Q9CA83 · MAOP4_ARATH
- ProteinNADP-dependent malic enzyme 4, chloroplastic
- GeneNADP-ME4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids646 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO2 released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO2 that occurs in most plants (By similarity).
Catalytic activity
- (S)-malate + NADP+ = pyruvate + CO2 + NADPH
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Divalent metal cations. Prefers magnesium or manganese.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
10.2 μM | NADP | 7.5 | ||||
0.23 mM | malate | 7.5 |
kcat is 151.3 sec-1 with NADP as substrate (at pH 7.5).
Pathway
Photosynthesis; C3 acid pathway.
Features
Showing features for active site, binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 194 | Proton donor | |||
Binding site | 247 | NADP+ (UniProtKB | ChEBI) | |||
Active site | 265 | Proton acceptor | |||
Binding site | 337 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 338 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 361 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 361 | NADP+ (UniProtKB | ChEBI) | |||
Site | 361 | Important for activity | |||
Binding site | 390-406 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 502 | NADP+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | mitochondrion | |
Molecular Function | cobalt ion binding | |
Molecular Function | malate dehydrogenase (decarboxylating) (NADP+) activity | |
Molecular Function | NAD binding | |
Molecular Function | oxaloacetate decarboxylase activity | |
Molecular Function | zinc ion binding | |
Biological Process | fatty acid biosynthetic process | |
Biological Process | malate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADP-dependent malic enzyme 4, chloroplastic
- EC number
- Short namesAtNADP-ME4; NADP-malic enzyme 4
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9CA83
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transit peptide | 1-74 | Chloroplast | |||
Chain | PRO_0000420152 | 75-646 | NADP-dependent malic enzyme 4, chloroplastic | ||
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in leaves, stems, flowers and roots, mainly in vascular system. In roots, present in the stele, including the vascular tissue and the pericycle, mainly at emerging lateral roots and at root tips.
Developmental stage
During embryogenesis, present in the endosperm and the embryo at all developmental stages, including the seed attachment point and the integuments. Also detected in siliques. During germination, first confined to the radicle to later approaches the meristematic region. Finally present in the whole root. Expressed in cotyledons and primary leaves. In opened flowers, present in sepals, stigma, filaments, and pollen.
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length646
- Mass (Da)71,161
- Last updated2001-06-01 v1
- Checksum715FB567827EA71A
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC010793 EMBL· GenBank· DDBJ | AAF68116.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AC011717 EMBL· GenBank· DDBJ | AAG52235.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE36294.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY060584 EMBL· GenBank· DDBJ | AAL31209.1 EMBL· GenBank· DDBJ | mRNA | ||
AY142064 EMBL· GenBank· DDBJ | AAM98328.1 EMBL· GenBank· DDBJ | mRNA | ||
AK222053 EMBL· GenBank· DDBJ | BAD94826.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |