Q9DB34 · CHM2A_MOUSE

  • Protein
    Charged multivesicular body protein 2a
  • Gene
    Chmp2a
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2 (By similarity).
ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentamphisome membrane
Cellular Componentautophagosome membrane
Cellular Componentchromatin
Cellular Componentcytosol
Cellular ComponentESCRT III complex
Cellular Componentkinetochore
Cellular Componentkinetochore microtubule
Cellular Componentlysosomal membrane
Cellular Componentmembrane coat
Cellular Componentmidbody
Cellular Componentmultivesicular body
Cellular Componentmultivesicular body membrane
Cellular Componentnuclear envelope
Cellular Componentnuclear pore
Cellular Componentplasma membrane
Molecular Functionphosphatidylcholine binding
Molecular Functionprotein domain specific binding
Biological Processautophagosome maturation
Biological Processautophagy
Biological Processendosome transport via multivesicular body sorting pathway
Biological Processexit from mitosis
Biological Processlate endosome to lysosome transport
Biological Processlate endosome to vacuole transport
Biological Processmembrane fission
Biological Processmembrane invagination
Biological Processmidbody abscission
Biological Processmitotic metaphase chromosome alignment
Biological Processmultivesicular body assembly
Biological Processmultivesicular body sorting pathway
Biological Processmultivesicular body-lysosome fusion
Biological Processnegative regulation of centriole elongation
Biological Processnuclear membrane reassembly
Biological Processnucleus organization
Biological Processplasma membrane repair
Biological Processpositive regulation of exosomal secretion
Biological Processprotein homooligomerization
Biological Processprotein polymerization
Biological Processprotein transport
Biological Processregulation of centrosome duplication
Biological Processregulation of mitotic spindle assembly
Biological Processubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
Biological Processvesicle fusion with vacuole
Biological Processviral budding from plasma membrane
Biological Processviral budding via host ESCRT complex

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Charged multivesicular body protein 2a
  • Alternative names
    • Chromatin-modifying protein 2a (CHMP2a)
    • Vacuolar protein sorting-associated protein 2 (mVps2)

Gene names

    • Name
      Chmp2a

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q9DB34

Proteomes

Organism-specific databases

Subcellular Location

Late endosome membrane
; Peripheral membrane protein
Cytoplasm
Nucleus envelope
Note: Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body. Localizes to the reforming nuclear envelope on chromatin disks during late anaphase (By similarity).

Keywords

PTM/Processing

Features

Showing features for modified residue, chain.

TypeIDPosition(s)Description
Modified residue1N-acetylmethionine
ChainPRO_00002114631-222Charged multivesicular body protein 2a
Modified residue184Phosphoserine
Modified residue185Phosphothreonine
Modified residue188Phosphoserine
Modified residue190Phosphoserine
Modified residue203Phosphoserine

Post-translational modification

ISGylated in a CHMP5-dependent manner. Isgylation weakens and inhibits its interactions with VPS4A and VTA1 respectively (By similarity).

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Widely expressed. Highly expressed in brain, heart, liver and kidney.

Gene expression databases

Interaction

Subunit

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. In vitro, heteromerizes with CHMP3 (but not CHMP4) to form helical tubular structures that expose membrane-interacting sites on the outside whereas VPS4B can associate on the inside of the tubule. Interacts with CHMP1B, CHMP2B, CHMP3, CHMP4A, CHMP4B, CHMP4C and CHMP5. Interacts with VPS4A; the interaction is direct. Interacts with VPS4B; the interaction is direct. Interacts with MITD1. Interacts with VTA1; the interaction probably involves the open conformation of CHMP2A (By similarity).
View interactors in UniProtKB
View CPX-332 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for coiled coil, region, motif.

TypeIDPosition(s)Description
Coiled coil12-53
Region56-222Interaction with VPS4B
Region179-198Disordered
Coiled coil195-222
Motif210-220MIT-interacting motif
Region217-222Interaction with VTA1

Domain

The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.

Sequence similarities

Belongs to the SNF7 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    222
  • Mass (Da)
    25,134
  • Last updated
    2001-06-01 v1
  • Checksum
    F2B86C623832E29E
MDLLFGRRKTPEELLRQNQRALNRAMRELDRERQKLETQEKKIIADIKKMAKQGQMDAVRIMAKDLVRTRRYVRKFVLMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMGTMNRQLKLPQIQKIMMEFERQAEIMDMKEEMMNDAIDDAMGDEEDEEESDAVVSQVLDELGLSLTDELSNLPSTGGSLSVAAGGKKAEATASALADADADLEERLKNLRRD

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1B0GR63A0A1B0GR63_MOUSEChmp2a172
A0A1B0GT75A0A1B0GT75_MOUSEChmp2a162
A0A1B0GSH4A0A1B0GSH4_MOUSEChmp2a56

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK005267
EMBL· GenBank· DDBJ
BAB23919.1
EMBL· GenBank· DDBJ
mRNA
BC012230
EMBL· GenBank· DDBJ
AAH12230.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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