Q9CA86 · PEX2_ARATH

Function

function

E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling (PubMed:17478547, PubMed:20679226, PubMed:23336935, PubMed:25214533).
The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane (By similarity).
PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase (PubMed:17478547, PubMed:20679226, PubMed:23336935).
PEX2 ubiquitinates PEX5 during its passage through the retrotranslocation channel: catalyzes monoubiquitination of PEX5 at 'Cys-6', a modification that acts as a signal for PEX5 extraction into the cytosol (By similarity).

Catalytic activity

  • [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine.
    EC:2.3.2.36 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site277Zn2+ 1 (UniProtKB | ChEBI)
Binding site280Zn2+ 1 (UniProtKB | ChEBI)
Binding site293Zn2+ 2 (UniProtKB | ChEBI)
Binding site295Zn2+ 2 (UniProtKB | ChEBI)
Binding site298Zn2+ 1 (UniProtKB | ChEBI)
Binding site301Zn2+ 1 (UniProtKB | ChEBI)
Binding site314Zn2+ 2 (UniProtKB | ChEBI)
Binding site317Zn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentperoxisomal membrane
Cellular Componentperoxisome
Cellular Componentplasmodesma
Molecular Functionubiquitin-protein transferase activity
Molecular Functionzinc ion binding
Biological Processfatty acid beta-oxidation
Biological Processperoxisome organization
Biological Processphotomorphogenesis
Biological Processprotein import into peroxisome matrix
Biological Processprotein monoubiquitination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Peroxisome biogenesis protein 2
  • EC number
  • Alternative names
    • E3 ubiquitin-protein ligase PEX2
    • Peroxin-2 (AtPEX2
      ; AthPEX2)
    • Pex2p

Gene names

    • Name
      PEX2
    • Synonyms
      TED3
    • ORF names
      F19K16.23, F20B17.23
    • Ordered locus names
      At1g79810

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9CA86
  • Secondary accessions
    • Q8LFI7
    • Q9M9Z9

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-38Peroxisomal matrix
Transmembrane39-65Helical; Name=TM1
Topological domain66-71Cytoplasmic
Transmembrane72-97Helical; Name=TM2
Topological domain98-131Peroxisomal matrix
Transmembrane132-158Helical; Name=TM3
Topological domain159-168Cytoplasmic
Transmembrane169-199Helical; Name=TM4
Topological domain200-226Peroxisomal matrix
Transmembrane227-250Helical; Name=TM5
Topological domain251-333Cytoplasmic

Keywords

Phenotypes & Variants

Disruption phenotype

Embryo lethality at the heart stage.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis55In pex2-2 mutant; defects peroxisomal matrix protein degradation.
Mutagenesis161In pex2-1 mutant; defects peroxisomal matrix protein degradation.
Mutagenesis275In ted3; suppressor of the det1 mutant phenotype.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 15 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000563751-333Peroxisome biogenesis protein 2

Proteomic databases

Expression

Tissue specificity

Expressed in roots, stems, leaves, flowers, pollen, ovules, seeds and siliques.

Developmental stage

Expressed throughout development.

Gene expression databases

Interaction

Subunit

Component of the PEX2-PEX10-PEX12 retrotranslocation channel (By similarity).
Interacts with DSK2a and DSK2b (PubMed:23336935).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q9CA86DSK2A Q9SII93EBI-4470254, EBI-6860633
BINARY Q9CA86DSK2B Q9SII84EBI-4470254, EBI-4433040

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for zinc finger.

TypeIDPosition(s)Description
Zinc finger277-318RING-type

Domain

The three subunits of the retrotranslocation channel (PEX2, PEX10 and PEX12) coassemble in the membrane into a channel with an open 10 Angstrom pore. The RING-type zinc-fingers that catalyze PEX5 receptor ubiquitination are positioned above the pore on the cytosolic side of the complex.

Sequence similarities

Belongs to the pex2/pex10/pex12 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete

This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.

Q9CA86-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    333
  • Mass (Da)
    38,174
  • Last updated
    2001-06-01 v1
  • Checksum
    5A1511BA37B63A19
MTPSTPADDAWIRSYQRLLPESQSLLASRRSVIPVAISRVNQFDAARLDVEMSAMLKEQLVKVFTLMKPGMLFQYEPELDAFLEFLIWRFSIWVDKPTPGNALMNLRYRDERGVVAQHLGKVRTGLEGPGLTSPQKIWYCVASVGGQYLFSRLQSFSAFRRWGDSEQRPLARRLWTLVQRIEGIYKAASFLNLLSFLYTGRYRNLIEKALKARLVYRSPHMNRSVSFEYMNRQLVWNEFSEMLLLLLPLLNSSAVKNILSPFAKDKSSSTKEDTVTCPICQVDPAIPFIALPCQHRYCYYCIRTRCASAASFRCLRCNEPVVAIQREGVSSGK

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
F4HQB1F4HQB1_ARATHTED3282

Sequence caution

The sequence AAF68113.1 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC010793
EMBL· GenBank· DDBJ
AAF68113.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AC011717
EMBL· GenBank· DDBJ
AAG52254.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE36301.1
EMBL· GenBank· DDBJ
Genomic DNA
AY064063
EMBL· GenBank· DDBJ
AAL36419.1
EMBL· GenBank· DDBJ
mRNA
AY096373
EMBL· GenBank· DDBJ
AAM20014.1
EMBL· GenBank· DDBJ
mRNA
AY084823
EMBL· GenBank· DDBJ
AAM61388.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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