Q9EPZ7 · SO1C1_RAT
- ProteinSolute carrier organic anion transporter family member 1C1
- GeneSlco1c1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids716 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Mediates the Na+-independent high affinity transport of thyroid hormones at the plasma membrane of brain capillary endothelial cells (PubMed:12923172, PubMed:17667996, PubMed:19819953).
The transport activity of substrates L-thyroxine (T4) and 3,3',5'-triiodo-L-thyronine (reverse T3, rT3) is much greater than that of 3,3',5-triiodo-L-thyronine (T3) (PubMed:12923172, PubMed:17667996, PubMed:19819953).
The prehormone, T4, is the major form in the circulating blood and is converted to the active form, T3, by the iodothyronine-deiodinase in peripheral organs (PubMed:12923172).
T3 plays an essential role in brain development via binding to specific nuclear receptors (thyroid hormone receptor) (PubMed:12923172).
Also transports organic anions such as the conjugated steroid 17-beta-glucuronosyl estradiol (17beta-estradiol 17-O-(beta-D-glucuronate)) (PubMed:12923172, PubMed:17667996, PubMed:19819953).
Transports T4 and estrone-3-sulfate in a pH-insensitive manner (By similarity).
May serve as a drug efflux system at the blood brain barrier (PubMed:12923172, PubMed:17667996).
The transport activity of substrates L-thyroxine (T4) and 3,3',5'-triiodo-L-thyronine (reverse T3, rT3) is much greater than that of 3,3',5-triiodo-L-thyronine (T3) (PubMed:12923172, PubMed:17667996, PubMed:19819953).
The prehormone, T4, is the major form in the circulating blood and is converted to the active form, T3, by the iodothyronine-deiodinase in peripheral organs (PubMed:12923172).
T3 plays an essential role in brain development via binding to specific nuclear receptors (thyroid hormone receptor) (PubMed:12923172).
Also transports organic anions such as the conjugated steroid 17-beta-glucuronosyl estradiol (17beta-estradiol 17-O-(beta-D-glucuronate)) (PubMed:12923172, PubMed:17667996, PubMed:19819953).
Transports T4 and estrone-3-sulfate in a pH-insensitive manner (By similarity).
May serve as a drug efflux system at the blood brain barrier (PubMed:12923172, PubMed:17667996).
Catalytic activity
- 3,3',5'-triiodo-L-thyronine(out) = 3,3',5'-triiodo-L-thyronine(in)
- L-thyroxine(out) = L-thyroxine(in)
- L-thyroxine sulfate(out) = L-thyroxine sulfate(in)
- 17beta-estradiol 17-O-(beta-D-glucuronate)(out) = 17beta-estradiol 17-O-(beta-D-glucuronate)(in)
- 3,3',5-triiodo-L-thyronine(out) = 3,3',5-triiodo-L-thyronine(in)
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.18 μM | L-thyroxine | |||||
0.72 μM | L-thyroxine | |||||
10.3 nM | L-thyroxine | |||||
10.7 μM | 17beta-estradiol 17-O-(beta-D-glucuronate) | |||||
6.1 μM | 17beta-estradiol 17-O-(beta-D-glucuronate) | |||||
1.3 μM | 17beta-estradiol 17-O-(beta-D-glucuronate) | |||||
0.76 μM | troglitazone sulfate | |||||
1.34 μM | cerivastatin |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
32.1 pmol/min/mg | |||||
67 pmol/min/mg | |||||
93.4 pmol/min/mg | |||||
73 pmol/min/mg | |||||
14.5 pmol/min/mg | |||||
69 pmol/min/mg |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | basolateral plasma membrane | |
Cellular Component | plasma membrane | |
Molecular Function | bile acid transmembrane transporter activity | |
Molecular Function | organic anion transmembrane transporter activity | |
Molecular Function | sodium-independent organic anion transmembrane transporter activity | |
Molecular Function | thyroid hormone transmembrane transporter activity | |
Biological Process | bile acid and bile salt transport | |
Biological Process | monoatomic ion transport | |
Biological Process | positive regulation of thyroid hormone generation | |
Biological Process | sodium-independent organic anion transport | |
Biological Process | thyroid hormone transport | |
Biological Process | transmembrane transport |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameSolute carrier organic anion transporter family member 1C1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ9EPZ7
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Note: Expressed in the plasma membranes of brain capillary endothelial cells.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-43 | Cytoplasmic | ||||
Sequence: MDTSSKENAHLFHKNSAQPAGGPSFKAGYPSTEEARPCCGKLK | ||||||
Transmembrane | 44-63 | Helical; Name=1 | ||||
Sequence: VFLGALSFVYFAKALTEGYL | ||||||
Topological domain | 64-82 | Extracellular | ||||
Sequence: KSTITQIERRFDIPSSLVG | ||||||
Transmembrane | 83-103 | Helical; Name=2 | ||||
Sequence: IIDGSFEIGNLLVITFVSYFG | ||||||
Topological domain | 104-109 | Cytoplasmic | ||||
Sequence: AKLHRP | ||||||
Transmembrane | 110-134 | Helical; Name=3 | ||||
Sequence: KIIGAGCLVMGFGTMLIAVPQFFME | ||||||
Topological domain | 135-187 | Extracellular | ||||
Sequence: KYSYEKYERYSPSSNLTPNISPCYLESSSPSPRSIVGKSQNKINDECEVDTSS | ||||||
Transmembrane | 188-216 | Helical; Name=4 | ||||
Sequence: SMWVYVFLGNLLRGLGETPIQPLGIAYLD | ||||||
Topological domain | 217-235 | Cytoplasmic | ||||
Sequence: DFASEDNAAFYIGCVQTVA | ||||||
Transmembrane | 236-256 | Helical; Name=5 | ||||
Sequence: IIGPIFGFLLGSLCAKLYVDI | ||||||
Topological domain | 257-274 | Extracellular | ||||
Sequence: GFVNLDHITITPKDPQWV | ||||||
Transmembrane | 275-299 | Helical; Name=6 | ||||
Sequence: GAWWLGYLIAGFLSLLAAVPFWCLP | ||||||
Topological domain | 300-352 | Cytoplasmic | ||||
Sequence: KTLPRSQSREDSGSSSEKSKFITDDPVNYQMAPREESMKIMEMARDFLPSLKS | ||||||
Transmembrane | 353-374 | Helical; Name=7 | ||||
Sequence: LFRNPVYILYLCASTVQFNSLF | ||||||
Topological domain | 375-394 | Extracellular | ||||
Sequence: GMVTYKPKYIEQQYGQSSSK | ||||||
Transmembrane | 395-418 | Helical; Name=8 | ||||
Sequence: ANFVIGLINIPAVALGIFSGGIVM | ||||||
Topological domain | 419-422 | Cytoplasmic | ||||
Sequence: KKFR | ||||||
Transmembrane | 423-446 | Helical; Name=9 | ||||
Sequence: LGICEATKLYLGSSVFGYLLFLSL | ||||||
Topological domain | 447-558 | Extracellular | ||||
Sequence: FALGCENSSVAGLTVSYQGTKPVSYHERALFSDCNSRCKCSDSKWEPMCGDNGITYASACLAGCQSSSRSGKNIIFSNCTCVGFAAPKSGNWSGMMGRCQKDNGCSQMFLYF | ||||||
Transmembrane | 559-581 | Helical; Name=10 | ||||
Sequence: LVISVITSYTLSLGGIPGYILLL | ||||||
Topological domain | 582-590 | Cytoplasmic | ||||
Sequence: RCIQPQLKS | ||||||
Transmembrane | 591-616 | Helical; Name=11 | ||||
Sequence: FALGIYTLAVRVLAGIPAPVYFGVLI | ||||||
Topological domain | 617-650 | Extracellular | ||||
Sequence: DTSCLKWGFKKCGSRGSCRLYDSHAFRHIYLGLT | ||||||
Transmembrane | 651-668 | Helical; Name=12 | ||||
Sequence: TLLGTVSVFLSTAVLLVL | ||||||
Topological domain | 669-716 | Cytoplasmic | ||||
Sequence: KKKYVSKRSSFITAREKIVMSSSVKKETCAARDHGLQPKYWPGKETRL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000191057 | 1-716 | Solute carrier organic anion transporter family member 1C1 | |||
Sequence: MDTSSKENAHLFHKNSAQPAGGPSFKAGYPSTEEARPCCGKLKVFLGALSFVYFAKALTEGYLKSTITQIERRFDIPSSLVGIIDGSFEIGNLLVITFVSYFGAKLHRPKIIGAGCLVMGFGTMLIAVPQFFMEKYSYEKYERYSPSSNLTPNISPCYLESSSPSPRSIVGKSQNKINDECEVDTSSSMWVYVFLGNLLRGLGETPIQPLGIAYLDDFASEDNAAFYIGCVQTVAIIGPIFGFLLGSLCAKLYVDIGFVNLDHITITPKDPQWVGAWWLGYLIAGFLSLLAAVPFWCLPKTLPRSQSREDSGSSSEKSKFITDDPVNYQMAPREESMKIMEMARDFLPSLKSLFRNPVYILYLCASTVQFNSLFGMVTYKPKYIEQQYGQSSSKANFVIGLINIPAVALGIFSGGIVMKKFRLGICEATKLYLGSSVFGYLLFLSLFALGCENSSVAGLTVSYQGTKPVSYHERALFSDCNSRCKCSDSKWEPMCGDNGITYASACLAGCQSSSRSGKNIIFSNCTCVGFAAPKSGNWSGMMGRCQKDNGCSQMFLYFLVISVITSYTLSLGGIPGYILLLRCIQPQLKSFALGIYTLAVRVLAGIPAPVYFGVLIDTSCLKWGFKKCGSRGSCRLYDSHAFRHIYLGLTTLLGTVSVFLSTAVLLVLKKKYVSKRSSFITAREKIVMSSSVKKETCAARDHGLQPKYWPGKETRL | ||||||
Glycosylation | 453 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 480↔510 | |||||
Sequence: CNSRCKCSDSKWEPMCGDNGITYASACLAGC | ||||||
Disulfide bond | 486↔506 | |||||
Sequence: CSDSKWEPMCGDNGITYASAC | ||||||
Disulfide bond | 495↔527 | |||||
Sequence: CGDNGITYASACLAGCQSSSRSGKNIIFSNCTC | ||||||
Glycosylation | 524 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 537 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Highly expressed in cerebral microvessels and choroid plexus (at protein level) (PubMed:12923172, PubMed:17667996, PubMed:18687783).
Brain-specific (PubMed:12923172, PubMed:17667996).
Not detected in glial cells, kidney, heart, lung, skeletal muscle, spleen, liver, nor testis (PubMed:12923172, PubMed:17667996).
Brain-specific (PubMed:12923172, PubMed:17667996).
Not detected in glial cells, kidney, heart, lung, skeletal muscle, spleen, liver, nor testis (PubMed:12923172, PubMed:17667996).
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-26 | Disordered | ||||
Sequence: MDTSSKENAHLFHKNSAQPAGGPSFK | ||||||
Domain | 474-529 | Kazal-like | ||||
Sequence: RALFSDCNSRCKCSDSKWEPMCGDNGITYASACLAGCQSSSRSGKNIIFSNCTCVG |
Sequence similarities
Belongs to the organo anion transporter (TC 2.A.60) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length716
- Mass (Da)78,627
- Last updated2001-03-01 v1
- Checksum487734B82A50A6EA
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I6GJ93 | A0A8I6GJ93_RAT | Slco1c1 | 667 | ||
G3V795 | G3V795_RAT | Slco1c1 | 716 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF306546 EMBL· GenBank· DDBJ | AAG37066.1 EMBL· GenBank· DDBJ | mRNA |