Q9AFC7 · GTFD_AMYOR
- ProteinDevancosaminyl-vancomycin vancosaminetransferase
- GenegtfD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids408 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the attachment of L-vancosamine to a monoglucosylated heptapeptide intermediate during the final stage of glycopeptide antibiotic vancomycin biosynthesis.
Miscellaneous
In A.orientalis different glycosyltransferases are involved in biosynthesis of the vancomycin group of antibiotics. GtfA (AC P96558), GtfB (AC P96559) and GtfC (AC P96560) are involved in biosynthesis of antibiotic chloroeremomycin, while GtfD and GtfE (AC G4V4R9) are involved in biosynthesis of vancomycin.
Catalytic activity
- dTDP-beta-L-vancosamine + devancoaminyl-vancomycin = vancomycin + dTDP + H+
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
16.3 μM | devancoaminyl-vancomycin | |||||
2 mM | UDP-L-epivancosamine |
kcat is 67.6 min-1.
Pathway
Antibiotic biosynthesis; vancomycin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 10-12 | dTDP (UniProtKB | ChEBI) | ||||
Sequence: TRG | ||||||
Binding site | 13 | beta-D-glucose (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 76 | devancoaminyl-vancomycin (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 80 | devancoaminyl-vancomycin (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 103 | beta-D-glucose (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 246 | dTDP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 293-296 | dTDP (UniProtKB | ChEBI) | ||||
Sequence: EVNF | ||||||
Binding site | 309-314 | dTDP (UniProtKB | ChEBI) | ||||
Sequence: HGSAGT |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | hexosyltransferase activity | |
Molecular Function | UDP-glycosyltransferase activity | |
Biological Process | carbohydrate metabolic process | |
Biological Process | lipid glycosylation | |
Biological Process | vancomycin biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameDevancosaminyl-vancomycin vancosaminetransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Pseudonocardiales > Pseudonocardiaceae > Amycolatopsis
Accessions
- Primary accessionQ9AFC7
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 10 | Strongly decreased activity. | ||||
Sequence: T → A | ||||||
Mutagenesis | 13 | Abolishes catalytic activity. | ||||
Sequence: D → A |
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000430439 | 1-408 | Devancosaminyl-vancomycin vancosaminetransferase | |||
Sequence: MRVLLSVCGTRGDVEIGVALADRLKALGVQTRMCAPPAAEERLAEVGVPHVPVGLPQHMMLQEGMPPPPPEEEQRLAAMTVEMQFDAVPGAAEGCAAVVAVGDLAAATGVRSVAEKLGLPFFYSVPSPVYLASPHLPPAYDEPTTPGVTDIRVLWEERAARFADRYGPTLNRRRAEIGLPPVEDVFGYGHGERPCWPADPVLAPLQPDVDAVQTGAWLLSDERPLPPELEAFLAAGSPPVHIGFGSSSGRGIADAAKVAVEAIRAQGRRVILSRGWTELVLPDDRDDCFAIDEVNFQALFRRVAAVIHHGSAGTEHVATRAGVPQLVIPRNTDQPYFAGRVAALGIGVAHDGPTPTFESLSAALTTVLAPETRARAEAVAGMVLTDGAAAAADLVLAAVGREKPAVPA |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length408
- Mass (Da)42,881
- Last updated2001-06-01 v1
- Checksum1233D71CD99D1FBD
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF351621 EMBL· GenBank· DDBJ | AAK31352.1 EMBL· GenBank· DDBJ | Genomic DNA |