Q9BRQ3 · NUD22_HUMAN
- ProteinUridine diphosphate glucose pyrophosphatase NUDT22
- GeneNUDT22
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids303 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Hydrolyzes UDP-glucose to glucose 1-phosphate and UMP and UDP-galactose to galactose 1-phosphate and UMP. Preferred substrate is UDP-glucose.
Catalytic activity
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
16.5 μM | UDP-glucose | |||||
51.4 μM | UDP-galactose |
kcat is 0.69 sec-1 for UDP-glucose. kcat is 0.55 sec-1 for UDP-galactose.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 56 | substrate | ||||
Sequence: F | ||||||
Binding site | 87 | substrate | ||||
Sequence: Y | ||||||
Binding site | 139 | substrate | ||||
Sequence: R | ||||||
Binding site | 144 | substrate | ||||
Sequence: A | ||||||
Binding site | 151 | substrate | ||||
Sequence: D | ||||||
Binding site | 156 | substrate | ||||
Sequence: H | ||||||
Binding site | 158 | substrate | ||||
Sequence: E | ||||||
Binding site | 189 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 193 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 274 | substrate | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleoplasm | |
Molecular Function | GDP-mannose hydrolase activity | |
Molecular Function | metal ion binding | |
Molecular Function | UDP-sugar diphosphatase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUridine diphosphate glucose pyrophosphatase NUDT22
- EC number
- Short namesUDPG pyrophosphatase; UGPPase
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ9BRQ3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Disease & Variants
Features
Showing features for natural variant, mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_029616 | 36 | in dbSNP:rs2286612 | |||
Sequence: G → C | ||||||
Natural variant | VAR_050414 | 129 | in dbSNP:rs34448455 | |||
Sequence: T → R | ||||||
Mutagenesis | 156 | Abolishes enzyme activity with UDP-galactose. | ||||
Sequence: H → A | ||||||
Mutagenesis | 189 | Abolishes enzyme activity with UDP-galactose. | ||||
Sequence: E → A | ||||||
Mutagenesis | 193 | Abolishes enzyme activity with UDP-galactose. | ||||
Sequence: E → A | ||||||
Natural variant | VAR_029617 | 260 | in dbSNP:rs633561 | |||
Sequence: Q → R | ||||||
Natural variant | VAR_029618 | 263 | in dbSNP:rs633557 | |||
Sequence: L → P |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 355 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000263731 | 1-303 | UniProt | Uridine diphosphate glucose pyrophosphatase NUDT22 | |||
Sequence: MDPEVTLLLQCPGGGLPQEQIQAELSPAHDRRPLPGGDEAITAIWETRLKAQPWLFDAPKFRLHSATLAPIGSRGPQLLLRLGLTSYRDFLGTNWSSSAAWLRQQGATDWGDTQAYLADPLGVGAALATADDFLVFLRRSRQVAEAPGLVDVPGGHPEPQALCPGGSPQHQDLAGQLVVHELFSSVLQEICDEVNLPLLTLSQPLLLGIARNETSAGRASAEFYVQCSLTSEQVRKHYLSGGPEAHESTGIFFVETQNVQRLLETEMWAELCPSAKGAIILYNRVQGSPTGAALGSPALLPPL | |||||||
Modified residue (large scale data) | 296 | PRIDE | Phosphoserine | ||||
Sequence: S |
Proteomic databases
PTM databases
Expression
Interaction
Binary interactions
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 118-285 | Nudix hydrolase | ||||
Sequence: ADPLGVGAALATADDFLVFLRRSRQVAEAPGLVDVPGGHPEPQALCPGGSPQHQDLAGQLVVHELFSSVLQEICDEVNLPLLTLSQPLLLGIARNETSAGRASAEFYVQCSLTSEQVRKHYLSGGPEAHESTGIFFVETQNVQRLLETEMWAELCPSAKGAIILYNRV | ||||||
Region | 148-168 | Disordered | ||||
Sequence: GLVDVPGGHPEPQALCPGGSP | ||||||
Motif | 175-196 | Nudix box | ||||
Sequence: GQLVVHELFSSVLQEICDEVNL |
Sequence similarities
Belongs to the Nudix hydrolase family.
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q9BRQ3-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length303
- Mass (Da)32,580
- Last updated2011-01-11 v3
- ChecksumC26958E8BB90B02F
Q9BRQ3-2
- Name2
- Differences from canonical
- 161-303: ALCPGGSPQHQDLAGQLVVHELFSSVLQEICDEVNLPLLTLSQPLLLGIARNETSAGRASAEFYVQCSLTSEQVRKHYLSGGPEAHESTGIFFVETQNVQRLLETEMWAELCPSAKGAIILYNRVQGSPTGAALGSPALLPPL → VRFQAGHKDPDSSRELQLSTLPILPALVSPSLKGKLAWYFSGSLLRAVHLPPH
Q9BRQ3-3
- Name3
- Differences from canonical
- 160-193: QALCPGGSPQHQDLAGQLVVHELFSSVLQEICDE → Q
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Features
Showing features for sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 9 | in Ref. 1; AAQ15238 | ||||
Sequence: L → V | ||||||
Alternative sequence | VSP_053790 | 160-193 | in isoform 3 | |||
Sequence: QALCPGGSPQHQDLAGQLVVHELFSSVLQEICDE → Q | ||||||
Alternative sequence | VSP_021884 | 161-303 | in isoform 2 | |||
Sequence: ALCPGGSPQHQDLAGQLVVHELFSSVLQEICDEVNLPLLTLSQPLLLGIARNETSAGRASAEFYVQCSLTSEQVRKHYLSGGPEAHESTGIFFVETQNVQRLLETEMWAELCPSAKGAIILYNRVQGSPTGAALGSPALLPPL → VRFQAGHKDPDSSRELQLSTLPILPALVSPSLKGKLAWYFSGSLLRAVHLPPH |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF370402 EMBL· GenBank· DDBJ | AAQ15238.1 EMBL· GenBank· DDBJ | mRNA | ||
AP001453 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AP006334 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC006129 EMBL· GenBank· DDBJ | AAH06129.1 EMBL· GenBank· DDBJ | mRNA |