The authors biochemically separate the NADH dehydrogenase module from complex I of bovine heart into subunit I II and III fractions. Subunit II fraction also known as the NADH dehydrogenase (ubiquinone) flavoprotein 2 protein has a molecular weight of 24 000 Da and contains an iron-sulfur center.
The results of this study indicated that ndufv2 knockdown influences the actin cytoskeleton and tubulin stabilization in the leading process of migrating cortical neurons
The authors use green fluorescent-tagged complex I subunits to determine the kinetics of assembly. Tagged human NDUFV2 can replace the endogenous protein in the holo-complex I protein and was also found in complex I subassemblies suggesting that NDUFV2 may directly incorporate into the holo-enzyme and may also assemble into smaller complexes or in a monomer pool.
The authors provide an overview of the function and assembly of human complex I also known as NADH dehydrogenase (ubiquinone) 1 alpha subcomplex. They discuss its role in oxidative phosphorylation and the implications of complex I deficiency in disease.
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