Q9DDT5 · SPT5H_DANRE
- ProteinTranscription elongation factor SPT5
- Genesupt5h
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1084 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
May function as a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates transcription elongation by RNA polymerase II. Probably enhances transcriptional pausing at sites proximal to the promoter, which may facilitate the assembly of an elongation competent RNA polymerase II complex. Also acts to stimulate transcriptional elongation at low nucleotide concentrations. Regulation of transcriptional elongation by this protein is required for the expression of genes which control neuronal development.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTranscription elongation factor SPT5
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionQ9DDT5
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 1012 | In fog(m806); reduced development of dopamine containing neurons in the hypothalamus. Specifically abrogates inhibition of transcriptional elongation. No effect on the stimulation of transcriptional elongation at low nucleotide concentrations. | ||||
Sequence: V → D |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000208472 | 1-1084 | Transcription elongation factor SPT5 | |||
Sequence: MSDSEDSDFSDNQSERSSEAEEVEENEEEEEQGSVAGSDKAEEEGEDLEDEEEYDEEEEEDDDRPRKKARHGGFILDEADVDDEYEDEDPWEDGAEDILEKEEAEVSNLDHVVLDEDHSGSRRLQNLWRDSREEALGEYYMRKYAKSSGGEHFYGGSEDLSDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFVAYQCTDTPLQIKSVVAPEHVKGYIYVEAYKQTHVKAAIEGVGNLRMGFWNQQMVPIKEMTDVLKVVKEVTNLKPKSWVRLKRGLYKDDIAQVDYVEPSQNTISLKMIPRIDLDRIKARMSMKDWFAKRKKFKRPPQRLFDAEKIRSLGGEVSHDGDFMIFEANRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVVTETTGKEREHNLQAGDNVEVCEGELINLQGKILSVDGNKITIMPKHEDLKDPLEFPAHELRKYFRMGDHVKVIAGRYEGDTGLIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDAGGQHEWGELVQLDPQTVGVIVRLERETFQVLNMHGKVLTVRHQAVNRRKDNRFAVALDSEQNNIHVKDIVKVIDGPHSGREGEIRHIFRGFAFLHCKKLVENGGMFVCKARHLVLAGGSKPRDVTNFTVGGFAPMSPRISSPMHPGGGGQPQRGGGGGGGGGMGRGRGRRDNDLIGQTVRISQGPYKGYIGVVKDATESTARVELHSTCQTISVDRQRLTTVGGKERQGRSSTHLRTPMYGSQTPIYGTGSRTPMYGSQTPLHDGSRTPHYGSQTPLHDGSRTPGQSGAWDPNNPNTPSRPDDEYEFAYDDEPSPSPQGYGGTPNPQTPGYPEVPSPQVNPQYNPQTPGTPAMYNTDQYSPYAAPSPQGSYQPSPSPQSYHQVAPSPVGYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSNIDQASNDWVTTDIMVRVKDTFLDGGVINQTGIIRSVTGGMCSVFLQDTEKVVSISSEHLEPVTPTKNNKVKVILGEDREATGVLLSIDGEDGIVRMELDEQLKILNLRFLGKLEV | ||||||
Modified residue | 665 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 773 | Phosphothreonine; by CDK9 | ||||
Sequence: T | ||||||
Modified residue | 782 | Phosphothreonine; by CDK9 | ||||
Sequence: T |
Post-translational modification
Phosphorylated. Phosphorylation by P-TEFb alleviates transcriptional pausing (By similarity).
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Developmental stage
Maternally expressed throughout the early blastoderm. Expressed in the neural plate of the tailbud stage embryo, at 10 hours post-fertilization (hpf). Highly expressed in the developing brain at 28 hpf, and at lower levels in the rest of the embryo.
Interaction
Subunit
Interacts with SUPT4H1 to form the DSIF complex. DSIF interacts with RNA polymerase II and with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of CDK9 and cyclin-T (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-91 | Disordered | ||||
Sequence: MSDSEDSDFSDNQSERSSEAEEVEENEEEEEQGSVAGSDKAEEEGEDLEDEEEYDEEEEEDDDRPRKKARHGGFILDEADVDDEYEDEDPW | ||||||
Compositional bias | 42-60 | Acidic residues | ||||
Sequence: EEEGEDLEDEEEYDEEEEE | ||||||
Compositional bias | 61-78 | Basic and acidic residues | ||||
Sequence: DDDRPRKKARHGGFILDE | ||||||
Region | 175-269 | Interaction with SUPT4H1 | ||||
Sequence: DPNLWTVKCKIGEERATAISLMRKFVAYQCTDTPLQIKSVVAPEHVKGYIYVEAYKQTHVKAAIEGVGNLRMGFWNQQMVPIKEMTDVLKVVKEV | ||||||
Domain | 272-305 | KOW 1 | ||||
Sequence: LKPKSWVRLKRGLYKDDIAQVDYVEPSQNTISLK | ||||||
Region | 312-419 | Interaction with RNA polymerase II | ||||
Sequence: LDRIKARMSMKDWFAKRKKFKRPPQRLFDAEKIRSLGGEVSHDGDFMIFEANRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVVTETTGKEREHNL | ||||||
Domain | 419-450 | KOW 2 | ||||
Sequence: LQAGDNVEVCEGELINLQGKILSVDGNKITIM | ||||||
Domain | 471-502 | KOW 3 | ||||
Sequence: FRMGDHVKVIAGRYEGDTGLIVRVEENFVILF | ||||||
Domain | 593-626 | KOW 4 | ||||
Sequence: IHVKDIVKVIDGPHSGREGEIRHIFRGFAFLHCK | ||||||
Region | 667-700 | Disordered | ||||
Sequence: RISSPMHPGGGGQPQRGGGGGGGGGMGRGRGRRD | ||||||
Domain | 702-735 | KOW 5 | ||||
Sequence: DLIGQTVRISQGPYKGYIGVVKDATESTARVELH | ||||||
Compositional bias | 748-829 | Polar residues | ||||
Sequence: LTTVGGKERQGRSSTHLRTPMYGSQTPIYGTGSRTPMYGSQTPLHDGSRTPHYGSQTPLHDGSRTPGQSGAWDPNNPNTPSR | ||||||
Region | 748-973 | Disordered | ||||
Sequence: LTTVGGKERQGRSSTHLRTPMYGSQTPIYGTGSRTPMYGSQTPLHDGSRTPHYGSQTPLHDGSRTPGQSGAWDPNNPNTPSRPDDEYEFAYDDEPSPSPQGYGGTPNPQTPGYPEVPSPQVNPQYNPQTPGTPAMYNTDQYSPYAAPSPQGSYQPSPSPQSYHQVAPSPVGYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSNIDQA | ||||||
Repeat | 758-763 | CTR1-1; approximate | ||||
Sequence: GRSSTH | ||||||
Region | 758-815 | 8 X 7 AA approximate tandem repeats of G-S-[QR]-T-P-X-[YQ], motif CTR1 | ||||
Sequence: GRSSTHLRTPMYGSQTPIYGTGSRTPMYGSQTPLHDGSRTPHYGSQTPLHDGSRTPGQ | ||||||
Repeat | 764-769 | CTR1-2; approximate | ||||
Sequence: LRTPMY | ||||||
Repeat | 770-776 | CTR1-3 | ||||
Sequence: GSQTPIY | ||||||
Repeat | 779-785 | CTR1-4 | ||||
Sequence: GSRTPMY | ||||||
Repeat | 786-792 | CTR1-5 | ||||
Sequence: GSQTPLH | ||||||
Repeat | 794-800 | CTR1-6 | ||||
Sequence: GSRTPHY | ||||||
Repeat | 801-807 | CTR1-7 | ||||
Sequence: GSQTPLH | ||||||
Repeat | 809-815 | CTR1-8 | ||||
Sequence: GSRTPGQ | ||||||
Repeat | 842-849 | CTR2-1 | ||||
Sequence: PSPSPQGY | ||||||
Region | 842-948 | 10 X 8 AA approximate tandem repeats of P-[TS]-P-S-P-[QA]-[SG]-Y, motif CTR2 | ||||
Sequence: PSPSPQGYGGTPNPQTPGYPEVPSPQVNPQYNPQTPGTPAMYNTDQYSPYAAPSPQGSYQPSPSPQSYHQVAPSPVGYQNTHSPASYHPTPSPMAYQASPSPSPVGY | ||||||
Repeat | 852-860 | CTR2-2; approximate | ||||
Sequence: TPNPQTPGY | ||||||
Compositional bias | 857-873 | Pro residues | ||||
Sequence: TPGYPEVPSPQVNPQYN | ||||||
Repeat | 861-867 | CTR2-3; approximate | ||||
Sequence: PEVPSPQ | ||||||
Compositional bias | 874-945 | Polar residues | ||||
Sequence: PQTPGTPAMYNTDQYSPYAAPSPQGSYQPSPSPQSYHQVAPSPVGYQNTHSPASYHPTPSPMAYQASPSPSP | ||||||
Repeat | 879-883 | CTR2-4; half-length | ||||
Sequence: TPAMY | ||||||
Repeat | 894-900 | CTR2-5; approximate | ||||
Sequence: PSPQGSY | ||||||
Repeat | 902-909 | CTR2-6 | ||||
Sequence: PSPSPQSY | ||||||
Repeat | 914-919 | CTR2-7; approximate | ||||
Sequence: PSPVGY | ||||||
Repeat | 922-928 | CTR2-8 | ||||
Sequence: THSPASY | ||||||
Repeat | 930-937 | CTR2-9 | ||||
Sequence: PTPSPMAY | ||||||
Repeat | 941-948 | CTR2-10 | ||||
Sequence: PSPSPVGY | ||||||
Compositional bias | 946-960 | Pro residues | ||||
Sequence: VGYSPMTPGAPSPGG |
Sequence similarities
Belongs to the SPT5 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q9DDT5-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length1,084
- Mass (Da)120,379
- Last updated2001-03-01 v1
- Checksum467C60789E1A57AB
Q9DDT5-2
- Name2
- Differences from canonical
- 603-830: Missing
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8M9Q0J3 | A0A8M9Q0J3_DANRE | supt5h | 1086 | ||
A0A8M1P6F8 | A0A8M1P6F8_DANRE | supt5h | 1084 |
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 42-60 | Acidic residues | ||||
Sequence: EEEGEDLEDEEEYDEEEEE | ||||||
Compositional bias | 61-78 | Basic and acidic residues | ||||
Sequence: DDDRPRKKARHGGFILDE | ||||||
Alternative sequence | VSP_016284 | 603-830 | in isoform 2 | |||
Sequence: Missing | ||||||
Compositional bias | 748-829 | Polar residues | ||||
Sequence: LTTVGGKERQGRSSTHLRTPMYGSQTPIYGTGSRTPMYGSQTPLHDGSRTPHYGSQTPLHDGSRTPGQSGAWDPNNPNTPSR | ||||||
Compositional bias | 857-873 | Pro residues | ||||
Sequence: TPGYPEVPSPQVNPQYN | ||||||
Compositional bias | 874-945 | Polar residues | ||||
Sequence: PQTPGTPAMYNTDQYSPYAAPSPQGSYQPSPSPQSYHQVAPSPVGYQNTHSPASYHPTPSPMAYQASPSPSP | ||||||
Compositional bias | 946-960 | Pro residues | ||||
Sequence: VGYSPMTPGAPSPGG |
Keywords
- Coding sequence diversity
- Technical term