Q9D5V5 · CUL5_MOUSE
- ProteinCullin-5
- GeneCul5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids780 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Core component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes), which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:17974915, PubMed:23806657, PubMed:24210661).
Acts a scaffold protein that contributes to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme (By similarity).
The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable SOCS box-containing substrate recognition component (By similarity).
Acts as a key regulator of neuron positioning during cortex development: component of various SOCS-containing ECS complexes, such as the ECS(SOCS7) complex, that regulate reelin signaling by mediating ubiquitination and degradation of DAB1 (PubMed:17974915, PubMed:24210661).
ECS(SOCS1) seems to direct ubiquitination of JAK2 (By similarity).
The ECS(SOCS2) complex mediates the ubiquitination and subsequent proteasomal degradation of phosphorylated EPOR and GHR (By similarity).
The ECS(SPSB3) complex catalyzes ubiquitination of nuclear CGAS (By similarity).
ECS(KLHDC1) complex is part of the DesCEND (destruction via C-end degrons) pathway and mediates ubiquitination and degradation of truncated SELENOS selenoprotein produced by failed UGA/Sec decoding, which ends with a glycine (By similarity).
The ECS(ASB9) complex mediates ubiquitination and degradation of CKB (By similarity).
As part of some ECS complex, promotes 'Lys-11'-linked ubiquitination and degradation of BTRC (By similarity).
As part of a multisubunit ECS complex, polyubiquitinates monoubiquitinated POLR2A. May form a cell surface vasopressin receptor (By similarity).
Acts a scaffold protein that contributes to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme (By similarity).
The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable SOCS box-containing substrate recognition component (By similarity).
Acts as a key regulator of neuron positioning during cortex development: component of various SOCS-containing ECS complexes, such as the ECS(SOCS7) complex, that regulate reelin signaling by mediating ubiquitination and degradation of DAB1 (PubMed:17974915, PubMed:24210661).
ECS(SOCS1) seems to direct ubiquitination of JAK2 (By similarity).
The ECS(SOCS2) complex mediates the ubiquitination and subsequent proteasomal degradation of phosphorylated EPOR and GHR (By similarity).
The ECS(SPSB3) complex catalyzes ubiquitination of nuclear CGAS (By similarity).
ECS(KLHDC1) complex is part of the DesCEND (destruction via C-end degrons) pathway and mediates ubiquitination and degradation of truncated SELENOS selenoprotein produced by failed UGA/Sec decoding, which ends with a glycine (By similarity).
The ECS(ASB9) complex mediates ubiquitination and degradation of CKB (By similarity).
As part of some ECS complex, promotes 'Lys-11'-linked ubiquitination and degradation of BTRC (By similarity).
As part of a multisubunit ECS complex, polyubiquitinates monoubiquitinated POLR2A. May form a cell surface vasopressin receptor (By similarity).
Pathway
Protein modification; protein ubiquitination.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Cul5-RING ubiquitin ligase complex | |
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Cellular Component | SCF ubiquitin ligase complex | |
Cellular Component | site of DNA damage | |
Molecular Function | protein-macromolecule adaptor activity | |
Molecular Function | ubiquitin ligase complex scaffold activity | |
Molecular Function | ubiquitin protein ligase binding | |
Biological Process | cerebral cortex radially oriented cell migration | |
Biological Process | proteasome-mediated ubiquitin-dependent protein catabolic process | |
Biological Process | protein ubiquitination | |
Biological Process | radial glia guided migration of Purkinje cell | |
Biological Process | reelin-mediated signaling pathway | |
Biological Process | regulation of cytosolic calcium ion concentration | |
Biological Process | regulation of neuron migration | |
Biological Process | SCF-dependent proteasomal ubiquitin-dependent protein catabolic process |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCullin-5
- Short namesCUL-5
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9D5V5
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes to sites of DNA damage in a UBAP2 and UBAP2L-dependent manner.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000119798 | 1-780 | Cullin-5 | |||
Sequence: MATSNLLKNKGSLQFEDKWDFMHPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGSSKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEVTLLGKQSSNKKSNMEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYDPQVNSPKDFTEGTLFSVNQDFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQMEWLIEHRYIRRDEADINTFIYMA | ||||||
Modified residue | 34 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 210 | Phosphothreonine | ||||
Sequence: T | ||||||
Cross-link | 724 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8) | ||||
Sequence: K |
Post-translational modification
Neddylated; which enhances the ubiquitination activity of ECS complexes and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN).
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes) formed of CUL5, Elongin BC (ELOB and ELOC), RNF7/RBX2 and a variable SOCS box domain-containing protein as substrate-specific recognition component (PubMed:17974915, PubMed:23806657, PubMed:24210661).
CUL5-containing ECS complexes specifically contain RNF7/RBX2, and not RBX1, as catalytic subunit (By similarity).
Component of the ECS(ASB2) complex with the substrate recognition component ASB2 (By similarity).
Component of the ECS(ASB6) complex with the substrate recognition component ASB6 (By similarity).
Component of the ECS(ASB7) complex with the substrate recognition component ASB7 (By similarity).
Component of the ECS(ASB9) complex with the substrate recognition component ASB9 (By similarity).
Component of the ECS(ASB11) complex with the substrate recognition component ASB11 (By similarity).
Component of the ECS(ASB12) complex with the substrate recognition component ASB12 (By similarity).
Component of the ECS(LRRC41) complex with the substrate recognition component LRRC41 (By similarity).
Component of the ECS(SOCS1) complex with the substrate recognition component SOCS1 (By similarity).
Component of the ECS(SOCS2) complex with the substrate recognition component SOCS2 (By similarity).
Component of the ECS(WSB1) complex with the substrate recognition subunit WSB1 (By similarity).
Component of the ECS(SOCS3) complex with the substrate recognition component SOCS3 (By similarity).
Component of the ECS(SOCS7) complex with the substrate recognition component SOCS7 (PubMed:24210661).
Component of the ECS(SPSB1) complex with the substrate recognition component SPSB1 (By similarity).
Component of the ECS(SPSB3) complex with the substrate recognition component SPSB3 (By similarity).
Component of the ECS(SPSB2) complex with the substrate recognition component SPSB2 (By similarity).
Component of the ECS(SPSB4) complex with the substrate recognition component SPSB4 (By similarity).
Component of the ECS(RAB40C) complex with the substrate recognition subunit RAB40C (By similarity).
Component of the ECS(KLHDC1) complex with the substrate recognition component KLHDC1 (By similarity).
Component of the ECS(PCMTD1) complex with the substrate recognition subunit PCMTD1 (By similarity).
May also form complexes containing RBX1 and ELOA or VHL; additional evidence is however required to confirm this result in vivo (By similarity).
Interacts (when neddylated) with ARIH2; leading to activate the E3 ligase activity of ARIH2 (By similarity).
Interacts with ERCC6; the interaction is induced by DNA damaging agents or inhibitors of RNA polymerase II elongation (By similarity).
Interacts with ELOA (via the BC-box) (By similarity).
Interacts (unneddylated form) with DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5; these interactions promote the cullin neddylation (By similarity).
CUL5-containing ECS complexes specifically contain RNF7/RBX2, and not RBX1, as catalytic subunit (By similarity).
Component of the ECS(ASB2) complex with the substrate recognition component ASB2 (By similarity).
Component of the ECS(ASB6) complex with the substrate recognition component ASB6 (By similarity).
Component of the ECS(ASB7) complex with the substrate recognition component ASB7 (By similarity).
Component of the ECS(ASB9) complex with the substrate recognition component ASB9 (By similarity).
Component of the ECS(ASB11) complex with the substrate recognition component ASB11 (By similarity).
Component of the ECS(ASB12) complex with the substrate recognition component ASB12 (By similarity).
Component of the ECS(LRRC41) complex with the substrate recognition component LRRC41 (By similarity).
Component of the ECS(SOCS1) complex with the substrate recognition component SOCS1 (By similarity).
Component of the ECS(SOCS2) complex with the substrate recognition component SOCS2 (By similarity).
Component of the ECS(WSB1) complex with the substrate recognition subunit WSB1 (By similarity).
Component of the ECS(SOCS3) complex with the substrate recognition component SOCS3 (By similarity).
Component of the ECS(SOCS7) complex with the substrate recognition component SOCS7 (PubMed:24210661).
Component of the ECS(SPSB1) complex with the substrate recognition component SPSB1 (By similarity).
Component of the ECS(SPSB3) complex with the substrate recognition component SPSB3 (By similarity).
Component of the ECS(SPSB2) complex with the substrate recognition component SPSB2 (By similarity).
Component of the ECS(SPSB4) complex with the substrate recognition component SPSB4 (By similarity).
Component of the ECS(RAB40C) complex with the substrate recognition subunit RAB40C (By similarity).
Component of the ECS(KLHDC1) complex with the substrate recognition component KLHDC1 (By similarity).
Component of the ECS(PCMTD1) complex with the substrate recognition subunit PCMTD1 (By similarity).
May also form complexes containing RBX1 and ELOA or VHL; additional evidence is however required to confirm this result in vivo (By similarity).
Interacts (when neddylated) with ARIH2; leading to activate the E3 ligase activity of ARIH2 (By similarity).
Interacts with ERCC6; the interaction is induced by DNA damaging agents or inhibitors of RNA polymerase II elongation (By similarity).
Interacts with ELOA (via the BC-box) (By similarity).
Interacts (unneddylated form) with DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5; these interactions promote the cullin neddylation (By similarity).
Protein-protein interaction databases
Miscellaneous
Structure
Sequence
- Sequence statusComplete
- Length780
- Mass (Da)90,974
- Last updated2007-01-23 v3
- ChecksumC6EE7A1AF1038C0D
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 573 | in Ref. 1; BAC27621 | ||||
Sequence: W → C |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK014894 EMBL· GenBank· DDBJ | BAB29609.1 EMBL· GenBank· DDBJ | mRNA | ||
AK030306 EMBL· GenBank· DDBJ | BAC26889.1 EMBL· GenBank· DDBJ | mRNA | ||
AK031955 EMBL· GenBank· DDBJ | BAC27621.1 EMBL· GenBank· DDBJ | mRNA | ||
AK046030 EMBL· GenBank· DDBJ | BAC32575.1 EMBL· GenBank· DDBJ | mRNA | ||
AK080305 EMBL· GenBank· DDBJ | BAC37872.1 EMBL· GenBank· DDBJ | mRNA | ||
BC075710 EMBL· GenBank· DDBJ | AAH75710.1 EMBL· GenBank· DDBJ | mRNA |