Q9EQC1 · 3BHS7_MOUSE
- Protein3 beta-hydroxysteroid dehydrogenase type 7
- GeneHsd3b7
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids369 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis (PubMed:11067870).
Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953).
Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953).
Catalytic activity
- 7alpha-hydroxycholesterol + NAD+ = 7alpha-hydroxycholest-4-en-3-one + H+ + NADHThis reaction proceeds in the forward direction.
- 7alpha,25-dihydroxycholesterol + NAD+ = 7alpha,25-dihydroxy-4-cholesten-3-one + H+ + NADHThis reaction proceeds in the forward direction.
- (25R)-cholest-5-en-3beta,7alpha,26-triol + NAD+ = (25R)-7alpha,26-dihydroxycholest-4-en-3-one + H+ + NADHThis reaction proceeds in the forward direction.
- (24S)-7alpha-dihydroxycholesterol + NAD+ = (24S)-7alpha,24-dihydroxycholest-4-en-3-one + H+ + NADHThis reaction proceeds in the forward direction.
Pathway
Lipid metabolism; steroid biosynthesis.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | lipid droplet | |
Molecular Function | cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase activity | |
Molecular Function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | |
Biological Process | B cell chemotaxis | |
Biological Process | cholesterol catabolic process | |
Biological Process | regulation of cell growth | |
Biological Process | steroid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended name3 beta-hydroxysteroid dehydrogenase type 7
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9EQC1
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 289-309 | Helical | ||||
Sequence: LLPYWLLVLLATLNALLQWLL | ||||||
Transmembrane | 312-334 | Helical | ||||
Sequence: LVLYTPLLNPYTLAMANTTFTVS |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000087792 | 1-369 | 3 beta-hydroxysteroid dehydrogenase type 7 | |||
Sequence: MADSAQVPTLVYLVTGGCGFLGEHIVRMLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQAHEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRLIGAHPLLPYWLLVLLATLNALLQWLLRPLVLYTPLLNPYTLAMANTTFTVSTNKAQRHFGYKPLFSWEESRTRTIQWVQAMEGSAR |
Proteomic databases
PTM databases
Expression
Structure
Sequence
- Sequence statusComplete
- Length369
- Mass (Da)41,135
- Last updated2001-03-01 v1
- Checksum5857A690E0600F13
Computationally mapped potential isoform sequences
There are 7 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0U1RQA2 | A0A0U1RQA2_MOUSE | Hsd3b7 | 141 | ||
A0A0U1RNL4 | A0A0U1RNL4_MOUSE | Hsd3b7 | 119 | ||
A0A0U1RP28 | A0A0U1RP28_MOUSE | Hsd3b7 | 70 | ||
D3YU71 | D3YU71_MOUSE | Hsd3b7 | 100 | ||
D3YVB8 | D3YVB8_MOUSE | Hsd3b7 | 77 | ||
D3Z3X1 | D3Z3X1_MOUSE | Hsd3b7 | 338 | ||
D3Z3X2 | D3Z3X2_MOUSE | Hsd3b7 | 221 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF277718 EMBL· GenBank· DDBJ | AAG37823.1 EMBL· GenBank· DDBJ | mRNA | ||
BC132605 EMBL· GenBank· DDBJ | AAI32606.1 EMBL· GenBank· DDBJ | mRNA | ||
BC138589 EMBL· GenBank· DDBJ | AAI38590.1 EMBL· GenBank· DDBJ | mRNA |