Q9C512 · MNS1_ARATH

Function

function

Class I alpha-mannosidase essential for early N-glycan processing. Progressively trims alpha-1,2-linked mannose residues. Produces Man5GlcNAc2 from Man8GlcNAc2, but only Man6GlcNAc2 from Man9GlcNAc2. Has difficulty acting on the terminal mannose of the b-branch. Involved in root development and cell wall biosynthesis.

Catalytic activity

Cofactor

Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Ca2+ or Mn2+. Mg2+ can be used to a lesser extent.

Activity regulation

Inhibited by kifunensine and 1-deoxymannojirimycin, but not by swainsonine.

pH Dependence

Optimum pH is 6.0. Stable from pH 4.5 to 6.5.

Temperature Dependence

Optimum temperature is 25 degrees Celsius.

Pathway

Protein modification; protein glycosylation.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site179Proton donor
Active site312
Active site423Proton donor
Active site445
Binding site529Ca2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentendosome
Cellular ComponentGolgi apparatus
Cellular ComponentGolgi cis cisterna
Cellular ComponentGolgi membrane
Cellular Componenttrans-Golgi network
Molecular Functionalpha-mannosidase activity
Molecular Functioncalcium ion binding
Molecular Functionmannosyl-oligosaccharide 1,2-alpha-mannosidase activity
Biological Processcarbohydrate metabolic process
Biological ProcessN-glycan processing
Biological Processprotein glycosylation

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1
  • EC number
  • Short names
    AtMANIb
  • Alternative names
    • Alpha-mannosidase IB

Gene names

    • Name
      MNS1
    • Synonyms
      MANIB
    • ORF names
      F19C24.18, F5D21.1
    • Ordered locus names
      At1g51590

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9C512
  • Secondary accessions
    • Q2V4H4

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-27Cytoplasmic
Transmembrane28-47Helical; Signal-anchor for type II membrane protein
Topological domain48-560Lumenal

Keywords

Phenotypes & Variants

Disruption phenotype

No visible phenotype; due the redundancy with MNS2. Lack of complex N-glycans, shorter roots and increased lateral root formation in mns1 and mns2 double mutants.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 21 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, glycosylation, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00003979331-560Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1
Glycosylation326N-linked (GlcNAc...) asparagine
Disulfide bond377↔409
Glycosylation459N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in flowers, siliques, stems, leaves, roots, pollen grains, shoot apical meristems, hypocotyls and upper region of the root.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for coiled coil.

TypeIDPosition(s)Description
Coiled coil47-80

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q9C512-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    560
  • Mass (Da)
    63,532
  • Last updated
    2001-06-01 v1
  • Checksum
    BCC20CB9E63020B9
MARSRSISGYGIWKYLNPAYYLRRPRRLALLFIVFVSVSMLVWDRINLAREHEVEVFKLNEEVSRLEQMLEELNGGVGNKPLKTLKDAPEDPVDKQRRQKVKEAMIHAWSSYEKYAWGKDELQPRTKDGTDSFGGLGATMVDSLDTLYIMGLDEQFQKAREWVASSLDFDKDYDASMFETTIRVVGGLLSAYDLSGDKMFLEKAKDIADRLLPAWNTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGTEQLEFIALSQRTGDPKYQQKVEKVITELNKNFPADGLLPIYINPDNANPSYSTTTFGAMGDSFYEYLLKVWVQGNKTSAVKPYRDMWEKSMKGLLSLVKKSTPSSFTYICEKNGNNLIDKMDELACFAPGMLALGASGYGPDEEKKFLSLAGELAWTCYNFYQSTPTKLAGENYFFTAGQDMSVGTSWNILRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRVESGYVGLKDVNTGAKDNKMQSFFLAETLKYLYLLFSPSSVISLDEWVFNTEAHPLKIVARNDPRKPTIALRQRKFGHQINV

Q9C512-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0397241-104in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC024261
EMBL· GenBank· DDBJ
AAG52623.1
EMBL· GenBank· DDBJ
Genomic DNA
AC025294
EMBL· GenBank· DDBJ
AAG50876.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE32686.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE32687.1
EMBL· GenBank· DDBJ
Genomic DNA
AY081353
EMBL· GenBank· DDBJ
AAL91242.1
EMBL· GenBank· DDBJ
mRNA
AY128845
EMBL· GenBank· DDBJ
AAM91245.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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