Q9CA83 · MAOP4_ARATH

Function

function

The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO2 released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO2 that occurs in most plants (By similarity).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Divalent metal cations. Prefers magnesium or manganese.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
10.2 μMNADP7.5
0.23 mMmalate7.5
kcat is 151.3 sec-1 with NADP as substrate (at pH 7.5).

Pathway

Photosynthesis; C3 acid pathway.

Features

Showing features for active site, binding site, site.

Type
IDPosition(s)Description
Active site194Proton donor
Binding site247NADP+ (UniProtKB | ChEBI)
Active site265Proton acceptor
Binding site337a divalent metal cation (UniProtKB | ChEBI)
Binding site338a divalent metal cation (UniProtKB | ChEBI)
Binding site361a divalent metal cation (UniProtKB | ChEBI)
Binding site361NADP+ (UniProtKB | ChEBI)
Site361Important for activity
Binding site390-406NADP+ (UniProtKB | ChEBI)
Binding site502NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentmitochondrion
Molecular Functioncobalt ion binding
Molecular Functionmalate dehydrogenase (decarboxylating) (NADP+) activity
Molecular FunctionNAD binding
Molecular Functionoxaloacetate decarboxylase activity
Molecular Functionzinc ion binding
Biological Processfatty acid biosynthetic process
Biological Processmalate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADP-dependent malic enzyme 4, chloroplastic
  • EC number
  • Short names
    AtNADP-ME4; NADP-malic enzyme 4

Gene names

    • Name
      NADP-ME4
    • ORF names
      F19K16.27
    • Ordered locus names
      At1g79750

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9CA83
  • Secondary accessions
    • Q56WI7
    • Q9MA03

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

Type
IDPosition(s)Description
Transit peptide1-74Chloroplast
ChainPRO_000042015275-646NADP-dependent malic enzyme 4, chloroplastic

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in leaves, stems, flowers and roots, mainly in vascular system. In roots, present in the stele, including the vascular tissue and the pericycle, mainly at emerging lateral roots and at root tips.

Developmental stage

During embryogenesis, present in the endosperm and the embryo at all developmental stages, including the seed attachment point and the integuments. Also detected in siliques. During germination, first confined to the radicle to later approaches the meristematic region. Finally present in the whole root. Expressed in cotyledons and primary leaves. In opened flowers, present in sepals, stigma, filaments, and pollen.

Gene expression databases

Interaction

Subunit

Homodimer and homotetramer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the malic enzymes family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    646
  • Mass (Da)
    71,161
  • Last updated
    2001-06-01 v1
  • Checksum
    715FB567827EA71A
MISLTPSLFLNKTVVPGCSTRLSLRQPRTIITPPASLRVFSSLGSNQDPTGSVLIETTATSSSSLETSAADIVPKSTVSGGVQDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEYSELMHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLIAALRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIVSSRKESIQHFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKLNEKPIILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKAYELGLATRLPQPKELEQCAESSMYSPSYRSYR

Sequence caution

The sequence AAF68116.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence BAD94826.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC010793
EMBL· GenBank· DDBJ
AAF68116.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AC011717
EMBL· GenBank· DDBJ
AAG52235.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE36294.1
EMBL· GenBank· DDBJ
Genomic DNA
AY060584
EMBL· GenBank· DDBJ
AAL31209.1
EMBL· GenBank· DDBJ
mRNA
AY142064
EMBL· GenBank· DDBJ
AAM98328.1
EMBL· GenBank· DDBJ
mRNA
AK222053
EMBL· GenBank· DDBJ
BAD94826.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

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