Q9FFK8 · NFXL2_ARATH

Function

function

Probable transcriptional regulator. May mediate E2- or E3-dependent ubiquitination. Required to gate light sensitivity during the night. Regulates the speed of the clock by acting in the feedback loop between CCA1, LHY and APRR1/TOC1. Promotes the expression of CCA1 at night but not by days. This activational effect is enhanced by interaction with ADO1/ZTL. Association with ADO1/ZTL is not leading to the degradation of NFXL2. Confers sensitivity to osmotic stress such as high salinity. Prevents H2O2 production and abscisic acid accumulation. Part of a regulatory network that integrates the biosynthesis and action of abscisic acid, reactive oxygen species and cuticle components.

Pathway

Protein modification; protein ubiquitination.

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentmembrane
Cellular Componentnucleus
Molecular FunctionDNA-binding transcription factor activity, RNA polymerase II-specific
Molecular FunctionRNA polymerase II transcription regulatory region sequence-specific DNA binding
Molecular Functiontransferase activity
Molecular Functionzinc ion binding
Biological Processprotein ubiquitination
Biological Processregulation of DNA-templated transcription
Biological Processregulation of hydrogen peroxide metabolic process
Biological Processresponse to salt stress
Biological Processrhythmic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NF-X1-type zinc finger protein NFXL2
  • EC number
  • Short names
    AtNFXL2
  • Alternative names
    • Protein EARLY BIRD

Gene names

    • Name
      NFXL2
    • Synonyms
      EBI
    • ORF names
      MJJ3.6
    • Ordered locus names
      At5g05660

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9FFK8
  • Secondary accessions
    • F4K0S4

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Nucleus
Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane841-863Helical

Keywords

Phenotypes & Variants

Disruption phenotype

No obvious morphological alterations. Enhanced growth and survival under water or salt stress. Enhanced H2O2 production. Elevated abscisic acid levels and reduced stomatal aperture.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis354In ebi-1; altered circadian clock function.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 63 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003968361-883NF-X1-type zinc finger protein NFXL2

Proteomic databases

Expression

Tissue specificity

Constitutively expressed in mesophyll and guard cells.

Induction

Circadian-regulation with a peak of expression at or before dawn. Not regulated by biotic and abiotic stresses, by light and nutrient conditions or upon treatment with elicitors, chemicals, abscisic acid or phytohormones.

Gene expression databases

Interaction

Subunit

Interacts with ADO1/ZTL.

Protein-protein interaction databases

Family & Domains

Features

Showing features for compositional bias, region, zinc finger.

TypeIDPosition(s)Description
Compositional bias1-18Polar residues
Region1-44Disordered
Zinc finger87-152RING-type; degenerate
Zinc finger198-216NF-X1-type 1
Zinc finger250-269NF-X1-type 2
Zinc finger303-322NF-X1-type 3
Zinc finger357-377NF-X1-type 4
Zinc finger410-429NF-X1-type 5
Zinc finger437-456NF-X1-type 6
Zinc finger494-515NF-X1-type 7
Zinc finger523-568NF-X1-type 8
Zinc finger605-636NF-X1-type 9
Zinc finger646-664NF-X1-type 10
Zinc finger709-738NF-X1-type 11
Region798-824Disordered

Domain

The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.

Sequence similarities

Belongs to the NFX1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing. Additional isoforms seem to exist.

Q9FFK8-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    NFXL2-97
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    883
  • Mass (Da)
    99,229
  • Last updated
    2010-08-10 v2
  • Checksum
    65CA28E09D161CB7
MTNMAGTATTEFRWKSPPQPPSQEQPISDSDSDSGSDSENHQHRHNDLSNSIFEAYLDCHSSSSPSSIDLAKIQSFLASSSSGAVSCLICLERIKRTDPTWSCTSSCFAVFHLFCIQSWARQCLDLQAARAVTRPSSNPTEPEAVWNCPKCRSSYQKSKIPRRYLCYCGKEEDPPADNPWILPHSCGEVCERPLSNNCGHCCLLLCHPGPCASCPKLVKAKCFCGGVEDVRRCGHKQFSCGDVCERVLDCNIHNCREICHDGECPPCRERAVYKCSCGKVKEEKDCCERVFRCEASCENMLNCGKHVCERGCHAGECGLCPYQGKRSCPCGKRFYQGLSCDVVAPLCGGTCDKVLGCGYHRCPERCHRGPCLETCRIVVTKSCRCGVTKKQVPCHQELACERKCQRVRDCARHACRRRCCDGECPPCSEICGKKLRCRNHKCQSPCHQGPCAPCPIMVTISCACGETHFEVPCGTETNQKPPRCRKLCHITPLCRHGQNQKPHKCHYGACPPCRLLCDEEYPCGHKCKLRCHGPRPPPNREFILKPTKKMLHIQAESTPGSPCPRCPEPVWRPCVGHHLAAEKRMICSDRTQFACDNLCGNPLPCGNHYCSYFCHALDIRSSSLDKRSESCEKCDLRCQKERTPRCQHPCPRRCHPEDCPPCKTLVKRSCHCGAMVHAFECIYYNTMSEKDQMKARSCRGPCHRKLPNCTHLCPEICHPGQCPLPEKCGKKVVVRCKCLTLKKEWVCQDVQAAHRATGSDPKEVPKNQFGVGLLPCDSNCKSKLQVAESVLTQRNVKEIEEKEEPSGKNASKRRKRRGRGQDIQETTRLQKLAVTTKRILMVVMLVAMLAAVSYYGYKGLLWLSDWMNEVEEQRQKSRRYPRI

Q9FFK8-2

  • Name
    NFXL2-100
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 730-730: K → KKVSICQFISIWDAVSVTSEILLVSILSWI

Q9FFK8-3

  • Name
    NFXL2-78
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 698-714: CRGPCHRKLPNCTHLCP → SLVIPTVRANYRWLNRC
    • 715-883: Missing

Sequence caution

The sequence AK228690 differs from that shown. Reason: Frameshift
The sequence BAB09660.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for compositional bias, sequence conflict, alternative sequence.

TypeIDPosition(s)Description
Compositional bias1-18Polar residues
Sequence conflict270in Ref. 1; no nucleotide entry
Sequence conflict466in Ref. 4; AK228690
Sequence conflict552in Ref. 1; no nucleotide entry
Sequence conflict660in Ref. 1; no nucleotide entry
Alternative sequenceVSP_046010698-714in isoform NFXL2-78
Alternative sequenceVSP_046011715-883in isoform NFXL2-78
Alternative sequenceVSP_046012730in isoform NFXL2-100

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB005237
EMBL· GenBank· DDBJ
BAB09660.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002688
EMBL· GenBank· DDBJ
AED90905.2
EMBL· GenBank· DDBJ
Genomic DNA
AK228690
EMBL· GenBank· DDBJ
-mRNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp