Q9F6D4 · FABH_STRLI

  • Protein
    Beta-ketoacyl-[acyl-carrier-protein] synthase III
  • Gene
    fabH
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities (PubMed:11732905).
Propionyl-CoA and isobutyryl-CoA were the two most preferred substrates, although acetyl-CoA and butyryl-CoA could also be accepted and elongated (PubMed:11732905).
Involved in the biosynthesis of R1128 polyketide (PubMed:11732905).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
26 μMpropanoyl-CoA
184 μMbutanoyl-CoA
230 μMacetyl-CoA
kcat is 74 min-1 with propanoyl-CoA as substrate. kcat is 113 min-1 with butanoyl-CoA as substrate. kcat is 58 min-1 with acetyl-CoA as substrate.

Pathway

Lipid metabolism; fatty acid biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site121
Active site257
Active site288

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function3-oxoacyl-[acyl-carrier-protein] synthase activity
Molecular Functionbeta-ketoacyl-acyl-carrier-protein synthase III activity
Biological Processfatty acid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Beta-ketoacyl-[acyl-carrier-protein] synthase III
  • EC number
  • Short names
    Beta-ketoacyl-ACP synthase III
    ; KAS III
  • Alternative names
    • 3-oxoacyl-[acyl-carrier-protein] synthase 3
    • 3-oxoacyl-[acyl-carrier-protein] synthase III
    • Branched-chain beta-ketoacyl-[acyl-carrier-protein] synthase

Gene names

    • Name
      fabH
    • Synonyms
      zhuH

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    Q9F6D4

Subcellular Location

Keywords

Phenotypes & Variants

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001104891-339Beta-ketoacyl-[acyl-carrier-protein] synthase III

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region258-262ACP-binding

Domain

The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH.

Sequence similarities

Belongs to the thiolase-like superfamily. FabH family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    339
  • Mass (Da)
    35,392
  • Last updated
    2001-03-01 v1
  • Checksum
    F0CA49FD80A7A26D
MPGLRVPERRFSRVLGVGSYRPRREVSNKEVCTWIDSTEEWIETRTGIRSRRIAEPDETIQVMGVAASRRALEHAGVDPAEIDLVVVSTMTNFVHTPPLSVAIAHELGADNAGGFDLSAACAGFCHALSIAADAVESGGSRHVLVVATERMTDVIDLADRSLSFLFGDGAGAAVVGPSDVPGIGPVVRGIDGTGLGSLHMSSSWDQYVEDPSVGRPALVMDGKRVFRWAVADVVPAAREALEVAGLTVGDLVAFVPHQANLRIIDVLVDRLGVPEHVVVSRDAEDTGNTSSASVALALDRLVRSGAVPGGGPALMIGFGAGLSYAGQALLLPDPPSTPA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF293442
EMBL· GenBank· DDBJ
AAG30195.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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