Q9FGQ6 · EB1C_ARATH
- ProteinMicrotubule-associated protein RP/EB family member 1C
- GeneEB1C
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids329 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plant-specific EB1 subtype that functions preferentially at early stages of plant mitosis by regulating spindle positioning and chromosome segregation. Accumulates in the prophase nucleus and is required to maintain spindle bipolarity during premetaphase and/or metaphase and for efficient segregation of chromosomes at anaphase. May play a role in the dynamics of microtubule network in elongating pollen tubes.
Miscellaneous
Plant microtubules behave differently from those of other eukaryotes in mitosis: they lack centrosomes and spindles are barrel-shaped with unfocused poles and no astral microtubules.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | microtubule | |
Cellular Component | nucleolus | |
Cellular Component | nucleus | |
Cellular Component | phragmoplast | |
Cellular Component | spindle | |
Molecular Function | microtubule binding | |
Biological Process | cell division | |
Biological Process | cortical cytoskeleton organization | |
Biological Process | thigmotropism |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameMicrotubule-associated protein RP/EB family member 1C
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9FGQ6
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: During mitosis, accumulates in the prophase nucleus, and after the nuclear envelope disintegration is associated with whole spindle microtubules, plus end of microtubules, phragmoplast and finally is actively recruited to the nucleus. Localizes in the microtubule network in elongating pollen tubes.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No visible phenotype under normal growth conditions, but seedlings show increased sensitivity to oryzalin, a microtubule-destabilizing agent. conditions.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 289-291 | Loss of targeting to nucleus. | ||||
Sequence: KRK → AAA | ||||||
Mutagenesis | 309-311 | Loss of targeting to nucleus. | ||||
Sequence: RQR → AQA |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 13 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000418412 | 1-329 | Microtubule-associated protein RP/EB family member 1C | |||
Sequence: MATNIGMMDSAYFVGRSEILAWINSTLQLNLSKVEEACSGAVHCQLMDSVHPGTVPMHKVNFDAKSEYEMIQNYKVLQDVFNKLKITKHIEVSKLVKGRPLDNLEFMQWMKKYCDSVNGGQHNYHALERREASKGGKEATKRAAATQQSGKSSSSSAPPRPSSSNGTRKHEPQSNNTGTHHSSTGNHHHSSKPSAKQSKPVPAYDEKITELKLYIDSLEKERDFYFSKLRDVEILCQNPDTEHLPLVGSIKRILYAADGEDVGAAETQTLSPIAEGSEERRNSVTESQKRKLIVNLDVDVAAITTLSPRQRLSDASDVKCSGSSPLLTC |
Proteomic databases
PTM databases
Expression
Tissue specificity
Highly expressed in the root and shoot meristems, in guard cells of leaf stomata, pollen grains and pollen tubes.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 13-115 | Calponin-homology (CH) | ||||
Sequence: FVGRSEILAWINSTLQLNLSKVEEACSGAVHCQLMDSVHPGTVPMHKVNFDAKSEYEMIQNYKVLQDVFNKLKITKHIEVSKLVKGRPLDNLEFMQWMKKYCD | ||||||
Region | 130-203 | Disordered | ||||
Sequence: REASKGGKEATKRAAATQQSGKSSSSSAPPRPSSSNGTRKHEPQSNNTGTHHSSTGNHHHSSKPSAKQSKPVPA | ||||||
Compositional bias | 142-197 | Polar residues | ||||
Sequence: RAAATQQSGKSSSSSAPPRPSSSNGTRKHEPQSNNTGTHHSSTGNHHHSSKPSAKQ | ||||||
Domain | 193-263 | EB1 C-terminal | ||||
Sequence: PSAKQSKPVPAYDEKITELKLYIDSLEKERDFYFSKLRDVEILCQNPDTEHLPLVGSIKRILYAADGEDVG | ||||||
Region | 289-311 | Required for nuclear localization | ||||
Sequence: KRKLIVNLDVDVAAITTLSPRQR |
Domain
Composed of two functionally independent domains. The N-terminal domain forms a hydrophobic cleft involved in microtubule binding and the C-terminal is involved in protein binding. In Arabidopsis thaliana, EB1A and EB1B possess an acidic C-terminal tail that has autoinhibitory function, but EB1C has a tail region with patches of basic amino acid residues required for nuclear targeting.
Sequence similarities
Belongs to the MAPRE family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length329
- Mass (Da)36,386
- Last updated2001-03-01 v1
- Checksum27115C5E35F619E2
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 142-197 | Polar residues | ||||
Sequence: RAAATQQSGKSSSSSAPPRPSSSNGTRKHEPQSNNTGTHHSSTGNHHHSSKPSAKQ |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB025614 EMBL· GenBank· DDBJ | BAB09646.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED98323.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK175495 EMBL· GenBank· DDBJ | BAD43258.1 EMBL· GenBank· DDBJ | mRNA | ||
BT028930 EMBL· GenBank· DDBJ | ABI49477.1 EMBL· GenBank· DDBJ | mRNA | ||
AY087775 EMBL· GenBank· DDBJ | AAM65311.1 EMBL· GenBank· DDBJ | mRNA |