Q9DDT5 · SPT5H_DANRE

Function

function

May function as a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates transcription elongation by RNA polymerase II. Probably enhances transcriptional pausing at sites proximal to the promoter, which may facilitate the assembly of an elongation competent RNA polymerase II complex. Also acts to stimulate transcriptional elongation at low nucleotide concentrations. Regulation of transcriptional elongation by this protein is required for the expression of genes which control neuronal development.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular ComponentDSIF complex
Cellular Componentnucleus
Molecular FunctionDNA binding
Molecular FunctionmRNA binding
Biological Processcentral nervous system neuron development
Biological Processhemopoiesis
Biological Processnegative regulation of fibroblast growth factor receptor signaling pathway
Biological Processnegative regulation of transcription elongation by RNA polymerase II
Biological Processneuron migration
Biological Processpositive regulation of hematopoietic progenitor cell differentiation
Biological Processpositive regulation of hematopoietic stem cell differentiation
Biological Processpositive regulation of receptor signaling pathway via JAK-STAT
Biological Processpositive regulation of transcription elongation by RNA polymerase II
Biological Processpositive regulation of type II interferon-mediated signaling pathway
Biological Processregulation of transcription by RNA polymerase II
Biological Processtranscription elongation by RNA polymerase II
Biological Processtranscription elongation-coupled chromatin remodeling

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Transcription elongation factor SPT5
  • Alternative names
    • DRB sensitivity-inducing factor large subunit (DSIF large subunit)
    • Protein foggy

Gene names

    • Name
      supt5h
    • Synonyms
      fog, spt5

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio

Accessions

  • Primary accession
    Q9DDT5
  • Secondary accessions
    • Q6PFJ3

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis1012In fog(m806); reduced development of dopamine containing neurons in the hypothalamus. Specifically abrogates inhibition of transcriptional elongation. No effect on the stimulation of transcriptional elongation at low nucleotide concentrations.

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002084721-1084Transcription elongation factor SPT5
Modified residue665Phosphoserine
Modified residue773Phosphothreonine; by CDK9
Modified residue782Phosphothreonine; by CDK9

Post-translational modification

Phosphorylated. Phosphorylation by P-TEFb alleviates transcriptional pausing (By similarity).

Keywords

Proteomic databases

PTM databases

Expression

Developmental stage

Maternally expressed throughout the early blastoderm. Expressed in the neural plate of the tailbud stage embryo, at 10 hours post-fertilization (hpf). Highly expressed in the developing brain at 28 hpf, and at lower levels in the rest of the embryo.

Interaction

Subunit

Interacts with SUPT4H1 to form the DSIF complex. DSIF interacts with RNA polymerase II and with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of CDK9 and cyclin-T (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain, repeat.

TypeIDPosition(s)Description
Region1-91Disordered
Compositional bias42-60Acidic residues
Compositional bias61-78Basic and acidic residues
Region175-269Interaction with SUPT4H1
Domain272-305KOW 1
Region312-419Interaction with RNA polymerase II
Domain419-450KOW 2
Domain471-502KOW 3
Domain593-626KOW 4
Region667-700Disordered
Domain702-735KOW 5
Compositional bias748-829Polar residues
Region748-973Disordered
Repeat758-763CTR1-1; approximate
Region758-8158 X 7 AA approximate tandem repeats of G-S-[QR]-T-P-X-[YQ], motif CTR1
Repeat764-769CTR1-2; approximate
Repeat770-776CTR1-3
Repeat779-785CTR1-4
Repeat786-792CTR1-5
Repeat794-800CTR1-6
Repeat801-807CTR1-7
Repeat809-815CTR1-8
Repeat842-849CTR2-1
Region842-94810 X 8 AA approximate tandem repeats of P-[TS]-P-S-P-[QA]-[SG]-Y, motif CTR2
Repeat852-860CTR2-2; approximate
Compositional bias857-873Pro residues
Repeat861-867CTR2-3; approximate
Compositional bias874-945Polar residues
Repeat879-883CTR2-4; half-length
Repeat894-900CTR2-5; approximate
Repeat902-909CTR2-6
Repeat914-919CTR2-7; approximate
Repeat922-928CTR2-8
Repeat930-937CTR2-9
Repeat941-948CTR2-10
Compositional bias946-960Pro residues

Sequence similarities

Belongs to the SPT5 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q9DDT5-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    1,084
  • Mass (Da)
    120,379
  • Last updated
    2001-03-01 v1
  • Checksum
    467C60789E1A57AB
MSDSEDSDFSDNQSERSSEAEEVEENEEEEEQGSVAGSDKAEEEGEDLEDEEEYDEEEEEDDDRPRKKARHGGFILDEADVDDEYEDEDPWEDGAEDILEKEEAEVSNLDHVVLDEDHSGSRRLQNLWRDSREEALGEYYMRKYAKSSGGEHFYGGSEDLSDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFVAYQCTDTPLQIKSVVAPEHVKGYIYVEAYKQTHVKAAIEGVGNLRMGFWNQQMVPIKEMTDVLKVVKEVTNLKPKSWVRLKRGLYKDDIAQVDYVEPSQNTISLKMIPRIDLDRIKARMSMKDWFAKRKKFKRPPQRLFDAEKIRSLGGEVSHDGDFMIFEANRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVVTETTGKEREHNLQAGDNVEVCEGELINLQGKILSVDGNKITIMPKHEDLKDPLEFPAHELRKYFRMGDHVKVIAGRYEGDTGLIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDAGGQHEWGELVQLDPQTVGVIVRLERETFQVLNMHGKVLTVRHQAVNRRKDNRFAVALDSEQNNIHVKDIVKVIDGPHSGREGEIRHIFRGFAFLHCKKLVENGGMFVCKARHLVLAGGSKPRDVTNFTVGGFAPMSPRISSPMHPGGGGQPQRGGGGGGGGGMGRGRGRRDNDLIGQTVRISQGPYKGYIGVVKDATESTARVELHSTCQTISVDRQRLTTVGGKERQGRSSTHLRTPMYGSQTPIYGTGSRTPMYGSQTPLHDGSRTPHYGSQTPLHDGSRTPGQSGAWDPNNPNTPSRPDDEYEFAYDDEPSPSPQGYGGTPNPQTPGYPEVPSPQVNPQYNPQTPGTPAMYNTDQYSPYAAPSPQGSYQPSPSPQSYHQVAPSPVGYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSNIDQASNDWVTTDIMVRVKDTFLDGGVINQTGIIRSVTGGMCSVFLQDTEKVVSISSEHLEPVTPTKNNKVKVILGEDREATGVLLSIDGEDGIVRMELDEQLKILNLRFLGKLEV

Q9DDT5-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8M9Q0J3A0A8M9Q0J3_DANREsupt5h1086
A0A8M1P6F8A0A8M1P6F8_DANREsupt5h1084

Features

Showing features for compositional bias, alternative sequence.

TypeIDPosition(s)Description
Compositional bias42-60Acidic residues
Compositional bias61-78Basic and acidic residues
Alternative sequenceVSP_016284603-830in isoform 2
Compositional bias748-829Polar residues
Compositional bias857-873Pro residues
Compositional bias874-945Polar residues
Compositional bias946-960Pro residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF288409
EMBL· GenBank· DDBJ
AAG37030.1
EMBL· GenBank· DDBJ
mRNA
BC057529
EMBL· GenBank· DDBJ
AAH57529.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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