Q9BY07 · S4A5_HUMAN
- ProteinElectrogenic sodium bicarbonate cotransporter 4
- GeneSLC4A5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1137 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Mediates sodium- and bicarbonate-dependent electrogenic sodium bicarbonate cotransport, with a Na+:HCO3- stoichiometry varying from 1:2 to 1:3.
Catalytic activity
- 2 hydrogencarbonate(out) + Na+(out) = 2 hydrogencarbonate(in) + Na+(in)
2 hydrogencarbonate (out)CHEBI:17544+ Na+ (out)CHEBI:29101= 2 hydrogencarbonate (in)CHEBI:17544+ Na+ (in)CHEBI:29101 - 3 hydrogencarbonate(out) + Na+(out) = 3 hydrogencarbonate(in) + Na+(in)
Isoform 3
2 hydrogencarbonate(out) + Na+(out) = 2 hydrogencarbonate(in) + Na+(in)2 hydrogencarbonate (out)CHEBI:17544+ Na+ (out)CHEBI:29101= 2 hydrogencarbonate (in)CHEBI:17544+ Na+ (in)CHEBI:29101Isoform 3
3 hydrogencarbonate(out) + Na+(out) = 3 hydrogencarbonate(in) + Na+(in)Isoform 8
2 hydrogencarbonate(out) + Na+(out) = 2 hydrogencarbonate(in) + Na+(in)2 hydrogencarbonate (out)CHEBI:17544+ Na+ (out)CHEBI:29101= 2 hydrogencarbonate (in)CHEBI:17544+ Na+ (in)CHEBI:29101Isoform 8
3 hydrogencarbonate(out) + Na+(out) = 3 hydrogencarbonate(in) + Na+(in)
Activity regulation
Activity is inhibited by 4,4'-di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS - an inhibitor of several anion channels and transporters).
Isoform 3
Activity is inhibited by 4,4'-di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS - an inhibitor of several anion channels and transporters).
Isoform 8
Activity is inhibited by 4,4'-di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS - an inhibitor of several anion channels and transporters).
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | basolateral plasma membrane | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | monoatomic anion transmembrane transporter activity | |
Molecular Function | sodium:bicarbonate symporter activity | |
Molecular Function | solute:inorganic anion antiporter activity | |
Biological Process | bicarbonate transport | |
Biological Process | cerebrospinal fluid secretion | |
Biological Process | epithelial cell development | |
Biological Process | mitochondrion distribution | |
Biological Process | regulation of gene expression | |
Biological Process | regulation of intracellular pH | |
Biological Process | regulation of systemic arterial blood pressure | |
Biological Process | renal system process | |
Biological Process | retina development in camera-type eye | |
Biological Process | transmembrane transport |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameElectrogenic sodium bicarbonate cotransporter 4
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ9BY07
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Apical cell membrane ; Multi-pass membrane protein
Basolateral cell membrane ; Multi-pass membrane protein
Note: Expressed in the apical plasma membrane domain of a subset of collecting ducts in the renal medulla.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-521 | Cytoplasmic | ||||
Sequence: MKVKEEKAGVGKLDHTNHRRRFPDQKECPPIHIGLPVPTYPQRKTDQKGHLSGLQKVHWGLRPDQPQQELTGPGSGASSQDSSMDLISRTRSPAAEQLQDILGEEDEAPNPTLFTEMDTLQHDGDQMEWKESARWIKFEEKVEEGGERWSKPHVSTLSLHSLFELRTCLQTGTVLLDLDSGSLPQIIDDVIEKQIEDGLLRPELRERVSYVLLRRHRHQTKKPIHRSLADIGKSVSTTNRSPARSPGAGPSLHHSTEDLRMRQSANYGRLCHAQSRSMNDISLTPNTDQRKNKFMKKIPKDSEASNVLVGEVDFLDQPFIAFVRLIQSAMLGGVTEVPVPTRFLFILLGPSGRAKSYNEIGRAIATLMVDDLFSDVAYKARNREDLIAGIDEFLDEVIVLPPGEWDPNIRIEPPKKVPSADKRKSVFSLAELGQMNGSVGGGGGAPGGGNGGGGGGGSGGGAGSGGAGGTSSGDDGEMPAMHEIGEELIWTGRFFGGLCLDIKRKLPWFPSDFYDGFHIQS | ||||||
Transmembrane | 522-544 | Helical | ||||
Sequence: ISAILFIYLGCITNAITFGGLLG | ||||||
Topological domain | 545-555 | Extracellular | ||||
Sequence: DATDNYQGVME | ||||||
Transmembrane | 556-587 | Helical | ||||
Sequence: SFLGTAMAGSLFCLFSGQPLIILSSTGPILIF | ||||||
Topological domain | 588-606 | Cytoplasmic | ||||
Sequence: EKLLFDFSKGNGLDYMEFR | ||||||
Transmembrane | 607-628 | Helical | ||||
Sequence: LWIGLHSAVQCLILVATDASFI | ||||||
Topological domain | 629-742 | Extracellular | ||||
Sequence: IKYITRFTEEGFSTLISFIFIYDAIKKMIGAFKYYPINMDFKPNFITTYKCECVAPDTVNTTVFNASAPLAPDTNASLYNLLNLTALDWSLLSKKECLSYGGRLLGNSCKFIPD | ||||||
Transmembrane | 743-762 | Helical | ||||
Sequence: LALMSFILFFGTYSMTLTLK | ||||||
Topological domain | 763-773 | Cytoplasmic | ||||
Sequence: KFKFSRYFPTK | ||||||
Transmembrane | 774-795 | Helical | ||||
Sequence: VRALVADFSIVFSILMFCGIDA | ||||||
Topological domain | 796-828 | Extracellular | ||||
Sequence: CFGLETPKLHVPSVIKPTRPDRGWFVAPFGKNP | ||||||
Transmembrane | 829-847 | Helical | ||||
Sequence: WWVYPASILPALLVTILIF | ||||||
Topological domain | 848-866 | Cytoplasmic | ||||
Sequence: MDQQITAVIVNRKENKLKK | ||||||
Transmembrane | 867-883 | Helical | ||||
Sequence: AAGYHLDLFWVGILMAL | ||||||
Topological domain | 884-888 | Extracellular | ||||
Sequence: CSFMG | ||||||
Transmembrane | 889-908 | Helical | ||||
Sequence: LPWYVAATVISIAHIDSLKM | ||||||
Topological domain | 909-928 | Cytoplasmic | ||||
Sequence: ETETSAPGEQPQFLGVREQR | ||||||
Transmembrane | 929-948 | Helical | ||||
Sequence: VTGIIVFILTGISVFLAPIL | ||||||
Topological domain | 949-953 | Extracellular | ||||
Sequence: KCIPL | ||||||
Transmembrane | 954-971 | Helical | ||||
Sequence: PVLYGVFLYMGVASLNGI | ||||||
Topological domain | 972-1016 | Cytoplasmic | ||||
Sequence: QMGTGGSEFKIQKKLTPFWERCKLFLMPAKHQPDHAFLRHVPLRR | ||||||
Transmembrane | 1017-1034 | Helical | ||||
Sequence: IHLFTLVQILCLAVLWIL | ||||||
Topological domain | 1035-1039 | Extracellular | ||||
Sequence: KSTVA | ||||||
Transmembrane | 1040-1057 | Helical | ||||
Sequence: AIIFPVMILGLIIVRRLL | ||||||
Topological domain | 1058-1137 | Cytoplasmic | ||||
Sequence: DFIFSQHDLAWIDNILPEKEKKETDKKRKRKKGAHEDCDEEPQFPPPSVIKIPMESVQSDPQNGIHCIARKRSSSWSYSL |
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_048350 | 251 | in dbSNP:rs17009792 | |||
Sequence: S → N | ||||||
Natural variant | VAR_061032 | 253 | in dbSNP:rs55651232 | |||
Sequence: H → Y |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1,377 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000328920 | 1-1137 | Electrogenic sodium bicarbonate cotransporter 4 | |||
Sequence: MKVKEEKAGVGKLDHTNHRRRFPDQKECPPIHIGLPVPTYPQRKTDQKGHLSGLQKVHWGLRPDQPQQELTGPGSGASSQDSSMDLISRTRSPAAEQLQDILGEEDEAPNPTLFTEMDTLQHDGDQMEWKESARWIKFEEKVEEGGERWSKPHVSTLSLHSLFELRTCLQTGTVLLDLDSGSLPQIIDDVIEKQIEDGLLRPELRERVSYVLLRRHRHQTKKPIHRSLADIGKSVSTTNRSPARSPGAGPSLHHSTEDLRMRQSANYGRLCHAQSRSMNDISLTPNTDQRKNKFMKKIPKDSEASNVLVGEVDFLDQPFIAFVRLIQSAMLGGVTEVPVPTRFLFILLGPSGRAKSYNEIGRAIATLMVDDLFSDVAYKARNREDLIAGIDEFLDEVIVLPPGEWDPNIRIEPPKKVPSADKRKSVFSLAELGQMNGSVGGGGGAPGGGNGGGGGGGSGGGAGSGGAGGTSSGDDGEMPAMHEIGEELIWTGRFFGGLCLDIKRKLPWFPSDFYDGFHIQSISAILFIYLGCITNAITFGGLLGDATDNYQGVMESFLGTAMAGSLFCLFSGQPLIILSSTGPILIFEKLLFDFSKGNGLDYMEFRLWIGLHSAVQCLILVATDASFIIKYITRFTEEGFSTLISFIFIYDAIKKMIGAFKYYPINMDFKPNFITTYKCECVAPDTVNTTVFNASAPLAPDTNASLYNLLNLTALDWSLLSKKECLSYGGRLLGNSCKFIPDLALMSFILFFGTYSMTLTLKKFKFSRYFPTKVRALVADFSIVFSILMFCGIDACFGLETPKLHVPSVIKPTRPDRGWFVAPFGKNPWWVYPASILPALLVTILIFMDQQITAVIVNRKENKLKKAAGYHLDLFWVGILMALCSFMGLPWYVAATVISIAHIDSLKMETETSAPGEQPQFLGVREQRVTGIIVFILTGISVFLAPILKCIPLPVLYGVFLYMGVASLNGIQMGTGGSEFKIQKKLTPFWERCKLFLMPAKHQPDHAFLRHVPLRRIHLFTLVQILCLAVLWILKSTVAAIIFPVMILGLIIVRRLLDFIFSQHDLAWIDNILPEKEKKETDKKRKRKKGAHEDCDEEPQFPPPSVIKIPMESVQSDPQNGIHCIARKRSSSWSYSL |
Proteomic databases
PTM databases
Expression
Tissue specificity
Highest expression observed in liver, spleen and testis; moderate expression in the choroid plexus, hippocampus, cerebrum and cerebellum of brain, and in kidney cortex and kidney medulla. Also observed in heart, pancreas, muscle, lung, placenta, stomach and small intestine. Weakest expression seen in peripheral blood lymphocytes, colon, duodenum, jejunum, ileum and skeletal muscle.
Isoform 3
Expressed in the brain, heart, kidney, testis, pancreas, muscle and peripheral blood leukocytes.
Gene expression databases
Organism-specific databases
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-27 | Basic and acidic residues | ||||
Sequence: MKVKEEKAGVGKLDHTNHRRRFPDQKE | ||||||
Region | 1-52 | Disordered | ||||
Sequence: MKVKEEKAGVGKLDHTNHRRRFPDQKECPPIHIGLPVPTYPQRKTDQKGHLS | ||||||
Region | 64-94 | Disordered | ||||
Sequence: DQPQQELTGPGSGASSQDSSMDLISRTRSPA | ||||||
Compositional bias | 68-94 | Polar residues | ||||
Sequence: QELTGPGSGASSQDSSMDLISRTRSPA | ||||||
Region | 221-255 | Disordered | ||||
Sequence: KKPIHRSLADIGKSVSTTNRSPARSPGAGPSLHHS | ||||||
Compositional bias | 231-245 | Polar residues | ||||
Sequence: IGKSVSTTNRSPARS | ||||||
Region | 438-478 | Disordered | ||||
Sequence: SVGGGGGAPGGGNGGGGGGGSGGGAGSGGAGGTSSGDDGEM | ||||||
Compositional bias | 1079-1099 | Basic and acidic residues | ||||
Sequence: KETDKKRKRKKGAHEDCDEEP | ||||||
Region | 1079-1137 | Disordered | ||||
Sequence: KETDKKRKRKKGAHEDCDEEPQFPPPSVIKIPMESVQSDPQNGIHCIARKRSSSWSYSL | ||||||
Compositional bias | 1112-1137 | Polar residues | ||||
Sequence: ESVQSDPQNGIHCIARKRSSSWSYSL |
Sequence similarities
Belongs to the anion exchanger (TC 2.A.31) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 8 isoforms produced by Alternative splicing.
Q9BY07-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsNBC4a
- Length1,137
- Mass (Da)126,255
- Last updated2008-04-08 v2
- ChecksumED4E4A4DC2CA17F1
Q9BY07-2
- Name2
- SynonymsNBC4b
- Differences from canonical
- 1046-1046: M → MSLSTTD
Q9BY07-3
- Name3
- SynonymsNBC4c
- Differences from canonical
- 973-988: Missing
Q9BY07-4
- Name4
- SynonymsNBC4d
- Differences from canonical
- 950-1046: Missing
Q9BY07-5
- Name5
- Synonymsisoform d variant
- NoteIncomplete sequence.
- Differences from canonical
- 88-91: SRTR → EHKG
- 866-993: KAAGYHLDLFWVGILMALCSFMGLPWYVAATVISIAHIDSLKMETETSAPGEQPQFLGVREQRVTGIIVFILTGISVFLAPILKCIPLPVLYGVFLYMGVASLNGIQMGTGGSEFKIQKKLTPFWERC → GTESNRHHRLHPDGNLCLPGSHPKVYPPAGAVRSLPLHGRGLPEWHPVLGTLQALPDASQAPAGPCLPAARAAAPDPPLHPGADPLPGGALDPQIHGGCHHLPGHDPGPHHRSKASGFHLFPARPGLD
- 994-1137: Missing
Q9BY07-6
- Name6
- SynonymsNBC4f
- Differences from canonical
- 774-811: Missing
- 866-924: Missing
- 973-988: Missing
- 1046-1046: M → MSLSTTD
- 1071-1075: NILPE → KIEFP
- 1076-1137: Missing
Q9BY07-7
- Name7
Q9BY07-8
- Name8
- SynonymsNBC4e
- Differences from canonical
- 1068-1137: Missing
Computationally mapped potential isoform sequences
There are 6 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
E7EQT3 | E7EQT3_HUMAN | SLC4A5 | 998 | ||
C9JU12 | C9JU12_HUMAN | SLC4A5 | 144 | ||
C9JW32 | C9JW32_HUMAN | SLC4A5 | 129 | ||
C9JNL6 | C9JNL6_HUMAN | SLC4A5 | 122 | ||
A0A1B0GUV9 | A0A1B0GUV9_HUMAN | SLC4A5 | 71 | ||
A0A1B0GVY3 | A0A1B0GVY3_HUMAN | SLC4A5 | 210 |
Sequence caution
Features
Showing features for compositional bias, alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-27 | Basic and acidic residues | ||||
Sequence: MKVKEEKAGVGKLDHTNHRRRFPDQKE | ||||||
Alternative sequence | VSP_052770 | 27-91 | in isoform 7 | |||
Sequence: ECPPIHIGLPVPTYPQRKTDQKGHLSGLQKVHWGLRPDQPQQELTGPGSGASSQDSSMDLISRTR → G | ||||||
Compositional bias | 68-94 | Polar residues | ||||
Sequence: QELTGPGSGASSQDSSMDLISRTRSPA | ||||||
Alternative sequence | VSP_052771 | 88-91 | in isoform 5 | |||
Sequence: SRTR → EHKG | ||||||
Compositional bias | 231-245 | Polar residues | ||||
Sequence: IGKSVSTTNRSPARS | ||||||
Sequence conflict | 612 | in Ref. 6; AAL48291/AAL50802 | ||||
Sequence: H → Y | ||||||
Sequence conflict | 699 | in Ref. 6; AAL48291 | ||||
Sequence: A → T | ||||||
Sequence conflict | 759 | in Ref. 6; AAL50802 | ||||
Sequence: L → R | ||||||
Alternative sequence | VSP_052772 | 774-811 | in isoform 6 and isoform 7 | |||
Sequence: Missing | ||||||
Sequence conflict | 813 | in Ref. 2; AAK26741 | ||||
Sequence: T → P | ||||||
Alternative sequence | VSP_052774 | 866-924 | in isoform 6 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_052773 | 866-993 | in isoform 5 | |||
Sequence: KAAGYHLDLFWVGILMALCSFMGLPWYVAATVISIAHIDSLKMETETSAPGEQPQFLGVREQRVTGIIVFILTGISVFLAPILKCIPLPVLYGVFLYMGVASLNGIQMGTGGSEFKIQKKLTPFWERC → GTESNRHHRLHPDGNLCLPGSHPKVYPPAGAVRSLPLHGRGLPEWHPVLGTLQALPDASQAPAGPCLPAARAAAPDPPLHPGADPLPGGALDPQIHGGCHHLPGHDPGPHHRSKASGFHLFPARPGLD | ||||||
Sequence conflict | 896 | in Ref. 6; AAL48291 | ||||
Sequence: T → M | ||||||
Sequence conflict | 923 | in Ref. 6; AAL48291 | ||||
Sequence: G → R | ||||||
Alternative sequence | VSP_052775 | 950-1046 | in isoform 4 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_052776 | 973-988 | in isoform 3, isoform 6 and isoform 7 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_052777 | 994-1137 | in isoform 5 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_052778 | 1046 | in isoform 2 and isoform 6 | |||
Sequence: M → MSLSTTD | ||||||
Alternative sequence | VSP_052779 | 1068-1137 | in isoform 8 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_052780 | 1071-1075 | in isoform 6 | |||
Sequence: NILPE → KIEFP | ||||||
Alternative sequence | VSP_052781 | 1076-1137 | in isoform 6 | |||
Sequence: Missing | ||||||
Compositional bias | 1079-1099 | Basic and acidic residues | ||||
Sequence: KETDKKRKRKKGAHEDCDEEP | ||||||
Compositional bias | 1112-1137 | Polar residues | ||||
Sequence: ESVQSDPQNGIHCIARKRSSSWSYSL |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF207661 EMBL· GenBank· DDBJ | AAG18492.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AF243499 EMBL· GenBank· DDBJ | AAK26741.1 EMBL· GenBank· DDBJ | mRNA | ||
AF293338 EMBL· GenBank· DDBJ | AAK97073.1 EMBL· GenBank· DDBJ | mRNA | ||
AF293337 EMBL· GenBank· DDBJ | AAK97072.1 EMBL· GenBank· DDBJ | mRNA | ||
AF452248 EMBL· GenBank· DDBJ | AAL48291.1 EMBL· GenBank· DDBJ | mRNA | ||
AF453528 EMBL· GenBank· DDBJ | AAL50802.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
CH471053 EMBL· GenBank· DDBJ | EAW99690.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471053 EMBL· GenBank· DDBJ | EAW99691.1 EMBL· GenBank· DDBJ | Genomic DNA | Frameshift | |
CH471053 EMBL· GenBank· DDBJ | EAW99693.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC109221 EMBL· GenBank· DDBJ | AAI09222.1 EMBL· GenBank· DDBJ | mRNA | ||
AB209752 EMBL· GenBank· DDBJ | BAD92989.1 EMBL· GenBank· DDBJ | mRNA |