Q9E005 · L_ANDV
- ProteinRNA-directed RNA polymerase L
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids2153 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs (Probable). These short capped RNAs are then used as primers for viral transcription. Cleaves ssRNA substrates but not DNA (PubMed:27300328).
Seems to downregulate the expression of its own and heterologous mRNAs through its endonuclease activity (PubMed:23576516).
Seems to downregulate the expression of its own and heterologous mRNAs through its endonuclease activity (PubMed:23576516).
Miscellaneous
Classified as His+ endonuclease since it has a histidine upstream of the active site that coordinates the first cation.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
Cofactor
Protein has several cofactor binding sites:
Note: For endonuclease activity. Binds 2 Mn2+ ions in the active site. The divalent metal ions are crucial for catalytic activity (PubMed:31948728).
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: For polymerase activity.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 36 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 54 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 97 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 97 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 110 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 111 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Active site | 124 | For endonuclease activity | ||||
Sequence: K | ||||||
Binding site | 1100 | Mg2+ (UniProtKB | ChEBI); catalytic; for RdRp activity | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell perinuclear region of cytoplasm | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Molecular Function | RNA-dependent RNA polymerase activity | |
Biological Process | cap snatching | |
Biological Process | DNA-templated transcription | |
Biological Process | viral RNA genome replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRNA-directed RNA polymerase L
- EC number
- Short namesProtein L
- Alternative names
Including 1 domain:
- Recommended namecap-snatching endonuclease
- EC number
Organism names
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Negarnaviricota > Polyploviricotina > Ellioviricetes > Bunyavirales > Hantaviridae > Mammantavirinae > Orthohantavirus
- Virus hosts
Accessions
- Primary accessionQ9E005
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 35 | Almost complete loss of cap-snatching endonuclease activity. | ||||
Sequence: R → H | ||||||
Mutagenesis | 36 | Complete loss of cap-snatching endonuclease activity. | ||||
Sequence: H → R | ||||||
Mutagenesis | 40 | 80% loss of cap-snatching endonuclease activity. | ||||
Sequence: D → E | ||||||
Mutagenesis | 43 | 70% loss of cap-snatching endonuclease activity. | ||||
Sequence: I → A | ||||||
Mutagenesis | 44 | 60% loss of cap-snatching endonuclease activity. | ||||
Sequence: K → A | ||||||
Mutagenesis | 50 | Strongly reduced cap-snatching endonuclease activity. | ||||
Sequence: N → A | ||||||
Mutagenesis | 96 | Strongly reduced cap-snatching endonuclease activity. | ||||
Sequence: P → A | ||||||
Mutagenesis | 97 | Complete loss of cap-snatching endonuclease activity. | ||||
Sequence: D → E | ||||||
Mutagenesis | 98 | 60% loss of cap-snatching endonuclease activity. | ||||
Sequence: N → A | ||||||
Mutagenesis | 110 | Complete loss of cap-snatching endonuclease activity. | ||||
Sequence: E → A | ||||||
Mutagenesis | 124 | Almost complete loss of cap-snatching endonuclease activity. | ||||
Sequence: K → A | ||||||
Mutagenesis | 127 | Almost complete loss of cap-snatching endonuclease activity. | ||||
Sequence: K → A | ||||||
Mutagenesis | 167 | No effect on cap-snatching endonuclease activity. | ||||
Sequence: N → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000455190 | 1-2153 | RNA-directed RNA polymerase L | |||
Sequence: MEKYREIHQRVRDLAPGTVSALECIDLLDRLYAVRHDLVDQMIKHDWSDNKDVERPIGQVLLMAGIPNDIIQGMEKKIIPNSPSGQVLKSFFRMTPDNYKITGNLIEFIEVTVTADVSRGIREKKIKYEGGLQFVEHLLETESRKGNIPQPYKITFSVVAVKTDGSNISTQWPSRRNDGVVQHMRLVQADINYVREHLIKLDERASLEAMFNLKFHVSGPKLRYFNIPDYRPQQLCEPRIDNLIQYCKNWLTKEHKFVFKEVSGANVIQAFESHEQLHLQKYNESRKPRNFLLLQLTVQGAYLPSTISSDQCNTRIGCLEISKNQPETPVQMLALDISYKYLSLTRDELINYYSPRVHFQSSPNVKEPGTLKLGLSQLNPLSKSILDNVGKHKKDKGLFGEIIDSINVASQIQINACAKIIEQILSNLEINIGEINASMPSPNKTTGVDDLLNKFYDNELGKYMLSILRKTAAWHIGHLVRDITESLIAHAGLRRSKYWSVHAYDHGNVILFILPSKSLEVVGSYIRYFTVFKDGIGLIDADNIDSKAEIDGVTWCYSKVMSIDLNRLLALNIAFEKSLLATATWFQYYTEDQGHFPLQHALRSIFSFHFLLCVSQKMKLCAIFDNLRYLIPSVTSLYSGYELLIEKFFERPFKSSLDVYLYSIIKSLLISLAQNNKVRFYSRVRLLGLTVDHSTVGASGVYPSLMSRVVYKHYRSLISEATTCFFLFEKGLHGNLPEEAKIHLETIEWARKFQEKEKQYGDILLKEGYTIESVINGEVDVEQQLFCQEVSELSAQELNKYLQAKSQVLCANIMNKHWDKPYFSQTRNISLKGMSGALQEDGHLAASVTLIEAIRFLNRSQTNPNVIDMYEQTKQSKAQARIVRKYQRTEADRGFFITTLPTRVRLEIIEDYFDAIAKVVPEEYISYGGDKKVLNIQNALEKALRWASGVSEITTSTGKSIKFKRKLMYVSADATKWSPGDNSAKFRRFTQAIYDGLSDNKLKCCVVDALRNIYETEFFMSRKLHRYIDSMENHSDAVEDFLAFFSNGVSANVKGNWLQGNLNKCSSLFGAAVSLLFREVWKQLFPELECFFEFAHHSDDALFIYGYLEPEDDGTDWFLYVSQQIQAGNFHWHAINQEMWKSMFNLHEHLLLMGSIKVSPKKTTVSPTNAEFLSTFFEGCAVSIPFVKILLGSLSDLPGLGFFDDLAAAQSRCVKSLDLGACPQLAQLAIVLCTSKVERLYGTADGMVNSPTAFLKVNKAHVPVPLGGDGSMSIMELATAGFGMADKNILKNAFISYKHTRRDGDRYVLGLFKFLMSLSEDVFQHDRLGEFSFVGKVQWKVFTPKAEFEFHDQFSHNYLLEWTRQHPVYDYIIPRNRDNLLVYLVRKLNDPSIITAMTMQSPLQLRFRMQAKQHMKVCRYEGEWVTFREVLAAADSFATSYQPTERDMDLFNTLVSCTFSKEYAWKDFLNEVRCEVLTTRHVHRPKIARTFTVREKDQAIQNPINSVIGYKYALTVDEVSDVLDSAFFPESLSADLQVMKDGVYRELGLDISSPEVLKRIAPLLYKAGRSRVVIVEGNVEGTAESICSYWLKTMSLIKTIRVRPKKEVLKAMSLYSVKENIGLQDDIAATRLCIEIWRWCKANEQDVKEWLTSLYFEKQTLMDWVERFRRKGVVPIDPEIQCIGLLLYDVLGYKSVLQMQANRRAYSGKQYDAYCVQTYNEETKLYEGDLRVTFNFGLDCARLEVFWDKKEYILETSITQRHVLRLLMEEVSQELIRCGMRFKTEQVNQTRSLVLFKTEAGFEWGKPNVPCIVYKHCVLRTGLRTKQPINKEFMINVQSDGFRAIAQMDIESPRFLLAHAYHTLRDIRYQAVQAVGNVWFKTEQHKLFINPIISSGLLENFMKGLPAAIPPAAYSLIMNKAKISVDLFMFNELLALINRNNILNLDGIEETSEGYSTVTSMSSKQWSEEMSLMSDDDIDDMEDFTIALDDIDFEQINLEEDIQHFLQDESAYVGDLLIQTEDIEVKKIRGVTRVLEPVKLLKSWVSKGLAIDKVYNPIGIILMARYMSKTYNFSSTPLALLNPYDLTELESVVKGWGETVNDRFKDLDIEAQTVVKEKGVQPEDVLPDSLFSFRHVDVLLRRLFPRDPVSTFY |
Interaction
Subunit
Interacts with the viral nucleoprotein.
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 957-1143 | RdRp catalytic | ||||
Sequence: TGKSIKFKRKLMYVSADATKWSPGDNSAKFRRFTQAIYDGLSDNKLKCCVVDALRNIYETEFFMSRKLHRYIDSMENHSDAVEDFLAFFSNGVSANVKGNWLQGNLNKCSSLFGAAVSLLFREVWKQLFPELECFFEFAHHSDDALFIYGYLEPEDDGTDWFLYVSQQIQAGNFHWHAINQEMWKSM |
Domain
The N-terminus contains the endonuclease activity (endoN) (PubMed:27300328).
The central region contains the RdRp activity (By similarity).
The C-terminus contains the cap-binding region (By similarity).
The central region contains the RdRp activity (By similarity).
The C-terminus contains the cap-binding region (By similarity).
Sequence similarities
Belongs to the Bunyavirales RNA polymerase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,153
- Mass (Da)246,857
- Last updated2002-03-01 v4
- Checksum84445F7EE20436EB
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF291704 EMBL· GenBank· DDBJ | AAG22533.4 EMBL· GenBank· DDBJ | Genomic RNA |