Q9E005 · L_ANDV

Function

function

RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs (Probable). These short capped RNAs are then used as primers for viral transcription. Cleaves ssRNA substrates but not DNA (PubMed:27300328).
Seems to downregulate the expression of its own and heterologous mRNAs through its endonuclease activity (PubMed:23576516).

Miscellaneous

Classified as His+ endonuclease since it has a histidine upstream of the active site that coordinates the first cation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: For endonuclease activity. Binds 2 Mn2+ ions in the active site. The divalent metal ions are crucial for catalytic activity (PubMed:31948728).
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: For polymerase activity.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site36Mn2+ 1 (UniProtKB | ChEBI)
Binding site54Mn2+ 2 (UniProtKB | ChEBI)
Binding site97Mn2+ 1 (UniProtKB | ChEBI)
Binding site97Mn2+ 2 (UniProtKB | ChEBI)
Binding site110Mn2+ 1 (UniProtKB | ChEBI)
Binding site111Mn2+ 1 (UniProtKB | ChEBI)
Active site124For endonuclease activity
Binding site1100Mg2+ (UniProtKB | ChEBI); catalytic; for RdRp activity

GO annotations

AspectTerm
Cellular Componenthost cell perinuclear region of cytoplasm
Molecular Functionendonuclease activity
Molecular Functionmetal ion binding
Molecular Functionnucleotide binding
Molecular FunctionRNA-dependent RNA polymerase activity
Biological Processcap snatching
Biological ProcessDNA-templated transcription
Biological Processviral RNA genome replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA-directed RNA polymerase L
  • EC number
  • Short names
    Protein L
  • Alternative names
    • Large structural protein
    • RdRp
    • Replicase
    • Transcriptase

Including 1 domain:

  • Recommended name
    cap-snatching endonuclease
  • EC number

Organism names

Accessions

  • Primary accession
    Q9E005

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis35Almost complete loss of cap-snatching endonuclease activity.
Mutagenesis36Complete loss of cap-snatching endonuclease activity.
Mutagenesis4080% loss of cap-snatching endonuclease activity.
Mutagenesis4370% loss of cap-snatching endonuclease activity.
Mutagenesis4460% loss of cap-snatching endonuclease activity.
Mutagenesis50Strongly reduced cap-snatching endonuclease activity.
Mutagenesis96Strongly reduced cap-snatching endonuclease activity.
Mutagenesis97Complete loss of cap-snatching endonuclease activity.
Mutagenesis9860% loss of cap-snatching endonuclease activity.
Mutagenesis110Complete loss of cap-snatching endonuclease activity.
Mutagenesis124Almost complete loss of cap-snatching endonuclease activity.
Mutagenesis127Almost complete loss of cap-snatching endonuclease activity.
Mutagenesis167No effect on cap-snatching endonuclease activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004551901-2153RNA-directed RNA polymerase L

Interaction

Subunit

Interacts with the viral nucleoprotein.

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain957-1143RdRp catalytic

Domain

The N-terminus contains the endonuclease activity (endoN) (PubMed:27300328).
The central region contains the RdRp activity (By similarity).
The C-terminus contains the cap-binding region (By similarity).

Sequence similarities

Belongs to the Bunyavirales RNA polymerase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,153
  • Mass (Da)
    246,857
  • Last updated
    2002-03-01 v4
  • Checksum
    84445F7EE20436EB
MEKYREIHQRVRDLAPGTVSALECIDLLDRLYAVRHDLVDQMIKHDWSDNKDVERPIGQVLLMAGIPNDIIQGMEKKIIPNSPSGQVLKSFFRMTPDNYKITGNLIEFIEVTVTADVSRGIREKKIKYEGGLQFVEHLLETESRKGNIPQPYKITFSVVAVKTDGSNISTQWPSRRNDGVVQHMRLVQADINYVREHLIKLDERASLEAMFNLKFHVSGPKLRYFNIPDYRPQQLCEPRIDNLIQYCKNWLTKEHKFVFKEVSGANVIQAFESHEQLHLQKYNESRKPRNFLLLQLTVQGAYLPSTISSDQCNTRIGCLEISKNQPETPVQMLALDISYKYLSLTRDELINYYSPRVHFQSSPNVKEPGTLKLGLSQLNPLSKSILDNVGKHKKDKGLFGEIIDSINVASQIQINACAKIIEQILSNLEINIGEINASMPSPNKTTGVDDLLNKFYDNELGKYMLSILRKTAAWHIGHLVRDITESLIAHAGLRRSKYWSVHAYDHGNVILFILPSKSLEVVGSYIRYFTVFKDGIGLIDADNIDSKAEIDGVTWCYSKVMSIDLNRLLALNIAFEKSLLATATWFQYYTEDQGHFPLQHALRSIFSFHFLLCVSQKMKLCAIFDNLRYLIPSVTSLYSGYELLIEKFFERPFKSSLDVYLYSIIKSLLISLAQNNKVRFYSRVRLLGLTVDHSTVGASGVYPSLMSRVVYKHYRSLISEATTCFFLFEKGLHGNLPEEAKIHLETIEWARKFQEKEKQYGDILLKEGYTIESVINGEVDVEQQLFCQEVSELSAQELNKYLQAKSQVLCANIMNKHWDKPYFSQTRNISLKGMSGALQEDGHLAASVTLIEAIRFLNRSQTNPNVIDMYEQTKQSKAQARIVRKYQRTEADRGFFITTLPTRVRLEIIEDYFDAIAKVVPEEYISYGGDKKVLNIQNALEKALRWASGVSEITTSTGKSIKFKRKLMYVSADATKWSPGDNSAKFRRFTQAIYDGLSDNKLKCCVVDALRNIYETEFFMSRKLHRYIDSMENHSDAVEDFLAFFSNGVSANVKGNWLQGNLNKCSSLFGAAVSLLFREVWKQLFPELECFFEFAHHSDDALFIYGYLEPEDDGTDWFLYVSQQIQAGNFHWHAINQEMWKSMFNLHEHLLLMGSIKVSPKKTTVSPTNAEFLSTFFEGCAVSIPFVKILLGSLSDLPGLGFFDDLAAAQSRCVKSLDLGACPQLAQLAIVLCTSKVERLYGTADGMVNSPTAFLKVNKAHVPVPLGGDGSMSIMELATAGFGMADKNILKNAFISYKHTRRDGDRYVLGLFKFLMSLSEDVFQHDRLGEFSFVGKVQWKVFTPKAEFEFHDQFSHNYLLEWTRQHPVYDYIIPRNRDNLLVYLVRKLNDPSIITAMTMQSPLQLRFRMQAKQHMKVCRYEGEWVTFREVLAAADSFATSYQPTERDMDLFNTLVSCTFSKEYAWKDFLNEVRCEVLTTRHVHRPKIARTFTVREKDQAIQNPINSVIGYKYALTVDEVSDVLDSAFFPESLSADLQVMKDGVYRELGLDISSPEVLKRIAPLLYKAGRSRVVIVEGNVEGTAESICSYWLKTMSLIKTIRVRPKKEVLKAMSLYSVKENIGLQDDIAATRLCIEIWRWCKANEQDVKEWLTSLYFEKQTLMDWVERFRRKGVVPIDPEIQCIGLLLYDVLGYKSVLQMQANRRAYSGKQYDAYCVQTYNEETKLYEGDLRVTFNFGLDCARLEVFWDKKEYILETSITQRHVLRLLMEEVSQELIRCGMRFKTEQVNQTRSLVLFKTEAGFEWGKPNVPCIVYKHCVLRTGLRTKQPINKEFMINVQSDGFRAIAQMDIESPRFLLAHAYHTLRDIRYQAVQAVGNVWFKTEQHKLFINPIISSGLLENFMKGLPAAIPPAAYSLIMNKAKISVDLFMFNELLALINRNNILNLDGIEETSEGYSTVTSMSSKQWSEEMSLMSDDDIDDMEDFTIALDDIDFEQINLEEDIQHFLQDESAYVGDLLIQTEDIEVKKIRGVTRVLEPVKLLKSWVSKGLAIDKVYNPIGIILMARYMSKTYNFSSTPLALLNPYDLTELESVVKGWGETVNDRFKDLDIEAQTVVKEKGVQPEDVLPDSLFSFRHVDVLLRRLFPRDPVSTFY

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF291704
EMBL· GenBank· DDBJ
AAG22533.4
EMBL· GenBank· DDBJ
Genomic RNA

Genome annotation databases

Similar Proteins

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