Q9GUM2 · BRE4_CAEEL

  • Protein
    Beta-1,4-N-acetylgalactosaminyltransferase bre-4
  • Gene
    bre-4
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the transfer of galactose onto proteins or lipids. Required for susceptibility to pore-forming crystal toxins in conjunction with bre-1, bre-2, bre-3 and bre-5.

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Protein modification; protein glycosylation.

Features

Showing features for binding site.

138350100150200250300350
TypeIDPosition(s)Description
Binding site222Mn2+ (UniProtKB | ChEBI)
Binding site315Mn2+ (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular ComponentGolgi apparatus
Cellular Componentmembrane
Molecular Functionacetylgalactosaminyltransferase activity
Molecular Functionbeta-1,4-N-acetylgalactosaminyltransferase activity
Molecular Functiongalactosyltransferase activity
Molecular Functionmetal ion binding
Molecular FunctionN-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase activity
Biological Processcarbohydrate metabolic process
Biological Processglycosphingolipid biosynthetic process
Biological Processpositive regulation of Notch signaling pathway
Biological Processprotein glycosylation
Biological Processresponse to toxic substance

Keywords

Enzyme and pathway databases

Protein family/group databases

    • GT7Glycosyltransferase Family 7

Names & Taxonomy

Protein names

  • Recommended name
    Beta-1,4-N-acetylgalactosaminyltransferase bre-4
  • EC number
  • Alternative names
    • Bacillus thuringiensis toxin-resistant protein 4 (Bt toxin-resistant protein 4)
    • Beta-4-GalNAcT

Gene names

    • Name
      bre-4
    • ORF names
      Y73E7A.7

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q9GUM2
  • Secondary accessions
    • W6SB74

Proteomes

Organism-specific databases

Subcellular Location

Membrane
; Single-pass type II membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-6Cytoplasmic
Transmembrane7-29Helical; Signal-anchor for type II membrane protein
Topological domain30-383Extracellular

Keywords

Phenotypes & Variants

Disruption phenotype

Worms exhibit resistance to the Cry5B toxin produced by Bacillus thuringiensis. This is thought to be due to mutants having reduced population of glycolipids which are targeted by the Cry5B protein.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis260In ye43; resistant to Bacillus thuringiensis crystal5B toxin.

PTM/Processing

Features

Showing features for chain, glycosylation, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00003246711-383Beta-1,4-N-acetylgalactosaminyltransferase bre-4
Glycosylation37N-linked (GlcNAc...) asparagine
Glycosylation71N-linked (GlcNAc...) asparagine
Glycosylation81N-linked (GlcNAc...) asparagine
Glycosylation97N-linked (GlcNAc...) asparagine
Disulfide bond103↔144
Glycosylation191N-linked (GlcNAc...) asparagine
Disulfide bond215↔234

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the glycosyltransferase 7 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q9GUM2-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    383
  • Mass (Da)
    43,914
  • Last updated
    2001-03-01 v1
  • Checksum
    ADEDFA4275B2CE81
MAFRHLAVARLKSLLVLCAVLLLVHAMIYKIPSLYENLTIGSSTLIADVDAMEAVLGNTASTSDDLLDTWNSTFSPISEVNQTSFMEDIRPILFPDNQTLQFCNQTPPHLVGPIRVFLDEPDFKTLEKIYPDTHAGGHGMPKDCVARHRVAIIVPYRDREAHLRIMLHNLHSLLAKQQLDYAIFIVEQVANQTFNRGKLMNVGYDVASRLYPWQCFIFHDVDLLPEDDRNLYTCPIQPRHMSVAIDKFNYKLPYSAIFGGISALTKDHLKKINGFSNDFWGWGGEDDDLATRTSMAGLKVSRYPTQIARYKMIKHSTEATNPVNKCRYKIMGQTKRRWTRDGLSNLKYKLVNLELKPLYTRAVVDLLEKDCRRELRRDFPTCF

Q9GUM2-2

  • Name
    b
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0549651-165in isoform b

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY130767
EMBL· GenBank· DDBJ
AAM95168.1
EMBL· GenBank· DDBJ
mRNA
AY533306
EMBL· GenBank· DDBJ
AAS21308.1
EMBL· GenBank· DDBJ
mRNA
FO081821
EMBL· GenBank· DDBJ
CCD73531.1
EMBL· GenBank· DDBJ
Genomic DNA
FO081821
EMBL· GenBank· DDBJ
CDM63463.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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