Q9FGC7 · ZEP_ARATH

Function

function

Zeaxanthin epoxidase that plays an important role in the xanthophyll cycle and abscisic acid (ABA) biosynthesis. Converts zeaxanthin into antheraxanthin and subsequently violaxanthin. Required for resistance to osmotic and drought stresses, ABA-dependent stomatal closure, seed development and dormancy, modulation of defense gene expression and disease resistance and non-photochemical quencing (NPQ). Through its role in ABA biosynthesis, regulates the expression of stress-responsive genes such as RD29A during osmotic stress and is required for normal plant growth during vegetative development. Is required for late skotomorphogenic growth through its role in the xanthophyll carotenoids neoxanthin, violaxanthin and antheraxanthin biosynthesis. Required for beta-aminobutyric acid (BABA)-induced priming in disease resistance, tolerance to salt and drought stresses and sterility. Participates in NPQ by regulating the level of zeaxanthin in photosynthetic energy conversion. NPQ is a process that maintains the balance between dissipation and utilization of light energy to minimize the generation of oxidizing molecules and the molecular damages they can generate.

Miscellaneous

Plants overexpressing ZEP show increased levels of violaxanthin and ABA and increased tolerance to salt and drought stresses.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Pathway

Plant hormone biosynthesis; abscisate biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site82-110FAD (UniProtKB | ChEBI)
Binding site360-373FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentchloroplast envelope
Cellular Componentmembrane
Cellular Componentplastid
Molecular FunctionFAD binding
Molecular Functionzeaxanthin epoxidase activity
Biological Processabscisic acid biosynthetic process
Biological Processresponse to heat
Biological Processresponse to red light
Biological Processresponse to water deprivation
Biological Processsugar mediated signaling pathway
Biological Processxanthophyll biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Zeaxanthin epoxidase, chloroplastic
  • EC number
  • Short names
    AtZEP
  • Alternative names
    • Protein ABA DEFICIENT 1 (AtABA1)
    • Protein IMPAIRED IN BABA-INDUCED STERILITY 3
    • Protein LOW EXPRESSION OF OSMOTIC STRESS-RESPONSIVE GENES 6
    • Protein NON-PHOTOCHEMICAL QUENCHING 2

Gene names

    • Name
      ZEP
    • Synonyms
      ABA1, IBS3, LOS6, NPQ2
    • ORF names
      K8A10.10
    • Ordered locus names
      At5g67030

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Landsberg erecta
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9FGC7
  • Secondary accessions
    • F4K278
    • Q8RXE6
    • Q9FDX0
    • Q9FS21
    • Q9LDB9

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Increased endogenous level of zeaxanthin and reduced level of ABA. Reduced size of leaves, inflorescences and flowers, early flowering, increased number of wilted plants, premature seed germination and reduced osmotic water permeability and ability to close stomata. Reduced susceptibility to virulent isolates of P.parasitica and sensitivity to BABA-induced priming.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis125In aba1-3; ABA-deficient phenotype.
Mutagenesis160In aba1-6; ABA-deficient phenotype.
Mutagenesis386In los6; reduces plant size, accelerates flowering and increases transpirational water loss.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 25 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-59Chloroplast
ChainPRO_000041207260-667Zeaxanthin epoxidase, chloroplastic

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in leaves, stems and flowers, and at lower levels in roots and siliques.

Induction

By ABA and drought, salt and osmotic stresses.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain558-612FHA

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 2 isoforms produced by Alternative splicing.

Q9FGC7-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    667
  • Mass (Da)
    73,842
  • Last updated
    2001-03-01 v1
  • Checksum
    89A0A6E6B8A26D5D
MGSTPFCYSINPSPSKLDFTRTHVFSPVSKQFYLDLSSFSGKPGGVSGFRSRRALLGVKAATALVEKEEKREAVTEKKKKSRVLVAGGGIGGLVFALAAKKKGFDVLVFEKDLSAIRGEGKYRGPIQIQSNALAALEAIDIEVAEQVMEAGCITGDRINGLVDGISGTWYVKFDTFTPAASRGLPVTRVISRMTLQQILARAVGEDVIRNESNVVDFEDSGDKVTVVLENGQRYEGDLLVGADGIWSKVRNNLFGRSEATYSGYTCYTGIADFIPADIESVGYRVFLGHKQYFVSSDVGGGKMQWYAFHEEPAGGADAPNGMKKRLFEIFDGWCDNVLDLLHATEEEAILRRDIYDRSPGFTWGKGRVTLLGDSIHAMQPNMGQGGCMAIEDSFQLALELDEAWKQSVETTTPVDVVSSLKRYEESRRLRVAIIHAMARMAAIMASTYKAYLGVGLGPLSFLTKFRVPHPGRVGGRFFVDIAMPSMLDWVLGGNSEKLQGRPPSCRLTDKADDRLREWFEDDDALERTIKGEWYLIPHGDDCCVSETLCLTKDEDQPCIVGSEPDQDFPGMRIVIPSSQVSKMHARVIYKDGAFFLMDLRSEHGTYVTDNEGRRYRATPNFPARFRSSDIIEFGSDKKAAFRVKVIRKTPKSTRKNESNNDKLLQTA

Q9FGC7-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 581-610: SKMHARVIYKDGAFFLMDLRSEHGTYVTDN → YKLYACSCDLQRRSFLLDGSSKRTRNLCDR
    • 611-667: Missing

Sequence caution

The sequence AAL91193.1 differs from that shown. Reason: Miscellaneous discrepancy Intron retention.
The sequence AAO00920.1 differs from that shown. Reason: Miscellaneous discrepancy Intron retention.

Features

Showing features for sequence conflict, alternative sequence.

TypeIDPosition(s)Description
Sequence conflict29in Ref. 3; AAG17703/AAG38877
Sequence conflict42-54in Ref. 3; AAG17703/AAG38877
Sequence conflict76in Ref. 2; AAF82390/AAF82391
Sequence conflict77in Ref. 3; AAG17703/AAG38877
Sequence conflict116in Ref. 2; AAF82390/AAF82391
Sequence conflict180-182in Ref. 2; AAF82390/AAF82391
Sequence conflict194in Ref. 1; BAB11935
Alternative sequenceVSP_041638581-610in isoform 2
Alternative sequenceVSP_041639611-667in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB030296
EMBL· GenBank· DDBJ
BAB11935.1
EMBL· GenBank· DDBJ
mRNA
AF134577
EMBL· GenBank· DDBJ
AAF82390.1
EMBL· GenBank· DDBJ
Genomic DNA
AF134578
EMBL· GenBank· DDBJ
AAF82391.1
EMBL· GenBank· DDBJ
mRNA
AF281655
EMBL· GenBank· DDBJ
AAG17703.1
EMBL· GenBank· DDBJ
mRNA
AF283761
EMBL· GenBank· DDBJ
AAG38877.1
EMBL· GenBank· DDBJ
Genomic DNA
AB026640
EMBL· GenBank· DDBJ
BAB08942.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED98292.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED98293.1
EMBL· GenBank· DDBJ
Genomic DNA
AY081304
EMBL· GenBank· DDBJ
AAL91193.1
EMBL· GenBank· DDBJ
mRNA Sequence problems.
AY093145
EMBL· GenBank· DDBJ
AAM13144.1
EMBL· GenBank· DDBJ
mRNA
BT002560
EMBL· GenBank· DDBJ
AAO00920.1
EMBL· GenBank· DDBJ
mRNA Sequence problems.

Genome annotation databases

Similar Proteins

Disclaimer

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