Q9C942 · CSE_ARATH

Function

function

Esterase involved in the biosynthesis of lignin. Hydrolyzes caffeoylshikimate into caffeate and shikimate. Together with 4-coumarate--CoA ligase (4CL), acts on an alternative reaction for the formation of caffeoyl-CoA and bypasses the second reaction of shikimate O-hydroxycinnamoyltransferase (HST). Accepts also 4-coumaroylshikimate as substrate, but with lower activity. According to PubMed:20345607 and PubMed:22915575, possesses monoacylglycerol O-acyltransferase, monoacylglycerol lipase and lysophospholipase activities in vitro. With the association of ACBP2, may promote the degradation of lysophosphatidylcholine and detoxify the peroxidized membrane in response to cadmium-induced oxidative stress. However these results require additional confirmation in vivo.

Miscellaneous

Mutant plants exhibit increased sensitivity to zinc, cadmium and H2O2.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
96.5 μMcaffeoylshikimate
211 μM4-coumarylshikimate
6.6 μMlysophosphatidylcholine8.033
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
9.3 pmol/sec/mgtoward caffeoylshikimate
0.66 pmol/sec/mgtoward 4-coumarylshikimate
0.03 μmol/min/mg8.033toward lysophosphatidylcholine

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site147Nucleophile
Active site268Charge relay system
Active site298Charge relay system

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular ComponentGolgi apparatus
Cellular Componentnucleus
Cellular Componentplasma membrane
Cellular Componentplasmodesma
Molecular Function2-acylglycerol O-acyltransferase activity
Molecular Functioncaffeoyl-CoA: alcohol caffeoyl transferase activity
Molecular Functionhydrolase activity
Molecular Functionlysophospholipase activity
Biological Processlignin biosynthetic process
Biological Processresponse to cadmium ion
Biological Processresponse to hydrogen peroxide
Biological Processresponse to oxidative stress
Biological Processresponse to zinc ion

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Caffeoylshikimate esterase
  • EC number
  • Alternative names
    • Lysophospholipase 2 (LysoPL2)

Gene names

    • Name
      CSE
    • ORF names
      F14G24.3
    • Ordered locus names
      At1g52760

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9C942

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Cell membrane
; Peripheral membrane protein
Note: Colocalizes with ACBP2.

Keywords

Phenotypes & Variants

Disruption phenotype

Reduced height and weight of senescent plants due to reduced lignin content.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 29 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004242951-332Caffeoylshikimate esterase

Proteomic databases

Expression

Tissue specificity

Expressed in vasculature of roots and leaves, stems, flowers and siliques.

Induction

By zinc and H2O2.

Gene expression databases

Interaction

Subunit

Interacts with ACBP2.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-26Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    332
  • Mass (Da)
    36,975
  • Last updated
    2001-06-01 v1
  • Checksum
    985297835CF58AE6
MPSEAESSANSAPATPPPPPNFWGTMPEEEYYTSQGVRNSKSYFETPNGKLFTQSFLPLDGEIKGTVYMSHGYGSDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHVRCSDPYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLFAYGLLFGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQRYTGKPRVGTMRELLRKTQYVQENFGKVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENAEIVLKDMREWIDEKVKKYGSKTA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC019018
EMBL· GenBank· DDBJ
AAG52273.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE32849.1
EMBL· GenBank· DDBJ
Genomic DNA
AY054577
EMBL· GenBank· DDBJ
AAK96768.1
EMBL· GenBank· DDBJ
mRNA
BT008729
EMBL· GenBank· DDBJ
AAP42742.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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