Q9GLF5 · NKG2D_PIG

  • Protein
    NKG2-D type II integral membrane protein
  • Gene
    KLRK1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    4/5

Function

function

Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8+ T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins (By similarity).

Miscellaneous

Is not capable of signal transduction by itself, but operates through the adapter protein HCST.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell surface
Cellular Componentexternal side of plasma membrane
Molecular Functioncarbohydrate binding
Molecular Functionsignaling receptor activity
Biological Processadaptive immune response
Biological Processcell differentiation
Biological Processinnate immune response
Biological Processpositive regulation of natural killer cell mediated cytotoxicity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    NKG2-D type II integral membrane protein
  • Alternative names
    • Killer cell lectin-like receptor subfamily K member 1
    • NK cell receptor D
    • NKG2-D-activating NK receptor
  • CD Antigen Name
    • CD314

Gene names

    • Name
      KLRK1
    • Synonyms
      NKG2D

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Suina > Suidae > Sus

Accessions

  • Primary accession
    Q9GLF5
  • Secondary accessions
    • Q9GL58

Proteomes

Subcellular Location

Cell membrane
; Single-pass type II membrane protein
Note: Colocalized with HCST on the cell surface.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-56Cytoplasmic
Transmembrane57-77Helical; Signal-anchor for type II membrane protein
Topological domain78-214Extracellular

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00002745591-214NKG2-D type II integral membrane protein
Disulfide bond94↔103
Disulfide bond97↔108
Glycosylation113N-linked (GlcNAc...) asparagine
Disulfide bond125↔209
Glycosylation129N-linked (GlcNAc...) asparagine
Glycosylation161N-linked (GlcNAc...) asparagine
Glycosylation184N-linked (GlcNAc...) asparagine
Disulfide bond187↔201

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Detected in peripheral blood leukocytes, macrophages, monocytes and natural killer cells.

Interaction

Subunit

Homodimer; disulfide-linked. Heterohexamer composed of two subunits of KLRK1 and four subunits of HCST/DAP10. Interacts (via transmembrane domain) with HCST/DAP10 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface and induces NK cell-mediated cytotoxicity. Can form disulfide-bonded heterodimer with CD94 (By similarity).
Interacts with CEACAM1; recruits PTPN6 that dephosphorylates VAV1 (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain98-210C-type lectin

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q9GLF5-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    214
  • Mass (Da)
    24,773
  • Last updated
    2001-03-01 v1
  • Checksum
    22C0857BBE136362
MGWIRDRRSPSSMEIRELHNRDVINRGAFKSRQKRTQTLITSKCGENPSPFFLARSIAIAMGIRFIVMVMIYSGMIINLLFNQEAPSPLKESYCGPCPKNWICYRNSCYQFSNESKTWLQSQASCRSQNSSLLKIYSREDQDFFKLVKSYHWMGLVQIPTNRSWQWEDGSILSPNQITMVEMQNGSCAVYGSSFKGYTENCLTLNTYICMKRTV

Q9GLF5-2

  • Name
    2
  • Synonyms
    Truncated
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Features

Showing features for sequence conflict, alternative sequence.

TypeIDPosition(s)Description
Sequence conflict6in Ref. 1; AAG33631
Alternative sequenceVSP_02279379-80in isoform 2
Alternative sequenceVSP_02279481-214in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF285448
EMBL· GenBank· DDBJ
AAG29426.1
EMBL· GenBank· DDBJ
mRNA
AF313487
EMBL· GenBank· DDBJ
AAG33631.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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