Q9GV45 · LUCI_OPLGR
- ProteinOplophorus-luciferin 2-monooxygenase catalytic subunit
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids196 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalytic subunit of oplophorus-luciferin 2-monooxygenase. Oxidoreductase that converts coelenterazine (the oplophorus luciferin) to coelenteramide under emission of blue light with a maximum at 454 nm. Is also active with bisdeoxycoelenterazine.
Miscellaneous
The shrimp has luminous glands at the base of its antennae and legs, and ejects a cloud of brightly luminescent secretion from the base of its antennae upon stimulation.
Catalytic activity
- coelenterazine + O2 = coelenteramide + hnu + CO2
Activity regulation
Inhibited by micromolar Cu2+.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
3.73 μM | coelenterazine | |||||
1.3 μM | bis-coelenterazine |
pH Dependence
Optimum pH is 8-9.
Temperature Dependence
Optimum temperature is 40 degrees Celsius.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | Oplophorus-luciferin 2-monooxygenase activity | |
Biological Process | bioluminescence |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameOplophorus-luciferin 2-monooxygenase catalytic subunit
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Crustacea > Multicrustacea > Malacostraca > Eumalacostraca > Eucarida > Decapoda > Pleocyemata > Caridea > Oplophoroidea > Oplophoridae > Oplophorus
Accessions
- Primary accessionQ9GV45
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-27 | |||||
Sequence: MAYSTLFIIALTAVVTQASSTQKSNLT | ||||||
Chain | PRO_0000418820 | 28-196 | Oplophorus-luciferin 2-monooxygenase catalytic subunit | |||
Sequence: FTLADFVGDWQQTAGYNQDQVLEQGGLSSLFQALGVSVTPIQKVVLSGENGLKADIHVIIPYEGLSGFQMGLIEMIFKVVYPVDDHHFKIILHYGTLVIDGVTPNMIDYFGRPYPGIAVFDGKQITVTGTLWNGNKIYDERLINPDGSLLFRVTINGVTGWRLCENILA |
Interaction
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length196
- Mass (Da)21,533
- Last updated2001-03-01 v1
- Checksum056DC0753F0B8CCF
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB030246 EMBL· GenBank· DDBJ | BAB13776.1 EMBL· GenBank· DDBJ | mRNA |