Q9CUB6 · OTUD1_MOUSE
- ProteinOTU domain-containing protein 1
- GeneOtud1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids454 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin. Required for the stability and translation of a subset mRNAs with a high abundance of rare codons by mediating deubiquitination of 40S ribosomal protein RPS10/eS10, thereby antagonizing ZNF598-mediated 40S ubiquitination. The abundance of rare codons in mRNAs can limit the translation rate and can lead to ribosome collisions that trigger activation of ribosome quality control (RQC) pathway by ZNF598. OTUD1-mediated deubiquitination prevents activation of the RQC and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation.
Catalytic activity
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 290 | |||||
Sequence: D | ||||||
Active site | 293 | Nucleophile | ||||
Sequence: C | ||||||
Active site | 404 | |||||
Sequence: H |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | cysteine-type deubiquitinase activity | |
Biological Process | protein K63-linked deubiquitination | |
Biological Process | proteolysis |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameOTU domain-containing protein 1
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9CUB6
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000271019 | 1-454 | OTU domain-containing protein 1 | |||
Sequence: MQLYSSVCTHYPAGTPGPTAAAPPATAAAAFKVSLQSASPAAAAPEPDTGERPPAAATEPREAAAAAAMPAFSACFERSGSAAAPPGACSKPPLPPHFTSTAHIAVRALGAERLLLPPPSAPSPPRRGSSAWLLEELLRPDEPAAPNAVRDAPDRNFRLSEHRQALAASQHRAPAPAPVGPEPGAGPGSGPWGEERRAERSSRGWDRASGRSDASGSDALRRQDPEAEAHPVPAPARSSGEPAQNGEGEAVGTSRADPRDEKLALYLAEVERQDKYLRQRNKYRFHIIPDGNCLYRAVSKTVYGDQSLHRELREQTVHYIADHLDHFSPLIEGDVGEFIIAAAQDGAWAGYPELLAMGQMLNVNIHLTTGGRLESPTVSTMIHYLGPEDSLRPSIWLSWLSNGHYDAVFDHSYPNPEYDNWCKQTQIQKKRDEELAKSMAISLSKMYIEQNACS |
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 36-64 | Disordered | ||||
Sequence: QSASPAAAAPEPDTGERPPAAATEPREAA | ||||||
Region | 116-257 | Disordered | ||||
Sequence: LPPPSAPSPPRRGSSAWLLEELLRPDEPAAPNAVRDAPDRNFRLSEHRQALAASQHRAPAPAPVGPEPGAGPGSGPWGEERRAERSSRGWDRASGRSDASGSDALRRQDPEAEAHPVPAPARSSGEPAQNGEGEAVGTSRAD | ||||||
Compositional bias | 193-207 | Basic and acidic residues | ||||
Sequence: GEERRAERSSRGWDR | ||||||
Domain | 282-411 | OTU | ||||
Sequence: KYRFHIIPDGNCLYRAVSKTVYGDQSLHRELREQTVHYIADHLDHFSPLIEGDVGEFIIAAAQDGAWAGYPELLAMGQMLNVNIHLTTGGRLESPTVSTMIHYLGPEDSLRPSIWLSWLSNGHYDAVFDH | ||||||
Region | 287-293 | Cys-loop | ||||
Sequence: IIPDGNC | ||||||
Region | 342-352 | His-loop | ||||
Sequence: AAQDGAWAGYP | ||||||
Region | 399-404 | Variable-loop | ||||
Sequence: WLSNGH | ||||||
Domain | 430-449 | UIM | ||||
Sequence: KRDEELAKSMAISLSKMYIE |
Domain
The UIM repeat increases the specificity and efficiency of the enzyme toward 'Lys-63'-linked polyubiquitin.
Specificity is not given by the S1' ubiquitin-binding site within the OTU domain (composed of the Cys-, His- and Variable-loops).
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length454
- Mass (Da)48,823
- Last updated2007-01-09 v2
- ChecksumBAF1702C35D1EF89
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 193-207 | Basic and acidic residues | ||||
Sequence: GEERRAERSSRGWDR |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL928877 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AK016972 EMBL· GenBank· DDBJ | BAB30530.1 EMBL· GenBank· DDBJ | mRNA | Frameshift |