Q9FFK8 · NFXL2_ARATH
- ProteinNF-X1-type zinc finger protein NFXL2
- GeneNFXL2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids883 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Probable transcriptional regulator. May mediate E2- or E3-dependent ubiquitination. Required to gate light sensitivity during the night. Regulates the speed of the clock by acting in the feedback loop between CCA1, LHY and APRR1/TOC1. Promotes the expression of CCA1 at night but not by days. This activational effect is enhanced by interaction with ADO1/ZTL. Association with ADO1/ZTL is not leading to the degradation of NFXL2. Confers sensitivity to osmotic stress such as high salinity. Prevents H2O2 production and abscisic acid accumulation. Part of a regulatory network that integrates the biosynthesis and action of abscisic acid, reactive oxygen species and cuticle components.
Pathway
Protein modification; protein ubiquitination.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Cellular Component | nucleus | |
Molecular Function | DNA-binding transcription factor activity, RNA polymerase II-specific | |
Molecular Function | RNA polymerase II transcription regulatory region sequence-specific DNA binding | |
Molecular Function | transferase activity | |
Molecular Function | zinc ion binding | |
Biological Process | protein ubiquitination | |
Biological Process | regulation of DNA-templated transcription | |
Biological Process | regulation of hydrogen peroxide metabolic process | |
Biological Process | response to salt stress | |
Biological Process | rhythmic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNF-X1-type zinc finger protein NFXL2
- EC number
- Short namesAtNFXL2
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9FFK8
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Phenotypes & Variants
Disruption phenotype
No obvious morphological alterations. Enhanced growth and survival under water or salt stress. Enhanced H2O2 production. Elevated abscisic acid levels and reduced stomatal aperture.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 354 | In ebi-1; altered circadian clock function. | ||||
Sequence: V → I |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 63 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000396836 | 1-883 | NF-X1-type zinc finger protein NFXL2 | |||
Sequence: MTNMAGTATTEFRWKSPPQPPSQEQPISDSDSDSGSDSENHQHRHNDLSNSIFEAYLDCHSSSSPSSIDLAKIQSFLASSSSGAVSCLICLERIKRTDPTWSCTSSCFAVFHLFCIQSWARQCLDLQAARAVTRPSSNPTEPEAVWNCPKCRSSYQKSKIPRRYLCYCGKEEDPPADNPWILPHSCGEVCERPLSNNCGHCCLLLCHPGPCASCPKLVKAKCFCGGVEDVRRCGHKQFSCGDVCERVLDCNIHNCREICHDGECPPCRERAVYKCSCGKVKEEKDCCERVFRCEASCENMLNCGKHVCERGCHAGECGLCPYQGKRSCPCGKRFYQGLSCDVVAPLCGGTCDKVLGCGYHRCPERCHRGPCLETCRIVVTKSCRCGVTKKQVPCHQELACERKCQRVRDCARHACRRRCCDGECPPCSEICGKKLRCRNHKCQSPCHQGPCAPCPIMVTISCACGETHFEVPCGTETNQKPPRCRKLCHITPLCRHGQNQKPHKCHYGACPPCRLLCDEEYPCGHKCKLRCHGPRPPPNREFILKPTKKMLHIQAESTPGSPCPRCPEPVWRPCVGHHLAAEKRMICSDRTQFACDNLCGNPLPCGNHYCSYFCHALDIRSSSLDKRSESCEKCDLRCQKERTPRCQHPCPRRCHPEDCPPCKTLVKRSCHCGAMVHAFECIYYNTMSEKDQMKARSCRGPCHRKLPNCTHLCPEICHPGQCPLPEKCGKKVVVRCKCLTLKKEWVCQDVQAAHRATGSDPKEVPKNQFGVGLLPCDSNCKSKLQVAESVLTQRNVKEIEEKEEPSGKNASKRRKRRGRGQDIQETTRLQKLAVTTKRILMVVMLVAMLAAVSYYGYKGLLWLSDWMNEVEEQRQKSRRYPRI |
Proteomic databases
Expression
Tissue specificity
Constitutively expressed in mesophyll and guard cells.
Induction
Circadian-regulation with a peak of expression at or before dawn. Not regulated by biotic and abiotic stresses, by light and nutrient conditions or upon treatment with elicitors, chemicals, abscisic acid or phytohormones.
Gene expression databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-18 | Polar residues | ||||
Sequence: MTNMAGTATTEFRWKSPP | ||||||
Region | 1-44 | Disordered | ||||
Sequence: MTNMAGTATTEFRWKSPPQPPSQEQPISDSDSDSGSDSENHQHR | ||||||
Zinc finger | 87-152 | RING-type; degenerate | ||||
Sequence: CLICLERIKRTDPTWSCTSSCFAVFHLFCIQSWARQCLDLQAARAVTRPSSNPTEPEAVWNCPKCR | ||||||
Zinc finger | 198-216 | NF-X1-type 1 | ||||
Sequence: CGHCCLLLCHPGPCASCPK | ||||||
Zinc finger | 250-269 | NF-X1-type 2 | ||||
Sequence: CNIHNCREICHDGECPPCRE | ||||||
Zinc finger | 303-322 | NF-X1-type 3 | ||||
Sequence: CGKHVCERGCHAGECGLCPY | ||||||
Zinc finger | 357-377 | NF-X1-type 4 | ||||
Sequence: CGYHRCPERCHRGPCLETCRI | ||||||
Zinc finger | 410-429 | NF-X1-type 5 | ||||
Sequence: CARHACRRRCCDGECPPCSE | ||||||
Zinc finger | 437-456 | NF-X1-type 6 | ||||
Sequence: CRNHKCQSPCHQGPCAPCPI | ||||||
Zinc finger | 494-515 | NF-X1-type 7 | ||||
Sequence: CRHGQNQKPHKCHYGACPPCRL | ||||||
Zinc finger | 523-568 | NF-X1-type 8 | ||||
Sequence: CGHKCKLRCHGPRPPPNREFILKPTKKMLHIQAESTPGSPCPRCPE | ||||||
Zinc finger | 605-636 | NF-X1-type 9 | ||||
Sequence: CGNHYCSYFCHALDIRSSSLDKRSESCEKCDL | ||||||
Zinc finger | 646-664 | NF-X1-type 10 | ||||
Sequence: CQHPCPRRCHPEDCPPCKT | ||||||
Zinc finger | 709-738 | NF-X1-type 11 | ||||
Sequence: CTHLCPEICHPGQCPLPEKCGKKVVVRCKC | ||||||
Region | 798-824 | Disordered | ||||
Sequence: EIEEKEEPSGKNASKRRKRRGRGQDIQ |
Domain
The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
Sequence similarities
Belongs to the NFX1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing. Additional isoforms seem to exist.
Q9FFK8-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameNFXL2-97
- Length883
- Mass (Da)99,229
- Last updated2010-08-10 v2
- Checksum65CA28E09D161CB7
Q9FFK8-2
- NameNFXL2-100
- Differences from canonical
- 730-730: K → KKVSICQFISIWDAVSVTSEILLVSILSWI
Q9FFK8-3
- NameNFXL2-78
Sequence caution
Features
Showing features for compositional bias, sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-18 | Polar residues | ||||
Sequence: MTNMAGTATTEFRWKSPP | ||||||
Sequence conflict | 270 | in Ref. 1; no nucleotide entry | ||||
Sequence: R → H | ||||||
Sequence conflict | 466 | in Ref. 4; AK228690 | ||||
Sequence: E → G | ||||||
Sequence conflict | 552 | in Ref. 1; no nucleotide entry | ||||
Sequence: H → N | ||||||
Sequence conflict | 660 | in Ref. 1; no nucleotide entry | ||||
Sequence: P → L | ||||||
Alternative sequence | VSP_046010 | 698-714 | in isoform NFXL2-78 | |||
Sequence: CRGPCHRKLPNCTHLCP → SLVIPTVRANYRWLNRC | ||||||
Alternative sequence | VSP_046011 | 715-883 | in isoform NFXL2-78 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_046012 | 730 | in isoform NFXL2-100 | |||
Sequence: K → KKVSICQFISIWDAVSVTSEILLVSILSWI |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB005237 EMBL· GenBank· DDBJ | BAB09660.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002688 EMBL· GenBank· DDBJ | AED90905.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK228690 EMBL· GenBank· DDBJ | - | mRNA | No translation available. |