Q9ERH6 · MOAP1_MOUSE
- ProteinModulator of apoptosis 1
- GeneMoap1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids352 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Retrotransposon-derived protein that forms virion-like capsids (PubMed:34413232).
Acts as an effector of BAX during apoptosis: enriched at outer mitochondria membrane and associates with BAX upon induction of apoptosis, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (By similarity).
Required for death receptor-dependent apoptosis (By similarity).
When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation (By similarity).
Also promotes autophagy: promotes phagophore closure via association with ATG8 proteins (PubMed:33783314).
Acts as an inhibitor of the NFE2L2/NRF2 pathway via interaction with SQSTM1: interaction promotes dissociation of SQSTM1 inclusion bodies that sequester KEAP1, relieving inactivation of the BCR(KEAP1) complex (By similarity).
Acts as an effector of BAX during apoptosis: enriched at outer mitochondria membrane and associates with BAX upon induction of apoptosis, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (By similarity).
Required for death receptor-dependent apoptosis (By similarity).
When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation (By similarity).
Also promotes autophagy: promotes phagophore closure via association with ATG8 proteins (PubMed:33783314).
Acts as an inhibitor of the NFE2L2/NRF2 pathway via interaction with SQSTM1: interaction promotes dissociation of SQSTM1 inclusion bodies that sequester KEAP1, relieving inactivation of the BCR(KEAP1) complex (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrial outer membrane | |
Cellular Component | nucleus | |
Molecular Function | ubiquitin protein ligase binding | |
Biological Process | apoptotic process | |
Biological Process | autophagy | |
Biological Process | extrinsic apoptotic signaling pathway in absence of ligand | |
Biological Process | extrinsic apoptotic signaling pathway via death domain receptors | |
Biological Process | intrinsic apoptotic signaling pathway in response to DNA damage | |
Biological Process | positive regulation of apoptotic process | |
Biological Process | positive regulation of autophagosome assembly | |
Biological Process | positive regulation of release of cytochrome c from mitochondria | |
Biological Process | protein insertion into mitochondrial membrane | |
Biological Process | regulation of apoptotic process |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameModulator of apoptosis 1
- Short namesMAP-1 ; MmMOAP1
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9ERH6
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Forms virion-like extracellular vesicles that are released from cells.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000155207 | 1-352 | Modulator of apoptosis 1 | |||
Sequence: MTLRLLEDWCRGMDMNPRKALLVAGIPPTCGVADIEEALQAGLAPLGEHRLLGRMFRRDENKNVALIGLTVETGSALVPKEIPAKGGVWRVIFKPPDTDSDFLCRLNEFLKGEGMTMGELTRVLGNRNDPLGLDPGIMIPEIRAPMLAQALNEALKPTLQYLRYKKLSVFSGRDPPGPGEEEFESWMFHTSQVMKTWQVSDVEKRRRLIESLRGPAFEIIRVLKINNPFITVAECLKTLETIFGIIDNPRALQVKYLTTYQKTDEKLSAYVLRLEPLLQKLVQKGAIEKEVVNQARLDQVIAGAVHKSVRRELGLPEGSPAPGLLQLLTLIKDKEAEEEEVLLQAELEGYCT |
Post-translational modification
Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1, this modification is inhibited by TRIM39.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Widely expressed, including in the brain. High expression levels in testis.
Gene expression databases
Interaction
Subunit
Homodimer. Under normal circumstances, held in an inactive conformation by an intramolecular interaction. Interacts with BAX. Binding to RASSF1 isoform A (RASSF1A) relieves this inhibitory interaction and allows further binding to BAX. Binds also to BCL2 and BCLX. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Interacts with TRIM39 (By similarity).
Interacts with RASSF6 (PubMed:17404571).
Interacts with ATG8 proteins MAP1LC3A, MAP1LC3B and MAP1LC3C (By similarity).
Does not interact with ATG8 proteins GABARAPL1, GABARAPL2 and GABARAP (By similarity).
Interacts with SQSTM1; promoting dissociation of SQSTM1 inclusion bodies that sequester KEAP1 (By similarity).
Interacts with RASSF6 (PubMed:17404571).
Interacts with ATG8 proteins MAP1LC3A, MAP1LC3B and MAP1LC3C (By similarity).
Does not interact with ATG8 proteins GABARAPL1, GABARAPL2 and GABARAP (By similarity).
Interacts with SQSTM1; promoting dissociation of SQSTM1 inclusion bodies that sequester KEAP1 (By similarity).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for motif, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 49-52 | LIR | ||||
Sequence: HRLL | ||||||
Region | 120-127 | BH3-like | ||||
Sequence: LTRVLGNR | ||||||
Region | 204-207 | RASSF1-binding | ||||
Sequence: KRRR |
Domain
The protein is evolutionarily related to retrotransposon Gag proteins: it contains the capsid (CA)subdomain of gag.
The BH3-like domain is required for association with BAX and for mediating apoptosis. The three BH domains (BH1, BH2, and BH3) of BAX are all required for mediating protein-protein interaction.
The LIR motif (LC3-interacting region) is required for the interaction with the ATG8 family proteins MAP1LC3A, MAP1LC3B and MAP1LC3C.
Sequence similarities
Belongs to the PNMA family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length352
- Mass (Da)39,404
- Last updated2001-03-01 v1
- Checksum8F4630D080495D98
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 57 | in Ref. 3; AAH55374 | ||||
Sequence: R → K |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF305551 EMBL· GenBank· DDBJ | AAG31787.1 EMBL· GenBank· DDBJ | mRNA | ||
AK019599 EMBL· GenBank· DDBJ | BAB31810.1 EMBL· GenBank· DDBJ | mRNA | ||
BC014715 EMBL· GenBank· DDBJ | AAH14715.1 EMBL· GenBank· DDBJ | mRNA | ||
BC055374 EMBL· GenBank· DDBJ | AAH55374.1 EMBL· GenBank· DDBJ | mRNA |