Q9C5Q8 · HEN1_ARATH

Function

function

Methyltransferase that adds a methyl group to the ribose of the last nucleotide of small RNAs (sRNAs). This protects the 3'-end of sRNAs from uridylation activity and subsequent degradation. Can methylate 3'-end of microRNAs (miRNAs), small interfering RNAs (siRNas) and trans-acting small interfering RNAs (ta-siRNAs). Involved in plant development through its role in small RNAs processing. Required for the specification of reproductive organ identities and the probable repression of A class genes. May control floral determinacy possibly by regulating the expression of the C class floral homeotic gene AGAMOUS (AG).

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site726S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site745S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site778-779S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site795S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site796Mg2+ (UniProtKB | ChEBI)
Binding site799Mg2+ (UniProtKB | ChEBI)
Binding site800Mg2+ (UniProtKB | ChEBI)
Binding site860Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Molecular Functionmetal ion binding
Molecular Functionpeptidyl-prolyl cis-trans isomerase activity
Molecular FunctionRNA binding
Molecular FunctionRNA methyltransferase activity
Molecular Functionsmall RNA 2'-O-methyltransferase activity
Biological Processleaf proximal/distal pattern formation
Biological Processleaf vascular tissue pattern formation
Biological ProcessmiRNA processing
Biological ProcessmiRNA-mediated gene silencing by mRNA destabilization
Biological Processregulation of flower development
Biological ProcessRNA methylation
Biological ProcessRNAi-mediated antiviral immune response
Biological Processspecification of floral organ identity
Biological Processta-siRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Small RNA 2'-O-methyltransferase
  • EC number
  • Alternative names
    • Protein CORYMBOSA 2
    • Protein HUA ENHANCER 1
    • S-adenosylmethionine-dependent RNA methyltransferase HEN1

Gene names

    • Name
      HEN1
    • Synonyms
      CRM2
    • ORF names
      T13K14.70
    • Ordered locus names
      At4g20910

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Wassilewskija
    • cv. Landsberg erecta
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9C5Q8
  • Secondary accessions
    • O65309
    • Q945R3
    • Q9SUC2

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Reduced organ size, altered rosette leaf shape and increased number of coflorescences. Urydilation of miRNA 3'-ends.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis68In crm2-1; compact inflorescence with several flower buds at the tip.
Mutagenesis109No effect on RNA binding and transferase activity.
Mutagenesis333Loss of RNA binding and transferase activity.
Mutagenesis604No effect on transferase activity.
Mutagenesis701Reduces transferase activity.
Mutagenesis719In hen1-2; alters organ development and floral determinacy.
Mutagenesis796Loss of transferase activity.
Mutagenesis799Loss of transferase activity; when associated with A-800.
Mutagenesis800Loss of transferase activity; when associated with A-799.
Mutagenesis800Strongly decreases transferase activity.
Mutagenesis856Reduces transferase activity.
Mutagenesis860Loss of transferase activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 127 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004046581-942Small RNA 2'-O-methyltransferase

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in stems, leaves and inflorescences.

Gene expression databases

Interaction

Subunit

Binds small RNA duplexes as monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain19-87DRBM
Domain93-204HTH La-type RNA-binding
Region505-542Disordered
Region571-596Disordered
Region837-856Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    942
  • Mass (Da)
    104,454
  • Last updated
    2001-06-01 v1
  • Checksum
    7D8B5222C0D28779
MAGGGKHTPTPKAIIHQKFGAKASYTVEEVHDSSQSGCLGLAIPQKGPCLYRCHLQLPEFSVVSNVFKKKKDSEQSAAELALDKLGIRPQNDDLTVDEARDEIVGRIKYIFSDEFLSAEHPLGAHLRAALRRDGERCGSVPVSVIATVDAKINSRCKIINPSVESDPFLAISYVMKAAAKLADYIVASPHGLRRKNAYPSEIVEALATHVSDSLHSREVAAVYIPCIDEEVVELDTLYISSNRHYLDSIAERLGLKDGNQVMISRMFGKASCGSECRLYSEIPKKYLDNSSDASGTSNEDSSHIVKSRNARASYICGQDIHGDAILASVGYRWKSDDLDYDDVTVNSFYRICCGMSPNGIYKISRQAVIAAQLPFAFTTKSNWRGPLPREILGLFCHQHRLAEPILSSSTAPVKSLSDIFRSHKKLKVSGVDDANENLSRQKEDTPGLGHGFRCEVKIFTKSQDLVLECSPRKFYEKENDAIQNASLKALLWFSKFFADLDVDGEQSCDTDDDQDTKSSSPNVFAAPPILQKEHSSESKNTNVLSAEKRVQSITNGSVVSICYSLSLAVDPEYSSDGESPREDNESNEEMESEYSANCESSVELIESNEEIEFEVGTGSMNPHIESEVTQMTVGEYASFRMTPPDAAEALILAVGSDTVRIRSLLSERPCLNYNILLLGVKGPSEERMEAAFFKPPLSKQRVEYALKHIRESSASTLVDFGCGSGSLLDSLLDYPTSLQTIIGVDISPKGLARAAKMLHVKLNKEACNVKSATLYDGSILEFDSRLHDVDIGTCLEVIEHMEEDQACEFGEKVLSLFHPKLLIVSTPNYEFNTILQRSTPETQEENNSEPQLPKFRNHDHKFEWTREQFNQWASKLGKRHNYSVEFSGVGGSGEVEPGFASQIAIFRREASSVENVAESSMQPYKVIWEWKKEDVEKKKTDL

Sequence caution

The sequence CAB45886.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence CAB79091.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict39in Ref. 1; AAC97105 and 2; AAL05056
Sequence conflict552in Ref. 1; AAC97105
Sequence conflict660in Ref. 1; AAC97105

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF060248
EMBL· GenBank· DDBJ
AAC97105.1
EMBL· GenBank· DDBJ
Genomic DNA
AF411383
EMBL· GenBank· DDBJ
AAL05056.1
EMBL· GenBank· DDBJ
mRNA
AF327068
EMBL· GenBank· DDBJ
AAK16435.1
EMBL· GenBank· DDBJ
mRNA
AL080282
EMBL· GenBank· DDBJ
CAB45886.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AL161553
EMBL· GenBank· DDBJ
CAB79091.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002687
EMBL· GenBank· DDBJ
AEE84374.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002687
EMBL· GenBank· DDBJ
AEE84375.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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