Q9FKA7 · ORTH1_ARATH
- ProteinE3 ubiquitin-protein ligase ORTHRUS 1
- GeneORTH1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids617 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
E3 ubiquitin-protein ligase. Participates in CpG methylation-dependent transcriptional regulation and epigenetic transcriptional silencing. Mediates ubiquitination with the E2 ubiquitin-conjugating enzymes UBC11, UBC8 and UBC8 homologs (e.g. UBC10, UBC11, UBC28 and UBC29) but not with UBC27, UBC30, UBC32, UBC34 and UBC36. Promotes methylation-mediated gene silencing leading, for example, to early flowering. Can bind to CpG, CpNpG, and CpNpN DNA motifs, with a strong preference for methylated forms, and with highest affinity for CpG substrate.
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | histone binding | |
Molecular Function | metal ion binding | |
Molecular Function | methyl-CpG binding | |
Molecular Function | methyl-CpNpG binding | |
Molecular Function | methyl-CpNpN binding | |
Molecular Function | ubiquitin-protein transferase activity | |
Biological Process | negative regulation of gene expression via chromosomal CpG island methylation | |
Biological Process | protein ubiquitination | |
Biological Process | vegetative to reproductive phase transition of meristem |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameE3 ubiquitin-protein ligase ORTHRUS 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9FKA7
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Broadly distributed in the nucleus and enriched in the heterochromatic chromocenters.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Decreased DNA methylation primarily at CpG sites in genic regions, as well as repeated sequences in heterochromatic regions. Released transcriptional silencing at heterochromatin regions. Ectopic CpHpH methylation in the 5S rRNA genes against a background of CpG hypomethylation.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 292 | Loss of DNA binding activity. | |||
Mutagenesis | 362 | Loss of DNA binding activity. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 61 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000396825 | 1-617 | E3 ubiquitin-protein ligase ORTHRUS 1 | ||
Proteomic databases
PTM databases
Expression
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for zinc finger, domain, coiled coil, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Zinc finger | 12-62 | PHD-type | |||
Zinc finger | 129-169 | RING-type 1 | |||
Domain | 258-407 | YDG | |||
Zinc finger | 495-552 | RING-type 2 | |||
Coiled coil | 563-593 | ||||
Region | 575-617 | Disordered | |||
Domain
The RING fingers are required for ubiquitin ligase activity.
The YDG domain mediates the interaction with histone H3.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length617
- Mass (Da)68,299
- Last updated2001-03-01 v1
- Checksum1CAA1ECD2750A165
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 439 | in Ref. 4; BAD44541 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB012243 EMBL· GenBank· DDBJ | BAB08886.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED94446.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT010573 EMBL· GenBank· DDBJ | AAQ65196.1 EMBL· GenBank· DDBJ | mRNA | ||
AK176778 EMBL· GenBank· DDBJ | BAD44541.1 EMBL· GenBank· DDBJ | mRNA | ||
AK221256 EMBL· GenBank· DDBJ | BAD93904.1 EMBL· GenBank· DDBJ | mRNA |