Q9C5Q8 · HEN1_ARATH
- ProteinSmall RNA 2'-O-methyltransferase
- GeneHEN1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids942 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Methyltransferase that adds a methyl group to the ribose of the last nucleotide of small RNAs (sRNAs). This protects the 3'-end of sRNAs from uridylation activity and subsequent degradation. Can methylate 3'-end of microRNAs (miRNAs), small interfering RNAs (siRNas) and trans-acting small interfering RNAs (ta-siRNAs). Involved in plant development through its role in small RNAs processing. Required for the specification of reproductive organ identities and the probable repression of A class genes. May control floral determinacy possibly by regulating the expression of the C class floral homeotic gene AGAMOUS (AG).
Catalytic activity
- S-adenosyl-L-methionine + small RNA 3'-end nucleotide = H+ + S-adenosyl-L-homocysteine + small RNA 3'-end 2'-O-methylnucleotide
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 726 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 745 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 778-779 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: SI | ||||||
Binding site | 795 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 796 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 799 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 800 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 860 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | metal ion binding | |
Molecular Function | peptidyl-prolyl cis-trans isomerase activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA methyltransferase activity | |
Molecular Function | small RNA 2'-O-methyltransferase activity | |
Biological Process | leaf proximal/distal pattern formation | |
Biological Process | leaf vascular tissue pattern formation | |
Biological Process | miRNA processing | |
Biological Process | miRNA-mediated gene silencing by mRNA destabilization | |
Biological Process | regulation of flower development | |
Biological Process | RNA methylation | |
Biological Process | RNAi-mediated antiviral immune response | |
Biological Process | specification of floral organ identity | |
Biological Process | ta-siRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSmall RNA 2'-O-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9C5Q8
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Reduced organ size, altered rosette leaf shape and increased number of coflorescences. Urydilation of miRNA 3'-ends.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 68 | In crm2-1; compact inflorescence with several flower buds at the tip. | ||||
Sequence: Missing | ||||||
Mutagenesis | 109 | No effect on RNA binding and transferase activity. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 333 | Loss of RNA binding and transferase activity. | ||||
Sequence: W → A | ||||||
Mutagenesis | 604 | No effect on transferase activity. | ||||
Sequence: L → P | ||||||
Mutagenesis | 701 | Reduces transferase activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 719 | In hen1-2; alters organ development and floral determinacy. | ||||
Sequence: D → N | ||||||
Mutagenesis | 796 | Loss of transferase activity. | ||||
Sequence: E → A | ||||||
Mutagenesis | 799 | Loss of transferase activity; when associated with A-800. | ||||
Sequence: E → A | ||||||
Mutagenesis | 800 | Loss of transferase activity; when associated with A-799. | ||||
Sequence: H → A | ||||||
Mutagenesis | 800 | Strongly decreases transferase activity. | ||||
Sequence: H → Q | ||||||
Mutagenesis | 856 | Reduces transferase activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 860 | Loss of transferase activity. | ||||
Sequence: H → A or Q |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 127 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000404658 | 1-942 | Small RNA 2'-O-methyltransferase | |||
Sequence: MAGGGKHTPTPKAIIHQKFGAKASYTVEEVHDSSQSGCLGLAIPQKGPCLYRCHLQLPEFSVVSNVFKKKKDSEQSAAELALDKLGIRPQNDDLTVDEARDEIVGRIKYIFSDEFLSAEHPLGAHLRAALRRDGERCGSVPVSVIATVDAKINSRCKIINPSVESDPFLAISYVMKAAAKLADYIVASPHGLRRKNAYPSEIVEALATHVSDSLHSREVAAVYIPCIDEEVVELDTLYISSNRHYLDSIAERLGLKDGNQVMISRMFGKASCGSECRLYSEIPKKYLDNSSDASGTSNEDSSHIVKSRNARASYICGQDIHGDAILASVGYRWKSDDLDYDDVTVNSFYRICCGMSPNGIYKISRQAVIAAQLPFAFTTKSNWRGPLPREILGLFCHQHRLAEPILSSSTAPVKSLSDIFRSHKKLKVSGVDDANENLSRQKEDTPGLGHGFRCEVKIFTKSQDLVLECSPRKFYEKENDAIQNASLKALLWFSKFFADLDVDGEQSCDTDDDQDTKSSSPNVFAAPPILQKEHSSESKNTNVLSAEKRVQSITNGSVVSICYSLSLAVDPEYSSDGESPREDNESNEEMESEYSANCESSVELIESNEEIEFEVGTGSMNPHIESEVTQMTVGEYASFRMTPPDAAEALILAVGSDTVRIRSLLSERPCLNYNILLLGVKGPSEERMEAAFFKPPLSKQRVEYALKHIRESSASTLVDFGCGSGSLLDSLLDYPTSLQTIIGVDISPKGLARAAKMLHVKLNKEACNVKSATLYDGSILEFDSRLHDVDIGTCLEVIEHMEEDQACEFGEKVLSLFHPKLLIVSTPNYEFNTILQRSTPETQEENNSEPQLPKFRNHDHKFEWTREQFNQWASKLGKRHNYSVEFSGVGGSGEVEPGFASQIAIFRREASSVENVAESSMQPYKVIWEWKKEDVEKKKTDL |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in stems, leaves and inflorescences.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 19-87 | DRBM | ||||
Sequence: FGAKASYTVEEVHDSSQSGCLGLAIPQKGPCLYRCHLQLPEFSVVSNVFKKKKDSEQSAAELALDKLGI | ||||||
Domain | 93-204 | HTH La-type RNA-binding | ||||
Sequence: DLTVDEARDEIVGRIKYIFSDEFLSAEHPLGAHLRAALRRDGERCGSVPVSVIATVDAKINSRCKIINPSVESDPFLAISYVMKAAAKLADYIVASPHGLRRKNAYPSEIVE | ||||||
Region | 505-542 | Disordered | ||||
Sequence: EQSCDTDDDQDTKSSSPNVFAAPPILQKEHSSESKNTN | ||||||
Region | 571-596 | Disordered | ||||
Sequence: PEYSSDGESPREDNESNEEMESEYSA | ||||||
Region | 837-856 | Disordered | ||||
Sequence: RSTPETQEENNSEPQLPKFR |
Sequence similarities
Belongs to the methyltransferase superfamily. HEN1 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length942
- Mass (Da)104,454
- Last updated2001-06-01 v1
- Checksum7D8B5222C0D28779
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 39 | in Ref. 1; AAC97105 and 2; AAL05056 | ||||
Sequence: L → P | ||||||
Sequence conflict | 552 | in Ref. 1; AAC97105 | ||||
Sequence: S → Y | ||||||
Sequence conflict | 660 | in Ref. 1; AAC97105 | ||||
Sequence: R → K |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF060248 EMBL· GenBank· DDBJ | AAC97105.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF411383 EMBL· GenBank· DDBJ | AAL05056.1 EMBL· GenBank· DDBJ | mRNA | ||
AF327068 EMBL· GenBank· DDBJ | AAK16435.1 EMBL· GenBank· DDBJ | mRNA | ||
AL080282 EMBL· GenBank· DDBJ | CAB45886.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AL161553 EMBL· GenBank· DDBJ | CAB79091.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002687 EMBL· GenBank· DDBJ | AEE84374.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE84375.1 EMBL· GenBank· DDBJ | Genomic DNA |