Q9EQN3 · T22D4_MOUSE
- ProteinTSC22 domain family protein 4
- GeneTsc22d4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids387 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Binds DNA and acts as a transcriptional repressor (PubMed:27827363).
Involved in the regulation of systematic glucose homeostasis and insulin sensitivity, via transcriptional repression of downstream insulin signaling targets such as OBP2A/LCN13 (PubMed:27827363).
Acts as a negative regulator of lipogenic gene expression in hepatocytes and thereby mediates the control of very low-density lipoprotein release (PubMed:23307490).
May play a role in neurite elongation and survival (PubMed:20878296).
Involved in the regulation of systematic glucose homeostasis and insulin sensitivity, via transcriptional repression of downstream insulin signaling targets such as OBP2A/LCN13 (PubMed:27827363).
Acts as a negative regulator of lipogenic gene expression in hepatocytes and thereby mediates the control of very low-density lipoprotein release (PubMed:23307490).
May play a role in neurite elongation and survival (PubMed:20878296).
Miscellaneous
Involved in the development of hyperglycaemia and insulin resistance in diabetic mouse models (PubMed:27827363).
May be involved in altered hepatic lipid handling as part of cancer-induced cachexia (PubMed:23307490).
May be involved in altered hepatic lipid handling as part of cancer-induced cachexia (PubMed:23307490).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | dendrite | |
Cellular Component | nucleus | |
Cellular Component | synapse | |
Biological Process | glucose homeostasis | |
Biological Process | negative regulation of transcription by RNA polymerase II | |
Biological Process | neuron cellular homeostasis | |
Biological Process | neuron projection extension |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameTSC22 domain family protein 4
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9EQN3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes away from the nucleus to neurite processes and synaptic termini as cerebellar granular neurons differentiate (PubMed:20878296).
Accumulates in the cytoplasm of differentiated Purkinje cells (PubMed:20878296).
Localized to both the cytoplasm and nucleus in immature cerebellar granular neurons and atrophic Purkinje cells (PubMed:20878296).
Accumulates in the cytoplasm of differentiated Purkinje cells (PubMed:20878296).
Localized to both the cytoplasm and nucleus in immature cerebellar granular neurons and atrophic Purkinje cells (PubMed:20878296).
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000219375 | 1-387 | TSC22 domain family protein 4 | |||
Sequence: MSGGKKKSSFQITSVTTDYEGPGSPGASDSPVPPALAGPPPRLPNGDPNPDPGGRGTPRNGSPPPGAPASRFRVVKLPQGLGEPYRRGRWTCVDVYERDLEPPSFGRLLEGIRGASGGTGGRSLDSRLELASLGISTPIPQPGLSQGPTSWLRPPPTSPGPQARSFTGGLGQLAGPGKAKVETPPLSASPPQQRPPGPGTGDSAQTLPSLRVEVESGGSAAATPPLSRRRDGAVRLRMELVAPAETGKVPPTDSRPNSPALYFDASLVHKSPDPFGAAAAQSLSLARSMLAISGHLDSDDDSGSGSLVGIDNKIEQAMDLVKSHLMFAVREEVEVLKEQIRDLAERNAALEQENGLLRALASPEQLAQLPSSGLPRLGPSAPNGPSI | ||||||
Modified residue | 57 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 62 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 165 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 183 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 187 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 189 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 219 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 223 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 254 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 258 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 271 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 362 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the liver (at protein level) (PubMed:23307490, PubMed:27827363).
Expressed in Purkinje cells and proliferating cerebellar granular neurons (at protein level) (PubMed:20878296).
Expressed in the cortex, medulla and papilla of the kidney
Expressed in Purkinje cells and proliferating cerebellar granular neurons (at protein level) (PubMed:20878296).
Expressed in the cortex, medulla and papilla of the kidney
Induction
Induced by TGF-beta treatment (PubMed:11707329).
Induced by a wasting-associated liver metabolism as a result of a methionine-choline deficient diet or cancer-induced cachectic phenotype (PubMed:23307490).
Induced by renal hyperosmotic stress (PubMed:17147695).
Induced by a wasting-associated liver metabolism as a result of a methionine-choline deficient diet or cancer-induced cachectic phenotype (PubMed:23307490).
Induced by renal hyperosmotic stress (PubMed:17147695).
Developmental stage
Expression starts at 8.5 dpc and undergoes a second peak of activation at 12.5 dpc (PubMed:11707329).
At 12.5 dpc, expression encompasses the entire central nervous system, with highest levels in the dorsal root and trigeminal ganglia (PubMed:11707329).
Expressed in the granule neurons and Purkinje cells in the external and internal granular layers of the cerebellum from postnatal day 6 (PubMed:20878296).
At 12.5 dpc, expression encompasses the entire central nervous system, with highest levels in the dorsal root and trigeminal ganglia (PubMed:11707329).
Expressed in the granule neurons and Purkinje cells in the external and internal granular layers of the cerebellum from postnatal day 6 (PubMed:20878296).
Gene expression databases
Interaction
Subunit
Forms a homodimer or heterodimer (By similarity).
Forms a heterodimer with TSC22D1 isoforms 1 and 2 (By similarity).
Interacts with NRBP1 (By similarity).
Forms a heterodimer with TSC22D1 isoforms 1 and 2 (By similarity).
Interacts with NRBP1 (By similarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9EQN3 | Aifm1 Q9Z0X1 | 4 | EBI-7821198, EBI-773597 | |
BINARY | Q9EQN3 | Tsc22d1 P62500 | 2 | EBI-7821198, EBI-8296837 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-23 | Polar residues | ||||
Sequence: MSGGKKKSSFQITSVTTDYEGPG | ||||||
Region | 1-85 | Disordered | ||||
Sequence: MSGGKKKSSFQITSVTTDYEGPGSPGASDSPVPPALAGPPPRLPNGDPNPDPGGRGTPRNGSPPPGAPASRFRVVKLPQGLGEPY | ||||||
Compositional bias | 27-48 | Pro residues | ||||
Sequence: ASDSPVPPALAGPPPRLPNGDP | ||||||
Region | 135-232 | Disordered | ||||
Sequence: ISTPIPQPGLSQGPTSWLRPPPTSPGPQARSFTGGLGQLAGPGKAKVETPPLSASPPQQRPPGPGTGDSAQTLPSLRVEVESGGSAAATPPLSRRRDG | ||||||
Compositional bias | 198-212 | Polar residues | ||||
Sequence: PGTGDSAQTLPSLRV | ||||||
Region | 336-357 | Leucine-zipper | ||||
Sequence: LKEQIRDLAERNAALEQENGLL | ||||||
Region | 368-387 | Disordered | ||||
Sequence: QLPSSGLPRLGPSAPNGPSI |
Sequence similarities
Belongs to the TSC-22/Dip/Bun family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length387
- Mass (Da)39,979
- Last updated2011-07-27 v2
- ChecksumD4160FA3AB2DFB90
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-23 | Polar residues | ||||
Sequence: MSGGKKKSSFQITSVTTDYEGPG | ||||||
Compositional bias | 27-48 | Pro residues | ||||
Sequence: ASDSPVPPALAGPPPRLPNGDP | ||||||
Sequence conflict | 97 | in Ref. 1; AAK02018 | ||||
Sequence: E → K | ||||||
Compositional bias | 198-212 | Polar residues | ||||
Sequence: PGTGDSAQTLPSLRV | ||||||
Sequence conflict | 339 | in Ref. 2; AAG41219 | ||||
Sequence: Q → H |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF315352 EMBL· GenBank· DDBJ | AAK02018.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AF201286 EMBL· GenBank· DDBJ | AAG41219.1 EMBL· GenBank· DDBJ | mRNA | ||
BC018544 EMBL· GenBank· DDBJ | AAH18544.1 EMBL· GenBank· DDBJ | mRNA | ||
BC023761 EMBL· GenBank· DDBJ | AAH23761.1 EMBL· GenBank· DDBJ | mRNA | ||
BC145721 EMBL· GenBank· DDBJ | AAI45722.1 EMBL· GenBank· DDBJ | mRNA | ||
BC145723 EMBL· GenBank· DDBJ | AAI45724.1 EMBL· GenBank· DDBJ | mRNA | ||
AK018735 EMBL· GenBank· DDBJ | BAB31377.1 EMBL· GenBank· DDBJ | mRNA | ||
AK143608 EMBL· GenBank· DDBJ | BAE25460.1 EMBL· GenBank· DDBJ | mRNA | ||
CH466529 EMBL· GenBank· DDBJ | EDL19240.1 EMBL· GenBank· DDBJ | Genomic DNA |