Q9CQD1 · RAB5A_MOUSE
- ProteinRas-related protein Rab-5A
- GeneRab5a
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids215 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (By similarity).
Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (PubMed:18425118).
Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (PubMed:18425118).
Catalytic activity
- GTP + H2O = GDP + H+ + phosphateThis reaction proceeds in the forward direction.
Activity regulation
Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 27-35 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GESAVGKSS | ||||||
Binding site | 46-52 | GTP (UniProtKB | ChEBI) | ||||
Sequence: HEFQEST | ||||||
Binding site | 75-79 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DTAGQ | ||||||
Binding site | 133-136 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NKAD | ||||||
Binding site | 163-165 | GTP (UniProtKB | ChEBI) | ||||
Sequence: SAK |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRas-related protein Rab-5A
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9CQD1
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Lipid-anchor
Early endosome membrane ; Lipid-anchor
Note: Enriched in stage I melanosomes. Alternates between membrane-bound and cytosolic forms.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 34 | Inhibits endosome localization. Disrupts interaction of RAB5A and OCRL with INPP5F. Prevents its localization to apoptotic cell corpse-containing early phagosomes. Maturation of apoptotic cell-containing phagosome into acidic phagosomes is decreased. Interacts with PIK3C3. | ||||
Sequence: S → N | ||||||
Mutagenesis | 79 | Induces the formation of larger endosomes. No effect on interaction with INPP5F. Prevents interaction with DYN2 without affecting the interaction with PIK3C3. | ||||
Sequence: Q → L |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 10 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue, lipidation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000121106 | 1-215 | Ras-related protein Rab-5A | |||
Sequence: MANRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPAQPARSQCCSN | ||||||
Modified residue | 84 | Phosphoserine | ||||
Sequence: S | ||||||
Lipidation | 212 | S-geranylgeranyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 213 | S-geranylgeranyl cysteine | ||||
Sequence: C |
Post-translational modification
Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including RAB GDP dissociation inhibitors GDI1 and GDI2.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Interacts with GDI1; this promotes dissociation from membranes; phosphorylation at Ser-84 disrupts this interaction (By similarity).
Interacts with GDI2; phosphorylation at Ser-84 disrupts the interaction (By similarity).
Interacts with EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with RINL. Interacts with ALS2CL, SUN2, ZFYVE20 and RUFY1. Interacts with RABEP1; one RABEP1 homodimer binds two RAB5A chains, but at opposite sides of the dimer (By similarity).
Interacts with SGSM1 and SGSM3 (PubMed:17509819).
Interacts with PIK3CB (PubMed:21059846).
Interacts with OCRL and INPP5F (PubMed:25869668).
May be a component of a complex composed of RAB5A, DYN2 and PIK3C3 (PubMed:18425118).
Does not interact with BLOC-3 complex (heterodimer of HPS1 and HPS4). Interacts with CLN5 (By similarity).
Interacts with APPL2 (PubMed:25568335).
Interacts with F8A1/F8A2/F8A3 (By similarity).
Found in a complex with F8A1/F8A2/F8A3, HTT and RAB5A; mediates the recruitment of HTT by RAB5A onto early endosomes (By similarity).
Interacts with ATP9A (By similarity).
Interacts with PPP1R21; mediates the recruitment of FERRY complex by RAB5A onto early endosomes (By similarity).
Interacts with GDI2; phosphorylation at Ser-84 disrupts the interaction (By similarity).
Interacts with EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with RINL. Interacts with ALS2CL, SUN2, ZFYVE20 and RUFY1. Interacts with RABEP1; one RABEP1 homodimer binds two RAB5A chains, but at opposite sides of the dimer (By similarity).
Interacts with SGSM1 and SGSM3 (PubMed:17509819).
Interacts with PIK3CB (PubMed:21059846).
Interacts with OCRL and INPP5F (PubMed:25869668).
May be a component of a complex composed of RAB5A, DYN2 and PIK3C3 (PubMed:18425118).
Does not interact with BLOC-3 complex (heterodimer of HPS1 and HPS4). Interacts with CLN5 (By similarity).
Interacts with APPL2 (PubMed:25568335).
Interacts with F8A1/F8A2/F8A3 (By similarity).
Found in a complex with F8A1/F8A2/F8A3, HTT and RAB5A; mediates the recruitment of HTT by RAB5A onto early endosomes (By similarity).
Interacts with ATP9A (By similarity).
Interacts with PPP1R21; mediates the recruitment of FERRY complex by RAB5A onto early endosomes (By similarity).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for motif, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 49-57 | Effector region | ||||
Sequence: QESTIGAAF | ||||||
Region | 181-215 | Disordered | ||||
Sequence: LPKNEPQNPGANSARGRGVDLTEPAQPARSQCCSN |
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length215
- Mass (Da)23,599
- Last updated2001-06-01 v1
- Checksum9CDE2FD9B37C261A
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 13 | in Ref. 3; BAE29485 | ||||
Sequence: N → D | ||||||
Sequence conflict | 119 | in Ref. 3; BAB22245 | ||||
Sequence: Q → R |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB232593 EMBL· GenBank· DDBJ | BAF02855.1 EMBL· GenBank· DDBJ | mRNA | ||
AK002631 EMBL· GenBank· DDBJ | BAB22245.1 EMBL· GenBank· DDBJ | mRNA | ||
AK008198 EMBL· GenBank· DDBJ | BAB25527.1 EMBL· GenBank· DDBJ | mRNA | ||
AK010495 EMBL· GenBank· DDBJ | BAB26985.1 EMBL· GenBank· DDBJ | mRNA | ||
AK081999 EMBL· GenBank· DDBJ | BAC38391.1 EMBL· GenBank· DDBJ | mRNA | ||
AK150296 EMBL· GenBank· DDBJ | BAE29448.1 EMBL· GenBank· DDBJ | mRNA | ||
AK150346 EMBL· GenBank· DDBJ | BAE29485.1 EMBL· GenBank· DDBJ | mRNA | ||
AK150405 EMBL· GenBank· DDBJ | BAE29531.1 EMBL· GenBank· DDBJ | mRNA | ||
AK151066 EMBL· GenBank· DDBJ | BAE30082.1 EMBL· GenBank· DDBJ | mRNA | ||
AK153131 EMBL· GenBank· DDBJ | BAE31744.1 EMBL· GenBank· DDBJ | mRNA | ||
AK159706 EMBL· GenBank· DDBJ | BAE35305.1 EMBL· GenBank· DDBJ | mRNA | ||
CH466559 EMBL· GenBank· DDBJ | EDL23663.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC004842 EMBL· GenBank· DDBJ | AAH04842.1 EMBL· GenBank· DDBJ | mRNA | ||
BC034370 EMBL· GenBank· DDBJ | AAH34370.1 EMBL· GenBank· DDBJ | mRNA | ||
BC096481 EMBL· GenBank· DDBJ | AAH96481.1 EMBL· GenBank· DDBJ | mRNA |