Q9CA86 · PEX2_ARATH
- ProteinPeroxisome biogenesis protein 2
- GenePEX2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids333 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling (PubMed:17478547, PubMed:20679226, PubMed:23336935, PubMed:25214533).
The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane (By similarity).
PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase (PubMed:17478547, PubMed:20679226, PubMed:23336935).
PEX2 ubiquitinates PEX5 during its passage through the retrotranslocation channel: catalyzes monoubiquitination of PEX5 at 'Cys-6', a modification that acts as a signal for PEX5 extraction into the cytosol (By similarity).
The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane (By similarity).
PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase (PubMed:17478547, PubMed:20679226, PubMed:23336935).
PEX2 ubiquitinates PEX5 during its passage through the retrotranslocation channel: catalyzes monoubiquitination of PEX5 at 'Cys-6', a modification that acts as a signal for PEX5 extraction into the cytosol (By similarity).
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 277 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 280 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 293 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 295 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 298 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 301 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 314 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 317 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | peroxisomal membrane | |
Cellular Component | peroxisome | |
Cellular Component | plasmodesma | |
Molecular Function | ubiquitin-protein transferase activity | |
Molecular Function | zinc ion binding | |
Biological Process | fatty acid beta-oxidation | |
Biological Process | peroxisome organization | |
Biological Process | photomorphogenesis | |
Biological Process | protein import into peroxisome matrix | |
Biological Process | protein monoubiquitination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePeroxisome biogenesis protein 2
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9CA86
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Peroxisome membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-38 | Peroxisomal matrix | ||||
Sequence: MTPSTPADDAWIRSYQRLLPESQSLLASRRSVIPVAIS | ||||||
Transmembrane | 39-65 | Helical; Name=TM1 | ||||
Sequence: RVNQFDAARLDVEMSAMLKEQLVKVFT | ||||||
Topological domain | 66-71 | Cytoplasmic | ||||
Sequence: LMKPGM | ||||||
Transmembrane | 72-97 | Helical; Name=TM2 | ||||
Sequence: LFQYEPELDAFLEFLIWRFSIWVDKP | ||||||
Topological domain | 98-131 | Peroxisomal matrix | ||||
Sequence: TPGNALMNLRYRDERGVVAQHLGKVRTGLEGPGL | ||||||
Transmembrane | 132-158 | Helical; Name=TM3 | ||||
Sequence: TSPQKIWYCVASVGGQYLFSRLQSFSA | ||||||
Topological domain | 159-168 | Cytoplasmic | ||||
Sequence: FRRWGDSEQR | ||||||
Transmembrane | 169-199 | Helical; Name=TM4 | ||||
Sequence: PLARRLWTLVQRIEGIYKAASFLNLLSFLYT | ||||||
Topological domain | 200-226 | Peroxisomal matrix | ||||
Sequence: GRYRNLIEKALKARLVYRSPHMNRSVS | ||||||
Transmembrane | 227-250 | Helical; Name=TM5 | ||||
Sequence: FEYMNRQLVWNEFSEMLLLLLPLL | ||||||
Topological domain | 251-333 | Cytoplasmic | ||||
Sequence: NSSAVKNILSPFAKDKSSSTKEDTVTCPICQVDPAIPFIALPCQHRYCYYCIRTRCASAASFRCLRCNEPVVAIQREGVSSGK |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Embryo lethality at the heart stage.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 55 | In pex2-2 mutant; defects peroxisomal matrix protein degradation. | ||||
Sequence: M → I | ||||||
Mutagenesis | 161 | In pex2-1 mutant; defects peroxisomal matrix protein degradation. | ||||
Sequence: R → K | ||||||
Mutagenesis | 275 | In ted3; suppressor of the det1 mutant phenotype. | ||||
Sequence: V → M |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 15 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000056375 | 1-333 | Peroxisome biogenesis protein 2 | |||
Sequence: MTPSTPADDAWIRSYQRLLPESQSLLASRRSVIPVAISRVNQFDAARLDVEMSAMLKEQLVKVFTLMKPGMLFQYEPELDAFLEFLIWRFSIWVDKPTPGNALMNLRYRDERGVVAQHLGKVRTGLEGPGLTSPQKIWYCVASVGGQYLFSRLQSFSAFRRWGDSEQRPLARRLWTLVQRIEGIYKAASFLNLLSFLYTGRYRNLIEKALKARLVYRSPHMNRSVSFEYMNRQLVWNEFSEMLLLLLPLLNSSAVKNILSPFAKDKSSSTKEDTVTCPICQVDPAIPFIALPCQHRYCYYCIRTRCASAASFRCLRCNEPVVAIQREGVSSGK |
Proteomic databases
Expression
Tissue specificity
Expressed in roots, stems, leaves, flowers, pollen, ovules, seeds and siliques.
Developmental stage
Expressed throughout development.
Gene expression databases
Interaction
Subunit
Component of the PEX2-PEX10-PEX12 retrotranslocation channel (By similarity).
Interacts with DSK2a and DSK2b (PubMed:23336935).
Interacts with DSK2a and DSK2b (PubMed:23336935).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9CA86 | DSK2A Q9SII9 | 3 | EBI-4470254, EBI-6860633 | |
BINARY | Q9CA86 | DSK2B Q9SII8 | 4 | EBI-4470254, EBI-4433040 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 277-318 | RING-type | ||||
Sequence: CPICQVDPAIPFIALPCQHRYCYYCIRTRCASAASFRCLRCN |
Domain
The three subunits of the retrotranslocation channel (PEX2, PEX10 and PEX12) coassemble in the membrane into a channel with an open 10 Angstrom pore. The RING-type zinc-fingers that catalyze PEX5 receptor ubiquitination are positioned above the pore on the cytosolic side of the complex.
Sequence similarities
Belongs to the pex2/pex10/pex12 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q9CA86-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length333
- Mass (Da)38,174
- Last updated2001-06-01 v1
- Checksum5A1511BA37B63A19
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4HQB1 | F4HQB1_ARATH | TED3 | 282 |
Sequence caution
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC010793 EMBL· GenBank· DDBJ | AAF68113.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AC011717 EMBL· GenBank· DDBJ | AAG52254.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE36301.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY064063 EMBL· GenBank· DDBJ | AAL36419.1 EMBL· GenBank· DDBJ | mRNA | ||
AY096373 EMBL· GenBank· DDBJ | AAM20014.1 EMBL· GenBank· DDBJ | mRNA | ||
AY084823 EMBL· GenBank· DDBJ | AAM61388.1 EMBL· GenBank· DDBJ | mRNA |