Q99MU0 · DBF4A_CRIGR

Function

function

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle (By similarity).

Features

Showing features for binding site.

167650100150200250300350400450500550600650
TypeIDPosition(s)Description
Binding site296Zn2+ (UniProtKB | ChEBI)
Binding site299Zn2+ (UniProtKB | ChEBI)
Binding site309Zn2+ (UniProtKB | ChEBI)
Binding site315Zn2+ (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular ComponentDbf4-dependent protein kinase complex
Molecular Functionnucleic acid binding
Molecular Functionprotein serine/threonine kinase activator activity
Molecular Functionzinc ion binding
Biological ProcessDNA replication
Biological Processpositive regulation of nuclear cell cycle DNA replication
Biological Processregulation of cell cycle phase transition

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Protein DBF4 homolog A
  • Alternative names
    • ChDBF4

Gene names

    • Name
      DBF4
    • Synonyms
      DBF4A

Organism names

Accessions

  • Primary accession
    Q99MU0

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002340601-676Protein DBF4 homolog A
Modified residue273Phosphothreonine
Modified residue312Phosphoserine
Modified residue345Phosphothreonine
Modified residue354Phosphoserine
Modified residue359Phosphoserine
Modified residue418Phosphoserine
Modified residue627Phosphoserine
Modified residue669Phosphoserine
Modified residue671Phosphoserine

Post-translational modification

Phosphorylation increases its interaction with PSIP1.

Keywords

Proteomic databases

Expression

Induction

Up-regulated when cells reaches the G1/S boundary. Down-regulated by gamma-irradiation.

Interaction

Subunit

Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6 (By similarity).
Interacts (via IBM motifs) with PSIP1 (via IBD domain); phosphorylation increases its affinity for PSIP1 (By similarity).

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain, zinc finger, motif.

TypeIDPosition(s)Description
Region1-40Disordered
Compositional bias26-40Basic and acidic residues
Domain40-128BRCT 1
Compositional bias98-125Polar residues
Region98-132Disordered
Domain154-179BRCT 2
Region253-281Disordered
Zinc finger289-337DBF4-type
Motif616-640Integrase domain-binding motif 1 (IBM1)
Motif657-676Integrase domain-binding motif 2 (IBM2)

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    676
  • Mass (Da)
    75,850
  • Last updated
    2001-06-01 v1
  • Checksum
    EC7EEBDD80D40B8C
MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWEKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLVSNKKEAKYAQTLGQVSPVPSPESAYTAETTSPHPSHDGSSFKSPDRVCLSRGKLLAEKAVKDHDFIPANSILSNALTWGVKILHIDDIRYYIEQKKKELCSLKKSSTSVRDSGKKAGTTIQKARTGRLKKPFLKVEDVNRSYRPFYLQLTSVPSINYATHKPCSPFDIEKPSSVQKQAQPKPRPNTDGDKCGGTPVQLQLKEKRKKGYCECCLQKYEDLETHLLSEKHKNFAQSNQYQVVDDIVSKLVFDFVEYERDTPKKKRIKYSVGSFSSVSANVLKNTEPKEKLQLEPIFQKDMVESNGQLPEEIFQCEDIQCEDIQKPEQRLVLASEPMSYSSTGLKGRDEKAASMLNASEPDIKQKFTQLPPCKNEQEGILDVSEHKLIINRNDLEQRVGDSVGVPRSCVQVSHLSPENSLPQPKLTADTTHFSAKDLQEKDLHFVFGHDSDLVTLNTSKEQLTVKAGTPSCGPQQPNECDTENTDNLPCGKIQRKVRLLLGQKKKNVDPSAELDKKRTEFLPMCEDRTCGSPVQSLLDLFQTSGEKSDFLGFTSYTENSGLCDVLDVWEDENSSSLLSTFFSSPSASTFIGF

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias26-40Basic and acidic residues
Compositional bias98-125Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF292400
EMBL· GenBank· DDBJ
AAK21856.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp