Q9FKA7 · ORTH1_ARATH

Function

function

E3 ubiquitin-protein ligase. Participates in CpG methylation-dependent transcriptional regulation and epigenetic transcriptional silencing. Mediates ubiquitination with the E2 ubiquitin-conjugating enzymes UBC11, UBC8 and UBC8 homologs (e.g. UBC10, UBC11, UBC28 and UBC29) but not with UBC27, UBC30, UBC32, UBC34 and UBC36. Promotes methylation-mediated gene silencing leading, for example, to early flowering. Can bind to CpG, CpNpG, and CpNpN DNA motifs, with a strong preference for methylated forms, and with highest affinity for CpG substrate.

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentnucleus
Molecular Functionhistone binding
Molecular Functionmetal ion binding
Molecular Functionmethyl-CpG binding
Molecular Functionmethyl-CpNpG binding
Molecular Functionmethyl-CpNpN binding
Molecular Functionubiquitin-protein transferase activity
Biological Processnegative regulation of gene expression via chromosomal CpG island methylation
Biological Processprotein ubiquitination
Biological Processvegetative to reproductive phase transition of meristem

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    E3 ubiquitin-protein ligase ORTHRUS 1
  • EC number
  • Alternative names
    • Protein VARIANT IN METHYLATION 3
    • RING-type E3 ubiquitin transferase ORTHRUS 1

Gene names

    • Name
      ORTH1
    • Synonyms
      VIM3
    • ORF names
      MIJ24.3
    • Ordered locus names
      At5g39550

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9FKA7
  • Secondary accessions
    • Q67XP0

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Nucleus
Note: Broadly distributed in the nucleus and enriched in the heterochromatic chromocenters.

Keywords

Phenotypes & Variants

Disruption phenotype

Decreased DNA methylation primarily at CpG sites in genic regions, as well as repeated sequences in heterochromatic regions. Released transcriptional silencing at heterochromatin regions. Ectopic CpHpH methylation in the 5S rRNA genes against a background of CpG hypomethylation.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis292Loss of DNA binding activity.
Mutagenesis362Loss of DNA binding activity.

Variants

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The viewer provides 61 variants from UniProt as well as other sources including ClinVar and dbSNP.

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PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003968251-617E3 ubiquitin-protein ligase ORTHRUS 1

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in inflorescences and leaves.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for zinc finger, domain, coiled coil, region.

Type
IDPosition(s)Description
Zinc finger12-62PHD-type
Zinc finger129-169RING-type 1
Domain258-407YDG
Zinc finger495-552RING-type 2
Coiled coil563-593
Region575-617Disordered

Domain

The RING fingers are required for ubiquitin ligase activity.
The YDG domain mediates the interaction with histone H3.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    617
  • Mass (Da)
    68,299
  • Last updated
    2001-03-01 v1
  • Checksum
    1CAA1ECD2750A165
MAIETQLPCDGDGVCMRCQVNPPSEETLTCGTCVTPWHVPCLLPESLASSTGEWECPDCSGVVVPSAAPGTGNARPESSGSVLVAAIRAIQADETLTEAEKAKKRQKLMSGGGDDGVDEEEKKKLEIFCSICIQLPERPITTPCGHNFCLKCFEKWAVGQGKLTCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSVEATAAKVHHIIRNQDRPEKAFTTERAVKTGKANAASGKFFVTIPRDHFGPIPAENDVTRKQGVLVGESWEDRQECRQWGAHFPHIAGIAGQSAVGAQSVALSGGYDDDEDHGEWFLYTGSGGRDLSGNKRINKKQSSDQAFKNMNESLRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDGVYRIEKCWSNVGVQGSFKVCRYLFVRCDNEPAPWTSDEHGDRPRPLPNVPELETAADLFVRKESPSWDFDEAEGRWKWMKSPPVSRMALDPEERKKNKRAKNTMKARLLKEFSCQICREVLSLPVTTPCAHNFCKACLEAKFAGITQLRERSNGGRKLRAKKNIMTCPCCTTDLSEFLQNPQVNREMMEIIENFKKSEEEADASISEEEEEESEPPTKKIKMDNNSVGGSGTSLSA

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict439in Ref. 4; BAD44541

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB012243
EMBL· GenBank· DDBJ
BAB08886.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED94446.1
EMBL· GenBank· DDBJ
Genomic DNA
BT010573
EMBL· GenBank· DDBJ
AAQ65196.1
EMBL· GenBank· DDBJ
mRNA
AK176778
EMBL· GenBank· DDBJ
BAD44541.1
EMBL· GenBank· DDBJ
mRNA
AK221256
EMBL· GenBank· DDBJ
BAD93904.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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