Q9FZ97 · MUC70_ARATH
- ProteinProbable hexosyltransferase MUCI70
- GeneMUCI70
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids581 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Probable glycosyltransferase involved in pectin and/or xylans biosynthesis in cell walls (By similarity).
Together with IRX14, required for xylan and pectin synthesis in seed coat epidermal (SCE) cells (PubMed:30228108).
Collaboratively with GAUT11, essential for the accumulation of seed mucilage, a gelatinous wall rich in unbranched rhamnogalacturonan I (RG I), and for shaping the surface morphology of seeds (PubMed:30228108).
Together with IRX14, required for xylan and pectin synthesis in seed coat epidermal (SCE) cells (PubMed:30228108).
Collaboratively with GAUT11, essential for the accumulation of seed mucilage, a gelatinous wall rich in unbranched rhamnogalacturonan I (RG I), and for shaping the surface morphology of seeds (PubMed:30228108).
Pathway
Glycan metabolism; pectin biosynthesis.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi membrane | |
Molecular Function | glycosyltransferase activity | |
Biological Process | mucilage extrusion from seed coat | |
Biological Process | mucilage pectin biosynthetic process | |
Biological Process | rhamnogalacturonan I biosynthetic process | |
Biological Process | xylan metabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable hexosyltransferase MUCI70
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9FZ97
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-58 | Cytoplasmic | |||
Transmembrane | 59-79 | Helical; Signal-anchor for type II membrane protein | |||
Topological domain | 80-581 | Lumenal | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Strong reduction of seed mucilage accumulation and major decrease in rhamnogalacturonan I (RG I), associated with reduced absolute levels of rhamnose (Rha), arabinose (Ara) and galacturonic acid (GalA) but increased absolute abundance of minor sugars (e.g. galactose (Gal), xylose (Xyl), glucose (Glc) and mannose (Man)) (PubMed:30228108).
The double mutant muci70-1 gaut11-3 is completely defective in seed mucilage production and exhibits a strong release of minor sugars in total mucilage extracts (PubMed:30228108).
Plants missing both MUCI70 and IRX14 exhibit a severe reduction in both xylan- and pectin-related sugars in total seed mucilage extracts (PubMed:30228108).
The double mutant muci70-1 gaut11-3 is completely defective in seed mucilage production and exhibits a strong release of minor sugars in total mucilage extracts (PubMed:30228108).
Plants missing both MUCI70 and IRX14 exhibit a severe reduction in both xylan- and pectin-related sugars in total seed mucilage extracts (PubMed:30228108).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 31 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000450758 | 1-581 | Probable hexosyltransferase MUCI70 | ||
Glycosylation | 96 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 102 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 119 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 194 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 224 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 285 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 382 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 411 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 488 | N-linked (GlcNAc...) asparagine | |||
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in siliques and seeds.
Developmental stage
Accumulates in developing seeds in siliques, mainly in the seed coat and, to a lesser extent, in the embryo.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 514-581 | Disordered | |||
Compositional bias | 520-539 | Pro residues | |||
Compositional bias | 550-569 | Basic residues | |||
Sequence similarities
Belongs to the glycosyltransferase 8 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length581
- Mass (Da)65,578
- Last updated2001-03-01 v1
- Checksum78E72252332F91AC
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 177 | in Ref. 1; ARJ31408 and 4; AAL32582/AAN72102 | |||
Compositional bias | 520-539 | Pro residues | |||
Compositional bias | 550-569 | Basic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KY906044 EMBL· GenBank· DDBJ | ARJ31408.1 EMBL· GenBank· DDBJ | mRNA | ||
AC021044 EMBL· GenBank· DDBJ | AAF98431.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE30935.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY062504 EMBL· GenBank· DDBJ | AAL32582.1 EMBL· GenBank· DDBJ | mRNA | ||
BT002091 EMBL· GenBank· DDBJ | AAN72102.1 EMBL· GenBank· DDBJ | mRNA |