Q9C5R8 · BAS1B_ARATH

Function

function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. May be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf.

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin CDSP32.

Catalytic activity

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site126Cysteine sulfenic acid (-SOH) intermediate

GO annotations

AspectTerm
Cellular Componentapoplast
Cellular Componentchloroplast
Cellular Componentchloroplast stroma
Cellular Componentplasma membrane
Cellular Componentstromule
Molecular Functionperoxiredoxin activity
Molecular Functionthioredoxin-dependent peroxiredoxin activity
Biological Processcell redox homeostasis
Biological Processresponse to cold

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    2-Cys peroxiredoxin BAS1-like, chloroplastic
  • EC number
  • Short names
    2-Cys Prx B; 2-Cys peroxiredoxin B
  • Alternative names
    • Thiol-specific antioxidant protein B
    • Thioredoxin-dependent peroxiredoxin BAS1-like

Gene names

    • ORF names
      MHF15.19
    • Ordered locus names
      At5g06290

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9C5R8
  • Secondary accessions
    • Q9FED5
    • Q9FNH9

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

PTM/Processing

Features

Showing features for transit peptide, chain, disulfide bond.

TypeIDPosition(s)Description
Transit peptide1-72Chloroplast
ChainPRO_000028408373-2732-Cys peroxiredoxin BAS1-like, chloroplastic
Disulfide bond126Interchain (with C-248); in linked form
Disulfide bond248Interchain (with C-126); in linked form

Keywords

Proteomic databases

PTM databases

Expression

Induction

Down-regulated by ascorbate.

Gene expression databases

Interaction

Subunit

Homodimer; disulfide-linked, upon oxidation (By similarity).
Interacts with the plastidial thioredoxin CDSP32. Interacts with the plastidial NADPH-dependent thioredoxin reductase ANTR-C (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain80-239Thioredoxin

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    273
  • Mass (Da)
    29,780
  • Last updated
    2011-05-31 v3
  • Checksum
    33CB87338DADEB1B
MSMASIASSSSTTLLSSSRVLLPSKSSLLSPTVSFPRIIPSSSASSSSLCSGFSSLGSLTTNRSASRRNFAVKAQADDLPLVGNKAPDFEAEAVFDQEFIKVKLSEYIGKKYVILFFYPLDFTFVCPTEITAFSDRYEEFEKLNTEVLGVSVDSVFSHLAWVQTDRKSGGLGDLNYPLVSDITKSISKSFGVLIPDQGIALRGLFIIDKEGVIQHSTINNLGIGRSVDETMRTLQALQYVQENPDEVCPAGWKPGEKSMKPDPKLSKEYFSAI

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8BD74A0A1P8BD74_ARATH2-CYS245

Sequence caution

The sequence AAG40040.2 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.
The sequence BAB08951.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict217in Ref. 3; AAG40040

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB006700
EMBL· GenBank· DDBJ
BAB08951.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
CP002688
EMBL· GenBank· DDBJ
AED90999.1
EMBL· GenBank· DDBJ
Genomic DNA
AF324689
EMBL· GenBank· DDBJ
AAG40040.2
EMBL· GenBank· DDBJ
mRNA Different initiation
AF326871
EMBL· GenBank· DDBJ
AAG41453.1
EMBL· GenBank· DDBJ
mRNA
AF339693
EMBL· GenBank· DDBJ
AAK00375.1
EMBL· GenBank· DDBJ
mRNA
AY054621
EMBL· GenBank· DDBJ
AAK96812.1
EMBL· GenBank· DDBJ
mRNA
AY081503
EMBL· GenBank· DDBJ
AAM10065.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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