Q9EQ76 · FMO3_RAT

  • Protein
    Flavin-containing monooxygenase 3
  • Gene
    Fmo3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Essential hepatic enzyme that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including drugs as well as dietary compounds (PubMed:3739217, PubMed:7736906).
Plays an important role in the metabolism of trimethylamine (TMA), via the production of trimethylamine N-oxide (TMAO) metabolite. TMA is generated by the action of gut microbiota using dietary precursors such as choline, choline containing compounds, betaine or L-carnitine. By regulating TMAO concentration, FMO3 directly impacts both platelet responsiveness and rate of thrombus formation

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site9-13FAD (UniProtKB | ChEBI)
Binding site32FAD (UniProtKB | ChEBI)
Binding site40-41FAD (UniProtKB | ChEBI)
Binding site60-61NADP+ (UniProtKB | ChEBI)
Binding site61-62FAD (UniProtKB | ChEBI)
Binding site195-198NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Molecular Functionalbendazole monooxygenase activity
Molecular Functionamino acid binding
Molecular Functionflavin adenine dinucleotide binding
Molecular Functionhypotaurine dehydrogenase activity
Molecular Functionmonooxygenase activity
Molecular FunctionN,N-dimethylaniline monooxygenase activity
Molecular FunctionNADP binding
Molecular Functiontrimethylamine monooxygenase activity
Biological Processtaurine biosynthetic process
Biological Processxenobiotic metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Flavin-containing monooxygenase 3
  • EC number
  • Alternative names
    • Dimethylaniline monooxygenase [N-oxide-forming] 3
    • Dimethylaniline oxidase 3
    • Hepatic flavin-containing monooxygenase 3 (FMO 3)
    • Trimethylamine monooxygenase

Gene names

    • Name
      Fmo3

Organism names

  • Taxonomic identifier
  • Strain
    • Sprague-Dawley
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    Q9EQ76
  • Secondary accessions
    • Q5PQV6

Proteomes

Organism-specific databases

Subcellular Location

Microsome membrane
; Single-pass membrane protein
Endoplasmic reticulum membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane511-531Helical

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00001476591-531Flavin-containing monooxygenase 3
Modified residue401Phosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Detected in liver and kidney (at protein level) (PubMed:19307449).
Expressed in kidney and liver. Weakly expressed in lung. Does not seem to be expressed in brain, adipose tissue, or muscle

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the FMO family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    531
  • Mass (Da)
    59,960
  • Last updated
    2001-03-01 v1
  • Checksum
    35A89988323B311F
MKRKVAVIGAGVSGLAAIRSCLEEGLEPTCFERSDDVGGLWKFSDHTEEGRASIYQSVFTNSSKEMMCFPDFPYPDDFPNFMHNSKLQEYITSFATEKNLLKYIQFETLVTRINKCPDFSTTGKWEVTTEKNSKKETAVFDAVMICSGHHVYPHLPKDSFPGLNRFKGKCFHSRDYKEPGTWKGKRVLVIGLGNSGCDIAAELSHVAQQVIISSRSGSWVMSRVWNDGYPWDMVVITRFQTFLKNNLPTAISDWWYMKQMNARFKHENYGLMPLNGTLRKEPVFNDELPARILCGTVSIKPNVKEFTETSAVFEDGTVFEGIDCVIFATGYGYAYPFLDDSIIKSRNNEVTLYKGIFPPQLEKPTMAVIGLVQSLGAAIPTTDLQARWAAQVIRGTCILPSVNDMMDDIDEKMGKKLKWFGNSTTIQTDYIVYMDELASFIGAKPNILWLFLKDPRLAIEVFFGPCSPYQFRLVGPGKWSGARNAILTQWDRSLKPMKTRVVGGIQKPCLYSHFLRLLAVPVLIALFLVLI

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0G2JSI0A0A0G2JSI0_RATFmo3531

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict501in Ref. 2; AAH87008

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF286595
EMBL· GenBank· DDBJ
AAG44891.1
EMBL· GenBank· DDBJ
mRNA
BC087008
EMBL· GenBank· DDBJ
AAH87008.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp