Q9AFC7 · GTFD_AMYOR

Function

function

Catalyzes the attachment of L-vancosamine to a monoglucosylated heptapeptide intermediate during the final stage of glycopeptide antibiotic vancomycin biosynthesis.

Miscellaneous

In A.orientalis different glycosyltransferases are involved in biosynthesis of the vancomycin group of antibiotics. GtfA (AC P96558), GtfB (AC P96559) and GtfC (AC P96560) are involved in biosynthesis of antibiotic chloroeremomycin, while GtfD and GtfE (AC G4V4R9) are involved in biosynthesis of vancomycin.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
16.3 μMdevancoaminyl-vancomycin
2 mMUDP-L-epivancosamine
kcat is 67.6 min-1.

Pathway

Antibiotic biosynthesis; vancomycin biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site10-12dTDP (UniProtKB | ChEBI)
Binding site13beta-D-glucose (UniProtKB | ChEBI)
Binding site76devancoaminyl-vancomycin (UniProtKB | ChEBI)
Binding site80devancoaminyl-vancomycin (UniProtKB | ChEBI)
Binding site103beta-D-glucose (UniProtKB | ChEBI)
Binding site246dTDP (UniProtKB | ChEBI)
Binding site293-296dTDP (UniProtKB | ChEBI)
Binding site309-314dTDP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionhexosyltransferase activity
Molecular FunctionUDP-glycosyltransferase activity
Biological Processcarbohydrate metabolic process
Biological Processlipid glycosylation
Biological Processvancomycin biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

    • GT1Glycosyltransferase Family 1

Names & Taxonomy

Protein names

  • Recommended name
    Devancosaminyl-vancomycin vancosaminetransferase
  • EC number
  • Alternative names
    • Devancosamine-vancomycin TDP-vancosaminyltransferase
    • Glycosyltransferase GtfD

Gene names

    • Name
      gtfD

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 19795 / DSM 40040 / CBS 547.68 / JCM 4235 / KCTC 9412 / NBRC 12806 / NCIMB 12945 / M43-05865
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Pseudonocardiales > Pseudonocardiaceae > Amycolatopsis

Accessions

  • Primary accession
    Q9AFC7

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis10Strongly decreased activity.
Mutagenesis13Abolishes catalytic activity.

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004304391-408Devancosaminyl-vancomycin vancosaminetransferase

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the glycosyltransferase 28 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    408
  • Mass (Da)
    42,881
  • Last updated
    2001-06-01 v1
  • Checksum
    1233D71CD99D1FBD
MRVLLSVCGTRGDVEIGVALADRLKALGVQTRMCAPPAAEERLAEVGVPHVPVGLPQHMMLQEGMPPPPPEEEQRLAAMTVEMQFDAVPGAAEGCAAVVAVGDLAAATGVRSVAEKLGLPFFYSVPSPVYLASPHLPPAYDEPTTPGVTDIRVLWEERAARFADRYGPTLNRRRAEIGLPPVEDVFGYGHGERPCWPADPVLAPLQPDVDAVQTGAWLLSDERPLPPELEAFLAAGSPPVHIGFGSSSGRGIADAAKVAVEAIRAQGRRVILSRGWTELVLPDDRDDCFAIDEVNFQALFRRVAAVIHHGSAGTEHVATRAGVPQLVIPRNTDQPYFAGRVAALGIGVAHDGPTPTFESLSAALTTVLAPETRARAEAVAGMVLTDGAAAAADLVLAAVGREKPAVPA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF351621
EMBL· GenBank· DDBJ
AAK31352.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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