Q9FGQ6 · EB1C_ARATH

Function

function

Plant-specific EB1 subtype that functions preferentially at early stages of plant mitosis by regulating spindle positioning and chromosome segregation. Accumulates in the prophase nucleus and is required to maintain spindle bipolarity during premetaphase and/or metaphase and for efficient segregation of chromosomes at anaphase. May play a role in the dynamics of microtubule network in elongating pollen tubes.

Miscellaneous

Plant microtubules behave differently from those of other eukaryotes in mitosis: they lack centrosomes and spindles are barrel-shaped with unfocused poles and no astral microtubules.

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentmicrotubule
Cellular Componentnucleolus
Cellular Componentnucleus
Cellular Componentphragmoplast
Cellular Componentspindle
Molecular Functionmicrotubule binding
Biological Processcell division
Biological Processcortical cytoskeleton organization
Biological Processthigmotropism

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Microtubule-associated protein RP/EB family member 1C
  • Alternative names
    • APC-binding protein EB1C
    • End-binding protein 1C (AtEB1C)
    • Protein ATEB1 homolog 1 (AtEB1H1)

Gene names

    • Name
      EB1C
    • ORF names
      K3G17.3
    • Ordered locus names
      At5g67270

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9FGQ6

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Note: During mitosis, accumulates in the prophase nucleus, and after the nuclear envelope disintegration is associated with whole spindle microtubules, plus end of microtubules, phragmoplast and finally is actively recruited to the nucleus. Localizes in the microtubule network in elongating pollen tubes.

Keywords

Phenotypes & Variants

Disruption phenotype

No visible phenotype under normal growth conditions, but seedlings show increased sensitivity to oryzalin, a microtubule-destabilizing agent. conditions.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis289-291Loss of targeting to nucleus.
Mutagenesis309-311Loss of targeting to nucleus.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 13 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004184121-329Microtubule-associated protein RP/EB family member 1C

Proteomic databases

PTM databases

Expression

Tissue specificity

Highly expressed in the root and shoot meristems, in guard cells of leaf stomata, pollen grains and pollen tubes.

Gene expression databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain13-115Calponin-homology (CH)
Region130-203Disordered
Compositional bias142-197Polar residues
Domain193-263EB1 C-terminal
Region289-311Required for nuclear localization

Domain

Composed of two functionally independent domains. The N-terminal domain forms a hydrophobic cleft involved in microtubule binding and the C-terminal is involved in protein binding. In Arabidopsis thaliana, EB1A and EB1B possess an acidic C-terminal tail that has autoinhibitory function, but EB1C has a tail region with patches of basic amino acid residues required for nuclear targeting.

Sequence similarities

Belongs to the MAPRE family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    329
  • Mass (Da)
    36,386
  • Last updated
    2001-03-01 v1
  • Checksum
    27115C5E35F619E2
MATNIGMMDSAYFVGRSEILAWINSTLQLNLSKVEEACSGAVHCQLMDSVHPGTVPMHKVNFDAKSEYEMIQNYKVLQDVFNKLKITKHIEVSKLVKGRPLDNLEFMQWMKKYCDSVNGGQHNYHALERREASKGGKEATKRAAATQQSGKSSSSSAPPRPSSSNGTRKHEPQSNNTGTHHSSTGNHHHSSKPSAKQSKPVPAYDEKITELKLYIDSLEKERDFYFSKLRDVEILCQNPDTEHLPLVGSIKRILYAADGEDVGAAETQTLSPIAEGSEERRNSVTESQKRKLIVNLDVDVAAITTLSPRQRLSDASDVKCSGSSPLLTC

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias142-197Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB025614
EMBL· GenBank· DDBJ
BAB09646.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED98323.1
EMBL· GenBank· DDBJ
Genomic DNA
AK175495
EMBL· GenBank· DDBJ
BAD43258.1
EMBL· GenBank· DDBJ
mRNA
BT028930
EMBL· GenBank· DDBJ
ABI49477.1
EMBL· GenBank· DDBJ
mRNA
AY087775
EMBL· GenBank· DDBJ
AAM65311.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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