Q9FYE1 · MCA9_ARATH
- ProteinMetacaspase-9
- GeneAMC9
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids325 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Cysteine protease that cleaves specifically after arginine or lysine residues. Does not cleave caspase-specific substrates. Required for proteolytic processing of GRI (PubMed:25398910).
Activity regulation
Inhibited by serpin ZX and nitric oxide through cysteine nitrosylation.
Features
Showing features for site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 29 | S-nitrosylation-insensitive cysteine; may replace the S-nitrosylated cysteine residue within the catalytic center (in mature processed form only) | ||||
Sequence: C | ||||||
Active site | 95 | |||||
Sequence: H | ||||||
Active site | 147 | |||||
Sequence: C | ||||||
Site | 183-184 | Cleavage; by autolysis | ||||
Sequence: RA |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | apoplast | |
Molecular Function | cysteine-type endopeptidase activity | |
Molecular Function | cysteine-type peptidase activity | |
Biological Process | proteolysis |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameMetacaspase-9
- EC number
- Short namesAtMC9
- Cleaved into 2 chains
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9FYE1
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 29 | Reduced proteolytic activity. | ||||
Sequence: C → A | ||||||
Mutagenesis | 147 | Loss of autoprocessing and protease activity. | ||||
Sequence: C → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 25 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000334608 | 1-183 | Metacaspase-9 subunit p20 | |||
Sequence: MDQQGMVKKRLAVLVGCNYPNTRNELHGCINDVLAMKETILSRFGFKQDDIEVLTDEPESKVKPTGANIKAALRRMVDKAQAGSGDILFFHYSGHGTRIPSVKSAHPFKQDEAIVPCDFNLITDVDFRELVNQLPKGTSFTMISDSCHSGGLIDKEKEQIGPSSVSSNISPAIETTNKTITSR | ||||||
Chain | PRO_0000334607 | 1-325 | Metacaspase-9 | |||
Sequence: MDQQGMVKKRLAVLVGCNYPNTRNELHGCINDVLAMKETILSRFGFKQDDIEVLTDEPESKVKPTGANIKAALRRMVDKAQAGSGDILFFHYSGHGTRIPSVKSAHPFKQDEAIVPCDFNLITDVDFRELVNQLPKGTSFTMISDSCHSGGLIDKEKEQIGPSSVSSNISPAIETTNKTITSRALPFKAVLDHLSSLTGITTSDIGTHLLELFGRDAGLKFRLPAMDLMDLLETMTAREKHVDSGILMSGCQADETSADVGVGNGKAYGAFSNAIQRVLNENEGAMKNKQLVMMARDVLERLGFHQHPCLYCSDQNADATFLSQP | ||||||
Modified residue | 147 | S-nitrosocysteine | ||||
Sequence: C | ||||||
Glycosylation | 177 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Chain | PRO_0000334609 | 184-325 | Metacaspase-9 subunit p10 | |||
Sequence: ALPFKAVLDHLSSLTGITTSDIGTHLLELFGRDAGLKFRLPAMDLMDLLETMTAREKHVDSGILMSGCQADETSADVGVGNGKAYGAFSNAIQRVLNENEGAMKNKQLVMMARDVLERLGFHQHPCLYCSDQNADATFLSQP |
Post-translational modification
The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
S-nitrosylation at Cys-147 suppresses both autoprocessing and proteolytic activity of the full-length protein, but does not affect the activity of the mature processed form.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in root tips, cauline leaves, flowers and siliques.
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length325
- Mass (Da)35,505
- Last updated2001-03-01 v1
- ChecksumAB574D648EDF3E0E
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY219834 EMBL· GenBank· DDBJ | AAP44522.1 EMBL· GenBank· DDBJ | mRNA | ||
AY322531 EMBL· GenBank· DDBJ | AAP84712.1 EMBL· GenBank· DDBJ | mRNA | ||
AL391716 EMBL· GenBank· DDBJ | CAC05502.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED90710.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY063830 EMBL· GenBank· DDBJ | AAL36186.1 EMBL· GenBank· DDBJ | mRNA | ||
AY091308 EMBL· GenBank· DDBJ | AAM14247.1 EMBL· GenBank· DDBJ | mRNA | ||
AY086438 EMBL· GenBank· DDBJ | AAM63441.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |