Q99PN0 · SC5A5_MOUSE
- ProteinSodium/iodide cotransporter
- GeneSlc5a5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids618 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Sodium:iodide symporter that mediates the transport of iodide into the thyroid gland (PubMed:11716040, PubMed:18372236, PubMed:32084174).
Can also mediate the transport of chlorate, thiocynate, nitrate and selenocynate (By similarity).
Can also mediate the transport of chlorate, thiocynate, nitrate and selenocynate (By similarity).
Catalytic activity
- iodide(out) + 2 Na+(out) = iodide(in) + 2 Na+(in)
- chlorate(out) + 2 Na+(out) = chlorate(in) + 2 Na+(in)
- 2 Na+(out) + thiocyanate(out) = 2 Na+(in) + thiocyanate(in)
- 2 Na+(out) + nitrate(out) = 2 Na+(in) + nitrate(in)
- 2 Na+(out) + selenocyanate(out) = 2 Na+(in) + selenocyanate(in)
Activity regulation
Perchlorate inhibits iodide transport activity.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
26 μM | iodide |
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 242 | Required for homodimerization | ||||
Sequence: Y | ||||||
Site | 243 | Required for homodimerization | ||||
Sequence: T | ||||||
Site | 471 | Required for homodimerization | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | plasma membrane | |
Molecular Function | iodide transmembrane transporter activity | |
Molecular Function | sodium:iodide symporter activity | |
Molecular Function | symporter activity | |
Biological Process | iodide transmembrane transport | |
Biological Process | iodide transport | |
Biological Process | sodium ion transport | |
Biological Process | thyroid hormone generation |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSodium/iodide cotransporter
- Short namesNa(+)/I(-) cotransporter
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ99PN0
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-16 | Extracellular | ||||
Sequence: MEGAEAGARATFGPWD | ||||||
Transmembrane | 17-37 | Helical | ||||
Sequence: YGVFATMLLVSTGIGLWVGLA | ||||||
Topological domain | 38-53 | Cytoplasmic | ||||
Sequence: RGGQRSADDFFTGGRQ | ||||||
Transmembrane | 54-74 | Helical | ||||
Sequence: LAAVPVGLSLAASFMSAVQVL | ||||||
Topological domain | 75-88 | Extracellular | ||||
Sequence: GVPAEAARYGLKFL | ||||||
Transmembrane | 89-109 | Helical | ||||
Sequence: WMCVGQLLNSLLTALLFLPIF | ||||||
Topological domain | 110-136 | Cytoplasmic | ||||
Sequence: YRLGLTSTYQYLELRFSRAVRLCGTLQ | ||||||
Transmembrane | 137-157 | Helical | ||||
Sequence: YLVATMLYTGIVIYAPALILN | ||||||
Topological domain | 158-163 | Extracellular | ||||
Sequence: QVTGLD | ||||||
Transmembrane | 164-184 | Helical | ||||
Sequence: IWASLLSTGIICTLYTTVGGM | ||||||
Topological domain | 185-186 | Cytoplasmic | ||||
Sequence: KA | ||||||
Transmembrane | 187-207 | Helical | ||||
Sequence: VVWTDVFQVVVMLVGFWVILA | ||||||
Topological domain | 208-241 | Extracellular | ||||
Sequence: RGVMLMGGPWNVLSLAQNHSRINLMDFDPDPRSR | ||||||
Transmembrane | 242-262 | Helical | ||||
Sequence: YTFWTFVVGGSLVWLSMYGVN | ||||||
Topological domain | 263-286 | Cytoplasmic | ||||
Sequence: QAQVQRYVACHTERKAKLALLVNQ | ||||||
Transmembrane | 287-307 | Helical | ||||
Sequence: LGLFLIVASAACCGIVMFVYY | ||||||
Topological domain | 308-326 | Extracellular | ||||
Sequence: KDCDPLLTGRIAAPDQYMP | ||||||
Transmembrane | 327-347 | Helical | ||||
Sequence: LLVLDIFEDLPGVPGLFLACA | ||||||
Topological domain | 348-391 | Cytoplasmic | ||||
Sequence: YSGTLSTASTSINAMAAVTVEDLIKPRMPSLAPRKLVFISKGLS | ||||||
Transmembrane | 392-412 | Helical | ||||
Sequence: FIYGSTCLTVAALSSLLGGGV | ||||||
Topological domain | 413-416 | Extracellular | ||||
Sequence: LQGS | ||||||
Transmembrane | 417-437 | Helical | ||||
Sequence: FTVMGVISGPLLGAFTLGMLL | ||||||
Topological domain | 438-444 | Cytoplasmic | ||||
Sequence: PACNTPG | ||||||
Transmembrane | 445-465 | Helical | ||||
Sequence: VLSGLTAGLAVSLWVAVGATL | ||||||
Topological domain | 466-520 | Extracellular | ||||
Sequence: YPPGEQTMGVLPTSAAGCTNASVLPSPPGAANTSRGIPSSGMDSGRPAFADTFYA | ||||||
Transmembrane | 521-541 | Helical | ||||
Sequence: VSYLYYGALGTLTTMLCGALI | ||||||
Topological domain | 542-618 | Cytoplasmic | ||||
Sequence: SYLTGPTKRSSLGPGLLWWDLARQTASVAPKEDTTTLEDSLVKGPEDIPAATKKPPGFRPEAETHPLYLGHDVETNL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000105384 | 1-618 | Sodium/iodide cotransporter | |||
Sequence: MEGAEAGARATFGPWDYGVFATMLLVSTGIGLWVGLARGGQRSADDFFTGGRQLAAVPVGLSLAASFMSAVQVLGVPAEAARYGLKFLWMCVGQLLNSLLTALLFLPIFYRLGLTSTYQYLELRFSRAVRLCGTLQYLVATMLYTGIVIYAPALILNQVTGLDIWASLLSTGIICTLYTTVGGMKAVVWTDVFQVVVMLVGFWVILARGVMLMGGPWNVLSLAQNHSRINLMDFDPDPRSRYTFWTFVVGGSLVWLSMYGVNQAQVQRYVACHTERKAKLALLVNQLGLFLIVASAACCGIVMFVYYKDCDPLLTGRIAAPDQYMPLLVLDIFEDLPGVPGLFLACAYSGTLSTASTSINAMAAVTVEDLIKPRMPSLAPRKLVFISKGLSFIYGSTCLTVAALSSLLGGGVLQGSFTVMGVISGPLLGAFTLGMLLPACNTPGVLSGLTAGLAVSLWVAVGATLYPPGEQTMGVLPTSAAGCTNASVLPSPPGAANTSRGIPSSGMDSGRPAFADTFYAVSYLYYGALGTLTTMLCGALISYLTGPTKRSSLGPGLLWWDLARQTASVAPKEDTTTLEDSLVKGPEDIPAATKKPPGFRPEAETHPLYLGHDVETNL | ||||||
Glycosylation | 485 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 497 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 551 | Phosphoserine; by PKA | ||||
Sequence: S |
Post-translational modification
Glycosylated.
Keywords
- PTM
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 571-618 | Disordered | ||||
Sequence: PKEDTTTLEDSLVKGPEDIPAATKKPPGFRPEAETHPLYLGHDVETNL |
Sequence similarities
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length618
- Mass (Da)65,568
- Last updated2001-06-01 v1
- Checksum448AC5FE9AB5118E
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
G3X8P5 | G3X8P5_MOUSE | Slc5a5 | 618 |
Keywords
- Technical term