GenBank· DDBJ | AAH36065.1 EMBL· GenBank· DDBJ | mRNA | ||
U96113 EMBL· GenBank· DDBJ | AAC51324.1 EMBL· GenBank· DDBJ | mRNA |