Q99PP6 · TR34A_MOUSE
- ProteinE3 ubiquitin-protein ligase TRIM34A
- GeneTrim34a
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids485 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Functions as antiviral protein and contributes to the defense against retroviral infections (By similarity).
Acts as a capsid-specific restriction factor with the help of TRIM5 and prevents infection from non-host-adapted retroviruses. During influenza A virus infection, promotes programmed cell death by targeting ZBP1 for 'Lys-63'-linked polyubiquitination. In turn, promotes ZBP1 recruitment of RIPK3 to mediate virus-induced programmed necrosis (By similarity).
Negatively regulates the function of mitochondria by enhancing mitochondrial depolarization leading to cytochrome c release and mitochondria-dependent apoptosis. Promotes also the formation of multinucleated giant cells by means of cell fusion and phagocytosis in epithelial cells (By similarity).
Plays an essential role in sustaining the integrity of the inner mucus layer in the colon by controlling the exocytosis of the major component of colonic mucus MUC2 from colonic goblet cells (PubMed:32094504).
Acts as a capsid-specific restriction factor with the help of TRIM5 and prevents infection from non-host-adapted retroviruses. During influenza A virus infection, promotes programmed cell death by targeting ZBP1 for 'Lys-63'-linked polyubiquitination. In turn, promotes ZBP1 recruitment of RIPK3 to mediate virus-induced programmed necrosis (By similarity).
Negatively regulates the function of mitochondria by enhancing mitochondrial depolarization leading to cytochrome c release and mitochondria-dependent apoptosis. Promotes also the formation of multinucleated giant cells by means of cell fusion and phagocytosis in epithelial cells (By similarity).
Plays an essential role in sustaining the integrity of the inner mucus layer in the colon by controlling the exocytosis of the major component of colonic mucus MUC2 from colonic goblet cells (PubMed:32094504).
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Cellular Component | nucleoplasm | |
Molecular Function | protein homodimerization activity | |
Molecular Function | protein kinase binding | |
Molecular Function | ubiquitin protein ligase activity | |
Molecular Function | zinc ion binding | |
Biological Process | defense response to virus | |
Biological Process | innate immune response | |
Biological Process | positive regulation of autophagy | |
Biological Process | positive regulation of canonical NF-kappaB signal transduction | |
Biological Process | protein polyubiquitination | |
Biological Process | regulation of protein localization | |
Biological Process | regulation of viral entry into host cell |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameE3 ubiquitin-protein ligase TRIM34A
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ99PP6
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Trim34a- and TRIM34b-deficient mice show an impaired integrity of the inner mucus layer.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 51 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000056249 | 1-485 | E3 ubiquitin-protein ligase TRIM34A | |||
Sequence: MASTGLTNIQEKTTCPVCQELLTKALSLGCGHRVCQACLITKKNAVINPREKSSCPVCGTRFSLENLQANKHLANVVERLGEVKLKPDIGTKRDLCVHHGEKLLLFCKEDKKAICWVCERSQEHRGHHTFLWEEAVRECQENLQKALTRLRKEQEKVETLEADIKEDRLSWKCQVQTERQRIQTGFNQLRRILDKEEQRELKRLREEEQMILDSLAGAEAELAQQSQLVEELISDLELRREWSDTELLQDMSGILKWSQIWTLKKPKAVSKKLSMVFQAPDLSGMLQKFRELTAVRAYWDNFTFNPENLNLNLILSEDHRQVTSVSIWPFKCCNNGILGSKCFSSGKHYWEVDVSEKKAWTLGVYTRKRTLRFDVRQRKGQPNGYHRYKPQNGYWVIGLQHGSKYSIFEDSSNCDPTVLNPFVATPLHRVGVFLDCEEGTVSFLNVTNHGSLIYKFSQCCFSQPAYPYFNPWDCPAPMTLCPLNS |
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for zinc finger, coiled coil, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 15-59 | RING-type | ||||
Sequence: CPVCQELLTKALSLGCGHRVCQACLITKKNAVINPREKSSCPVCG | ||||||
Zinc finger | 91-132 | B box-type | ||||
Sequence: TKRDLCVHHGEKLLLFCKEDKKAICWVCERSQEHRGHHTFLW | ||||||
Coiled coil | 136-170 | |||||
Sequence: VRECQENLQKALTRLRKEQEKVETLEADIKEDRLS | ||||||
Domain | 282-485 | B30.2/SPRY | ||||
Sequence: LSGMLQKFRELTAVRAYWDNFTFNPENLNLNLILSEDHRQVTSVSIWPFKCCNNGILGSKCFSSGKHYWEVDVSEKKAWTLGVYTRKRTLRFDVRQRKGQPNGYHRYKPQNGYWVIGLQHGSKYSIFEDSSNCDPTVLNPFVATPLHRVGVFLDCEEGTVSFLNVTNHGSLIYKFSQCCFSQPAYPYFNPWDCPAPMTLCPLNS |
Sequence similarities
Belongs to the TRIM/RBCC family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q99PP6-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameAlpha
- Length485
- Mass (Da)55,950
- Last updated2022-12-14 v3
- ChecksumA9B5F3590C9A2614
Q99PP6-2
- NameBeta
Q99PP6-3
- NameGamma
Features
Showing features for sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 293 | in Ref. 1; AAG53514/AAG53513 | ||||
Sequence: T → S | ||||||
Alternative sequence | VSP_011925 | 300-326 | in isoform Gamma | |||
Sequence: DNFTFNPENLNLNLILSEDHRQVTSVS → GSYSVYKVGFKYRAIFLPQLPRCSTAG | ||||||
Alternative sequence | VSP_011923 | 300-339 | in isoform Beta | |||
Sequence: DNFTFNPENLNLNLILSEDHRQVTSVSIWPFKCCNNGILG → GKKLQMLKSLCSDHLSQVCGSMWPYHLCLLCYLIHIMNSS | ||||||
Alternative sequence | VSP_011926 | 327-485 | in isoform Gamma | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_011924 | 340-485 | in isoform Beta | |||
Sequence: Missing | ||||||
Sequence conflict | 430 | in Ref. 1; AAG53512 | ||||
Sequence: V → A |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF220139 EMBL· GenBank· DDBJ | AAG53512.1 EMBL· GenBank· DDBJ | mRNA | ||
AF220140 EMBL· GenBank· DDBJ | AAG53513.1 EMBL· GenBank· DDBJ | mRNA | ||
AF220141 EMBL· GenBank· DDBJ | AAG53514.1 EMBL· GenBank· DDBJ | mRNA | ||
AC123830 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |