Q9C942 · CSE_ARATH
- ProteinCaffeoylshikimate esterase
- GeneCSE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids332 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Esterase involved in the biosynthesis of lignin. Hydrolyzes caffeoylshikimate into caffeate and shikimate. Together with 4-coumarate--CoA ligase (4CL), acts on an alternative reaction for the formation of caffeoyl-CoA and bypasses the second reaction of shikimate O-hydroxycinnamoyltransferase (HST). Accepts also 4-coumaroylshikimate as substrate, but with lower activity. According to PubMed:20345607 and PubMed:22915575, possesses monoacylglycerol O-acyltransferase, monoacylglycerol lipase and lysophospholipase activities in vitro. With the association of ACBP2, may promote the degradation of lysophosphatidylcholine and detoxify the peroxidized membrane in response to cadmium-induced oxidative stress. However these results require additional confirmation in vivo.
Miscellaneous
Mutant plants exhibit increased sensitivity to zinc, cadmium and H2O2.
Catalytic activity
- 5-O-[(E)-caffeoyl]-shikimate + H2O = shikimate + (E)-caffeate + H+
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
96.5 μM | caffeoylshikimate | |||||
211 μM | 4-coumarylshikimate | |||||
6.6 μM | lysophosphatidylcholine | 8.0 | 33 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
9.3 pmol/sec/mg | toward caffeoylshikimate | ||||
0.66 pmol/sec/mg | toward 4-coumarylshikimate | ||||
0.03 μmol/min/mg | 8.0 | 33 | toward lysophosphatidylcholine |
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 147 | Nucleophile | |||
Active site | 268 | Charge relay system | |||
Active site | 298 | Charge relay system | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | Golgi apparatus | |
Cellular Component | nucleus | |
Cellular Component | plasma membrane | |
Cellular Component | plasmodesma | |
Molecular Function | 2-acylglycerol O-acyltransferase activity | |
Molecular Function | caffeoyl-CoA: alcohol caffeoyl transferase activity | |
Molecular Function | hydrolase activity | |
Molecular Function | lysophospholipase activity | |
Biological Process | lignin biosynthetic process | |
Biological Process | response to cadmium ion | |
Biological Process | response to hydrogen peroxide | |
Biological Process | response to oxidative stress | |
Biological Process | response to zinc ion |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCaffeoylshikimate esterase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9C942
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Note: Colocalizes with ACBP2.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Reduced height and weight of senescent plants due to reduced lignin content.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 29 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000424295 | 1-332 | Caffeoylshikimate esterase | ||
Proteomic databases
Expression
Tissue specificity
Expressed in vasculature of roots and leaves, stems, flowers and siliques.
Induction
By zinc and H2O2.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-26 | Disordered | |||
Sequence similarities
Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length332
- Mass (Da)36,975
- Last updated2001-06-01 v1
- Checksum985297835CF58AE6
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC019018 EMBL· GenBank· DDBJ | AAG52273.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE32849.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY054577 EMBL· GenBank· DDBJ | AAK96768.1 EMBL· GenBank· DDBJ | mRNA | ||
BT008729 EMBL· GenBank· DDBJ | AAP42742.1 EMBL· GenBank· DDBJ | mRNA |