Q99KD6 · CO72R_MOUSE
- ProteinCytochrome c oxidase subunit 7A2-like, mitochondrial
- GeneCox7a2l
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids113 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Assembly factor that mediates the formation of some mitochondrial respiratory supercomplexes (respirasomes), thereby promoting oxidative phosphorylation and energy metabolism (PubMed:23812712, PubMed:23857330, PubMed:25470551, PubMed:27545886, PubMed:27775717, PubMed:27997587, PubMed:32637615, PubMed:34616041, PubMed:38575788).
Acts as a molecular adapter that associates with both mitochondrial respiratory complexes III (CIII) and IV (CIV), promoting their association (PubMed:27545886, PubMed:27775717, PubMed:32637615, PubMed:34616041, PubMed:38575788).
Mediates the formation of various mitochondrial respiratory supercomplexes, such as MCIII2IV2, composed of two CIII and two CIV, and the CS-respirasome (MCI1III2IV2), composed of one CI, two CIII and two CIV (PubMed:27775717, PubMed:32637615, PubMed:34616041, PubMed:38575788).
Not involved in the formation of the canonical respirasome (MCI1III2IV1), composed of one CI, two CIII and one CIV (PubMed:38575788).
The formation of different respirasomes is important for cell adaptation to oxygen conditions and prevent metabolic exhaustion: supercomplexes mediated by COX7A2L/SCAF1 are required to maintain oxidative phosphorylation upon low oxygen conditions and promote metabolic rewiring toward glycolysis (PubMed:38575788).
Acts as a molecular adapter that associates with both mitochondrial respiratory complexes III (CIII) and IV (CIV), promoting their association (PubMed:27545886, PubMed:27775717, PubMed:32637615, PubMed:34616041, PubMed:38575788).
Mediates the formation of various mitochondrial respiratory supercomplexes, such as MCIII2IV2, composed of two CIII and two CIV, and the CS-respirasome (MCI1III2IV2), composed of one CI, two CIII and two CIV (PubMed:27775717, PubMed:32637615, PubMed:34616041, PubMed:38575788).
Not involved in the formation of the canonical respirasome (MCI1III2IV1), composed of one CI, two CIII and one CIV (PubMed:38575788).
The formation of different respirasomes is important for cell adaptation to oxygen conditions and prevent metabolic exhaustion: supercomplexes mediated by COX7A2L/SCAF1 are required to maintain oxidative phosphorylation upon low oxygen conditions and promote metabolic rewiring toward glycolysis (PubMed:38575788).
Pathway
Energy metabolism; oxidative phosphorylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial inner membrane | |
Cellular Component | respiratory chain complex IV | |
Biological Process | mitochondrial electron transport, cytochrome c to oxygen |
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome c oxidase subunit 7A2-like, mitochondrial
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ99KD6
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion inner membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 81-106 | Helical | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Knockout homozygous mice manifest decreased mitochondrial respiratory activities and impaired formation of mitochondrial supercomplexes in muscles (PubMed:23857330).
Mice display muscle weakness and exhibit heat production failure in the cold (PubMed:23857330).
Mice display muscle weakness and exhibit heat production failure in the cold (PubMed:23857330).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 75 | Abolished ability to promote association between both mitochondrial respiratory complexes III (CIII) and IV (CIV). | |||
PTM/Processing
Features
Showing features for transit peptide, chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transit peptide | 1-54 | Mitochondrion | |||
Chain | PRO_0000461461 | 55-113 | Cytochrome c oxidase subunit 7A2-like, mitochondrial | ||
Modified residue | 68 | N6-acetyllysine | |||
Keywords
- PTM
Interaction
Subunit
Interacts with the mitochondrial respiratory complexes III (CIII) and IV (CIV), promoting their association.
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length113
- Mass (Da)12,562
- Last updated2001-06-01 v1
- MD5 ChecksumBE37C3A8914C7EA9F0A92160B327D9C4
Polymorphism
There are two alleles for Cox7a2l/Scaf1 depending on mouse strains; a 113 amino-acid long protein, which corresponds to a functional protein (this protein), and a 111 amino-acid long protein caused by a two-amino acid deletion (AC Q61387) (PubMed:23812712, PubMed:36253618).
Only the 113 amino-acid form (this proteins) can induce the mitochondrial respiratory supercomplex assembly by promoting interaction between complex III (CIII) and complex IV (CIV) (PubMed:23812712, PubMed:36253618).
Only the 113 amino-acid form (this proteins) can induce the mitochondrial respiratory supercomplex assembly by promoting interaction between complex III (CIII) and complex IV (CIV) (PubMed:23812712, PubMed:36253618).
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC004715 EMBL· GenBank· DDBJ | AAH04715.1 EMBL· GenBank· DDBJ | mRNA | ||
BC085296 EMBL· GenBank· DDBJ | AAH85296.1 EMBL· GenBank· DDBJ | mRNA |